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{sup 99m}Tc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques

Abstract

Several histopathological studies have demonstrated that vulnerable plaques are enriched in inflammatory cells. The aims of this study were: (1a) to test the ability of {sup 99m}Tc-labelled interleukin-2 ({sup 99m}Tc-IL2) to bind to IL2R-positive (IL2R+) cells in carotid plaques and (1b) to correlate the plaque uptake of {sup 99m}Tc-IL2, measured in vivo, with the number of IL2R+ cells within the plaque, measured ex vivo by histology (transversal study, TS), and (2) to evaluate changes in {sup 99m}Tc-IL2 uptake in plaques, before and after treatment with a statin or a hypocholesterolaemic diet (longitudinal study, LS). Ultrasound scan was performed for plaque characterisation and localisation. Fourteen patients (16 plaques) eligible for endoarterectomy were recruited for the TS and underwent {sup 99m}Tc-IL2 scintigraphy before surgery. Nine patients (13 plaques) were recruited for the LS; these patients received atorvastatin or a standard hypocholesterolaemic diet and {sup 99m}Tc-IL2 scintigraphy was performed before and after 3 months of treatment. The degree of {sup 99m}Tc-IL2 uptake was expressed as the plaque/background (T/B) ratio. In patients from TS, T/B ratios correlated with the percentage of IL2R+ cells at histology (r=0.707; p=0.002) and the number of IL2R+ cells at flow cytometry (r=0.711; p=0.006). No correlations were observed between ultrasound  More>>
Authors:
Annovazzi, Alessio; D'Alessandria, Calogero; Scopinaro, Francesco; [1]  Bonanno, Elena; Spagnoli, Luigi G; [2]  Arca, Marcello; [3]  Marcoccia, Antonella; Violi, Francesco; [4]  Toma, Giorgio De; [5]  Signore, Alberto; [1]  University of Groningen, Department of Nuclear Medicine, Groningen (Netherlands); Ospedale S. Andrea, Nuclear Medicine, Roma (Italy)]
  1. University La Sapienza, Nuclear Medicine, 2nd Faculty of Medicine, Rome (Italy)
  2. University Tor Vergata, Department of Biopathology and Diagnostic Imaging, Rome (Italy)
  3. University La Sapienza, Department of Clinical and Applied Medical Therapy, 1st Faculty of Medicine, Rome (Italy)
  4. University La Sapienza, Medical Clinical Institute 1, 1st Faculty of Medicine, Rome (Italy)
  5. University La Sapienza, Department of Surgery Pietro Valdoni, 1st Faculty of Medicine, Rome (Italy)
Publication Date:
Feb 01, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: European Journal of Nuclear Medicine and Molecular Imaging; Journal Volume: 33; Journal Issue: 2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ARTERIOSCLEROSIS; CAROTID ARTERIES; HISTOLOGY; IMAGES; IN VIVO; ISOMERIC NUCLEI; NMR IMAGING; PATHOLOGICAL CHANGES; PATHOLOGY; TECHNETIUM 99
OSTI ID:
20707828
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1619-7070; TRN: DE06F3486
Availability:
Available from: http://dx.doi.org/10.1007/s00259-005-1899-4
Submitting Site:
DEN
Size:
page(s) 117-126
Announcement Date:
Apr 12, 2006

