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Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B

Abstract

Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-{kappa}B). The activation of NF-{kappa}B has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-{kappa}B in A549 cells. Electromobility shift assays show increased NF-{kappa}B complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-{kappa}B depends on the C-terminus of the RSV M2-1 protein, as shown by NF-{kappa}B-induced gene expression of a reporter gene construct.
Authors:
Reimers, Kerstin; [1]  Buchholz, Katja; [2]  Werchau, Hermann [2] 
  1. Klinik fuer Plastische, Hand-und Wiederherstellungschirurgie, Podbielskistrasse 380, D-30659 Hannover (Germany)
  2. Institut fuer Medizinische Mikrobiologie, Otto-von-Guericke-Universitaet Magdeburg, Leipzigerstrasse 44, D-39120 Magdeburg (Germany)
Publication Date:
Jan 20, 2005
Product Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 331; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2004.10.031; PII: S0042-6822(04)00682-8; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; GENES; INFECTIOUS DISEASES; LYMPHOKINES; RESPIRATORY SYSTEM DISEASES; VIRUSES
OSTI ID:
20637195
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0042-6822; VIRLAX; TRN: US05R2653080527
Submitting Site:
INIS
Size:
page(s) 260-268
Announcement Date:
Nov 03, 2005

Citation Formats

Reimers, Kerstin, Buchholz, Katja, and Werchau, Hermann. Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B. United States: N. p., 2005. Web. doi:10.1016/j.virol.2004.10.031.
Reimers, Kerstin, Buchholz, Katja, & Werchau, Hermann. Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B. United States. doi:10.1016/j.virol.2004.10.031.
Reimers, Kerstin, Buchholz, Katja, and Werchau, Hermann. 2005. "Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B." United States. doi:10.1016/j.virol.2004.10.031. https://www.osti.gov/servlets/purl/10.1016/j.virol.2004.10.031.
@misc{etde_20637195,
title = {Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B}
author = {Reimers, Kerstin, Buchholz, Katja, and Werchau, Hermann}
abstractNote = {Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-{kappa}B). The activation of NF-{kappa}B has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-{kappa}B in A549 cells. Electromobility shift assays show increased NF-{kappa}B complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-{kappa}B depends on the C-terminus of the RSV M2-1 protein, as shown by NF-{kappa}B-induced gene expression of a reporter gene construct.}
doi = {10.1016/j.virol.2004.10.031}
journal = {Virology}
issue = {2}
volume = {331}
place = {United States}
year = {2005}
month = {Jan}
}