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Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial

Abstract

A historical review describes the reticulo-endothelial system (R.E.S.) and aims at defining it and at explaining its operation. The methods used for its examination and the colloids utilized are considered. The author has been led to prepare a special type of colloid: an albuminous complex containing radio-iodine, 'C.A. {sup 131}I', whose method of preparation and physical and biological examination are described. A human albumin, having a known optical density in solution, is heated until a change in the optical density indicates that an aggregation of the proteinic molecules has occurred. The denatured protein is iodated with {sup 131}I. Electrophoretic, ultracentrifuge and autoradiographic controls are then carried out. This atoxic and metabolisable preparation of biological origin is compared with the better defined colloidal gold which serves as reference. C.A.{sup 131}I is injected into mice. It is shown by radioactivity measurements, auto-radiographies on sections of the whole animal, and anthropo-gamma-metric detections that a high concentration occurs in the S.R.E. of the liver. These static results are only of limited importance however compared to those obtained from an in vivo study of the phenomenon. The author records the changes in the radioactivity of the blood derived from the carotid artery using a well-scintillator.  More>>
Authors:
Chivot, J J [1] 
  1. Commissariat a l'Energie Atomique, Saclay (France). Centre d'Etudes Nucleaires
Publication Date:
Mar 01, 1967
Product Type:
Thesis/Dissertation
Report Number:
CEA-R-3240
Resource Relation:
Other Information: 161 refs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ALBUMINS; AUTORADIOGRAPHY; CHEMICAL PREPARATION; COLLOIDS; ELECTROPHORESIS; IODINE 131; MICE; PHAGOCYTOSIS; RETICULOENDOTHELIAL SYSTEM; ULTRACENTRIFUGATION
OSTI ID:
20653430
Research Organizations:
CEA Saclay, 91 - Gif-sur-Yvette (France); Faculte de Pharmacie de Paris, 75 (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
TRN: FR05R3240093015
Availability:
Available from INIS in electronic form
Submitting Site:
FRN
Size:
207 pages
Announcement Date:
Nov 23, 2005

Citation Formats

Chivot, J J. Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial. France: N. p., 1967. Web.
Chivot, J J. Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial. France.
Chivot, J J. 1967. "Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial." France.
@misc{etde_20653430,
title = {Radio-active colloids in the functional exploration of the reticulo-endothelium system; Les colloides radioactifs dans l'exploration fonctionnelle du systeme reticulo-endothelial}
author = {Chivot, J J}
abstractNote = {A historical review describes the reticulo-endothelial system (R.E.S.) and aims at defining it and at explaining its operation. The methods used for its examination and the colloids utilized are considered. The author has been led to prepare a special type of colloid: an albuminous complex containing radio-iodine, 'C.A. {sup 131}I', whose method of preparation and physical and biological examination are described. A human albumin, having a known optical density in solution, is heated until a change in the optical density indicates that an aggregation of the proteinic molecules has occurred. The denatured protein is iodated with {sup 131}I. Electrophoretic, ultracentrifuge and autoradiographic controls are then carried out. This atoxic and metabolisable preparation of biological origin is compared with the better defined colloidal gold which serves as reference. C.A.{sup 131}I is injected into mice. It is shown by radioactivity measurements, auto-radiographies on sections of the whole animal, and anthropo-gamma-metric detections that a high concentration occurs in the S.R.E. of the liver. These static results are only of limited importance however compared to those obtained from an in vivo study of the phenomenon. The author records the changes in the radioactivity of the blood derived from the carotid artery using a well-scintillator. He obtains directly a curve of the radioactivity of blood having a decreasing exponential form; the mathematical expression describing this curve is given. The biological half-life T 1/2 of the colloid in the blood is a measure of its phagocytosis by the S.R.E. cells. A supplementary check is provided by the direct recording of the hepatic activity using a suitably collimated exterior detector. A curve of increasing-exponential form is obtained and its parameters are corollary to the preceding curve. These tests carried out on guinea-pigs and rats make it possible to give to the S.R.E. a phagocytic index which is characteristic of its state of activity. The phagocytic index K depends also on the diameter of the colloidal particles injected and can be used as biological test for a colloid: this is the percentage of injected colloid which is retained by the S.R.E. per unit time. This index depends on anaesthetics, on the state of inflammation of the organism and on chemical mediators such as histamine. (author) [French] Un rappel historique met en lumiere le systeme reticulo-endothelial S.R.E. et tente d'en preciser l'identite et le fonctionnememt. Les methodes de son exploration et les colloides utilises sont envisages. L'auteur est amene a fabriquer un colloide d'un type particulier: un complexe albuminique radioiode le 'C.A. {sup 131}I' dont il decrit les etapes de la preparation et des essais tant physiques que biologiques. Une albumine humaine, presentant en solution une densite optique definie est portee a la chaleur jusqu'a modification de la densite optique, temoin d'une agregation des molecules proteiques. La proteine denaturee est iodee par {sup 131}I. Des controles d'electrophorese, d'ultracentrifugation et d'autoradiographie sont effectues. Cette preparation d'origine biologique atoxique et metabolisable sera comparee a l'or colloidal mieux defini qui servira de reference. Le C.A.{sup 131}I est injecte a la souris. Des mesures de radioactivite, des autoradiographies de coupe de l'animal entier, des detections de l'anthropogammametre mettent en evidence une concentration importante au niveau du S.R.E. du foie. Mais ces resultats statiques ont une portee limitee en regard de l'exploitation d'une etude cinetique in vivo du phenomene. L'auteur enregistre les variations de la radioactivite du sang derive de la carotide dans un scintillateur a puits. Il obtient directement une courbe de radioactivite sanguine d'allure exponentielle decroissante dont il definit la formule mathematique. La periode biologique T 1/2 du colloide dans le sang est le reflet de sa phagocytose par les cellules du S.R.E. Une verification supplementaire est apportee par l'enregistrement direct de la radioactivite hepatique a l'aide d'un detecteur externe convenablement collimate. Une courbe d'allure exponentielle croissante est obtenue dont les parametres sont corollaires de la courbe precedente. Ces etudes cinetiques effectuees chez le Cobaye et chez le Rat permettent d'attribuer au S.R.F.. un index phagocytaire reflet de son etat d'activite. L'index phagocytaire K depend egalement de la taille des particules colloidales injectees et peut servir de test biologique pour un colloide: c'est le pourcentage de colloide injecte retenu par les cellules du systeme reticulo-endothelial dans l'unite de temps. Cet index est sous la dependance des anesthesiques, de l'etat d'inflammation de l'organisme, et de mediateurs chimiques telle que l'histamine. (auteur)}
place = {France}
year = {1967}
month = {Mar}
}