Citation Formats

Annovazzi, Alessio, D'Alessandria, Calogero, Scopinaro, Francesco, Bonanno, Elena, Spagnoli, Luigi G, Arca, Marcello, Marcoccia, Antonella, Violi, Francesco, Toma, Giorgio De, Signore, Alberto, University of Groningen, Department of Nuclear Medicine, Groningen (Netherlands), and Ospedale S. Andrea, Nuclear Medicine, Roma (Italy)]. {sup 99m}Tc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques. Germany: N. p., 2006. Web. doi:10.1007/s00259-005-1899-4.
Annovazzi, Alessio, D'Alessandria, Calogero, Scopinaro, Francesco, Bonanno, Elena, Spagnoli, Luigi G, Arca, Marcello, Marcoccia, Antonella, Violi, Francesco, Toma, Giorgio De, Signore, Alberto, University of Groningen, Department of Nuclear Medicine, Groningen (Netherlands), & Ospedale S. Andrea, Nuclear Medicine, Roma (Italy)]. {sup 99m}Tc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques. Germany. doi:10.1007/s00259-005-1899-4.
Annovazzi, Alessio, D'Alessandria, Calogero, Scopinaro, Francesco, Bonanno, Elena, Spagnoli, Luigi G, Arca, Marcello, Marcoccia, Antonella, Violi, Francesco, Toma, Giorgio De, Signore, Alberto, University of Groningen, Department of Nuclear Medicine, Groningen (Netherlands), and Ospedale S. Andrea, Nuclear Medicine, Roma (Italy)]. 2006. "{sup 99m}Tc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques." Germany. doi:10.1007/s00259-005-1899-4. https://www.osti.gov/servlets/purl/10.1007/s00259-005-1899-4.
@misc{etde_20707828,
title = {{sup 99m}Tc-interleukin-2 scintigraphy for the in vivo imaging of vulnerable atherosclerotic plaques}
author = {Annovazzi, Alessio, D'Alessandria, Calogero, Scopinaro, Francesco, Bonanno, Elena, Spagnoli, Luigi G, Arca, Marcello, Marcoccia, Antonella, Violi, Francesco, Toma, Giorgio De, Signore, Alberto, University of Groningen, Department of Nuclear Medicine, Groningen (Netherlands), and Ospedale S. Andrea, Nuclear Medicine, Roma (Italy)]}
abstractNote = {Several histopathological studies have demonstrated that vulnerable plaques are enriched in inflammatory cells. The aims of this study were: (1a) to test the ability of {sup 99m}Tc-labelled interleukin-2 ({sup 99m}Tc-IL2) to bind to IL2R-positive (IL2R+) cells in carotid plaques and (1b) to correlate the plaque uptake of {sup 99m}Tc-IL2, measured in vivo, with the number of IL2R+ cells within the plaque, measured ex vivo by histology (transversal study, TS), and (2) to evaluate changes in {sup 99m}Tc-IL2 uptake in plaques, before and after treatment with a statin or a hypocholesterolaemic diet (longitudinal study, LS). Ultrasound scan was performed for plaque characterisation and localisation. Fourteen patients (16 plaques) eligible for endoarterectomy were recruited for the TS and underwent {sup 99m}Tc-IL2 scintigraphy before surgery. Nine patients (13 plaques) were recruited for the LS; these patients received atorvastatin or a standard hypocholesterolaemic diet and {sup 99m}Tc-IL2 scintigraphy was performed before and after 3 months of treatment. The degree of {sup 99m}Tc-IL2 uptake was expressed as the plaque/background (T/B) ratio. In patients from TS, T/B ratios correlated with the percentage of IL2R+ cells at histology (r=0.707; p=0.002) and the number of IL2R+ cells at flow cytometry (r=0.711; p=0.006). No correlations were observed between ultrasound scores and either scintigraphic or histological findings. In patients from the LS, the mean {sup 99m}Tc-IL2 uptake decreased in statin-treated patients (1.75{+-}0.50 vs 2.16{+-}0.44; p=0.012), while it was unchanged in the patients on the hypocholesterolaemic diet (2.33{+-}0.45 vs 2.34{+-}0.5). {sup 99m}Tc-IL2 accumulates in vulnerable carotid plaques; this accumulation is correlated with the amount of IL2R+ cells and is influenced by lipid-lowering treatment with a statin. (orig.)}
doi = {10.1007/s00259-005-1899-4}
journal = {European Journal of Nuclear Medicine and Molecular Imaging}
issue = {2}
volume = {33}
place = {Germany}
year = {2006}
month = {Feb}
}