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Biodegradability of fuel-ethers in environment; Biodegradabilite des ethers-carburants dans l'environnement

Abstract

Fuel ethers (methyl tert-butyl ether or MTBE, ethyl tert-butyl ether or ETBE and tert-amyl methyl ether or TAME have been used as gasoline additives since about twenty years in order to meet the requirements for the octane index and to limit the polluting emission in exhaust pipe gas (unburnt hydrocarbons and carbon monoxide). The high water solubility and the poor biodegradability of these compounds make them pollutants frequently encountered in aquifers. The present manuscript summarizes the knowledge concerning the biodegradability of fuel ethers obtained both at IFP and during collaborations with the Pasteur Institute (Paris), the Biotechnology Research Institute (Montreal, Canada) and the Center for Environmental Biotechnology (University of Tennessee, USA). Rhodococcus ruber IFP 2001 and Mycobacterium austroafricanum IFP 2012, two microorganisms isolated at IFP for their ability to grow, respectively, on ETBE and MTBE, were studied in order to determine the intermediates produced during MTBE and ETBE biodegradation and the enzymes required for each biodegradation step, thus allowing us to propose MTBE and ETBE catabolic pathways. A proteomic approach, from the protein induced during the degradation of ETBE or MTBE to the genes encoding these different enzymes, was carried out. The isolation of such genes is required:1) to use  More>>
Publication Date:
Apr 01, 2005
Product Type:
Thesis/Dissertation
Report Number:
IFP-58654
Reference Number:
RN05136583; TVI: 0510
Resource Relation:
Other Information: TH: These UFR sciences de la vie; 315 refs.
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; POLLUTION; TOXICITY; LEGISLATION; EPIDEMIOLOGY; AEROBIC CONDITIONS; IN VITRO; ENZYMES; BIOCHEMICAL REACTION KINETICS; ENZYME INHIBITORS; BUTYL ETHER; ANAEROBIC CONDITIONS; BIOREMEDIATION; XENOBIOTICS; RESEARCH PROGRAMS; FUEL ADDITIVES; SOLUBILITY; MYCOBACTERIUM; RHODOCOCCUS
OSTI ID:
20648634
Research Organizations:
Institut Francais du Petrole (IFP), 92 - Rueil-Malmaison (France); Universite Pierre et Marie Curie Paris-6, 75 - Paris (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
TRN: FR0503384
Availability:
Commercial reproduction prohibited; OSTI as DE20648634
Submitting Site:
FR
Size:
243 pages
Announcement Date:

Citation Formats

Fayolle-Guichard, F. Biodegradability of fuel-ethers in environment; Biodegradabilite des ethers-carburants dans l'environnement. France: N. p., 2005. Web.
Fayolle-Guichard, F. Biodegradability of fuel-ethers in environment; Biodegradabilite des ethers-carburants dans l'environnement. France.
Fayolle-Guichard, F. 2005. "Biodegradability of fuel-ethers in environment; Biodegradabilite des ethers-carburants dans l'environnement." France.
@misc{etde_20648634,
title = {Biodegradability of fuel-ethers in environment; Biodegradabilite des ethers-carburants dans l'environnement}
author = {Fayolle-Guichard, F}
abstractNote = {Fuel ethers (methyl tert-butyl ether or MTBE, ethyl tert-butyl ether or ETBE and tert-amyl methyl ether or TAME have been used as gasoline additives since about twenty years in order to meet the requirements for the octane index and to limit the polluting emission in exhaust pipe gas (unburnt hydrocarbons and carbon monoxide). The high water solubility and the poor biodegradability of these compounds make them pollutants frequently encountered in aquifers. The present manuscript summarizes the knowledge concerning the biodegradability of fuel ethers obtained both at IFP and during collaborations with the Pasteur Institute (Paris), the Biotechnology Research Institute (Montreal, Canada) and the Center for Environmental Biotechnology (University of Tennessee, USA). Rhodococcus ruber IFP 2001 and Mycobacterium austroafricanum IFP 2012, two microorganisms isolated at IFP for their ability to grow, respectively, on ETBE and MTBE, were studied in order to determine the intermediates produced during MTBE and ETBE biodegradation and the enzymes required for each biodegradation step, thus allowing us to propose MTBE and ETBE catabolic pathways. A proteomic approach, from the protein induced during the degradation of ETBE or MTBE to the genes encoding these different enzymes, was carried out. The isolation of such genes is required:1) to use them for help in determining the bio-remediation capacities in polluted aquifers (DNA micro-arrays), 2) to monitor the microorganisms isolated for their degradative capacities during bio-remediation processes (fluorescent in situ hybridization or FISH) and 3) to create new tools for the detection and the quantification of ETBE or MTBE in contaminated aquifers (bio-sensor). The manuscript also describes the different ways for the adaptation of microorganisms to the presence of a xenobiotic compound. (author)}
place = {France}
year = {2005}
month = {Apr}
}