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Apoptosis induction in human lymphocytes after in vitro exposure to cobalt/hard metal compounds

Abstract

Full text: An increased risk of lung cancer is associated with occupational exposure to mixtures of cobalt metal (Co) and tungsten carbide (WC) particles, but apparently not when exposure is to cobalt alone. The mechanism for this increased cancer risk is not fully understood. The evaluation of the in vitro genotoxic effects in lymphocytes exposed to varying cobalt species demonstrated that the WC-Co hard metal mixture is more genotoxic (DNA damage, chromosome/genome mutations) than metallic Co alone. WC alone was not genotoxic. Thus, WC-Co represents a specific (geno)toxic entity. In order to assess the survival of human lymphocytes after in vitro exposure to metallic Co, CoCl{sub 2}, WC and the WC-Co mixture, two apoptosis/necrosis detection methods were applied (annexin V staining and flow cytometry). Annexin-V staining of early apoptotic cells demonstrated a dose- and time dependent induction of apoptosis by metallic Co, CoCl{sub 2}, WC and the WC-Co mixture. The time course of the process varied according to the metal species tested. Metallic Co and CoCl{sub 2} caused a gradually increasing frequency of apoptotic cells with time (up to 24 h). WC-induced apoptosis displayed a typical 6 hour peak, which was not the case for the WC-Co mixture or for  More>>
Authors:
Boeck, M de; Decordier, I; Lombaert, N; Cundari, E; Kirsch-Volders, M; [1]  Lison, D [2] 
  1. Vrije Universiteit Brussel, Laboratorium voor Cellulaire Genetica, Brussel (Belgium)
  2. Universite catholique de Louvain, Unite de Toxicologie industrielle et Medecine du Travail, Bruxelles (Belgium)
Publication Date:
Jul 01, 2001
Product Type:
Conference
Resource Relation:
Conference: 31. annual meeting of the European Environmental Mutagen Society (EEMS), Ghent (Belgium), 1-5 Sep 2001; Related Information: In: Genetic susceptibility at low dose exposure. Abstracts, 189 pages.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; APOPTOSIS; CERMETS; CHROMOSOMES; COBALT; COBALT CHLORIDES; DNA DAMAGES; GENOME MUTATIONS; HEALTH HAZARDS; IN VITRO; IN VIVO; IODIDES; LUNGS; LYMPHOCYTES; NECROSIS; NEOPLASMS; OCCUPATIONAL EXPOSURE; RADIATION DOSES; TIME DEPENDENCE
OSTI ID:
20634962
Research Organizations:
European Environmental Mutagen Society, Ghent (Belgium); Belgian Environmental Mutagen Society, Ghent (Belgium)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA05C0790078190
Availability:
Available in abstract form only at: http://www.swan.ac.uk/cget/ejgt/issues.htm; Full text entered in this record
Submitting Site:
INIS
Size:
page(s) 173
Announcement Date:
Nov 14, 2005

Citation Formats

Boeck, M de, Decordier, I, Lombaert, N, Cundari, E, Kirsch-Volders, M, and Lison, D. Apoptosis induction in human lymphocytes after in vitro exposure to cobalt/hard metal compounds. IAEA: N. p., 2001. Web.
Boeck, M de, Decordier, I, Lombaert, N, Cundari, E, Kirsch-Volders, M, & Lison, D. Apoptosis induction in human lymphocytes after in vitro exposure to cobalt/hard metal compounds. IAEA.
Boeck, M de, Decordier, I, Lombaert, N, Cundari, E, Kirsch-Volders, M, and Lison, D. 2001. "Apoptosis induction in human lymphocytes after in vitro exposure to cobalt/hard metal compounds." IAEA.
@misc{etde_20634962,
title = {Apoptosis induction in human lymphocytes after in vitro exposure to cobalt/hard metal compounds}
author = {Boeck, M de, Decordier, I, Lombaert, N, Cundari, E, Kirsch-Volders, M, and Lison, D}
abstractNote = {Full text: An increased risk of lung cancer is associated with occupational exposure to mixtures of cobalt metal (Co) and tungsten carbide (WC) particles, but apparently not when exposure is to cobalt alone. The mechanism for this increased cancer risk is not fully understood. The evaluation of the in vitro genotoxic effects in lymphocytes exposed to varying cobalt species demonstrated that the WC-Co hard metal mixture is more genotoxic (DNA damage, chromosome/genome mutations) than metallic Co alone. WC alone was not genotoxic. Thus, WC-Co represents a specific (geno)toxic entity. In order to assess the survival of human lymphocytes after in vitro exposure to metallic Co, CoCl{sub 2}, WC and the WC-Co mixture, two apoptosis/necrosis detection methods were applied (annexin V staining and flow cytometry). Annexin-V staining of early apoptotic cells demonstrated a dose- and time dependent induction of apoptosis by metallic Co, CoCl{sub 2}, WC and the WC-Co mixture. The time course of the process varied according to the metal species tested. Metallic Co and CoCl{sub 2} caused a gradually increasing frequency of apoptotic cells with time (up to 24 h). WC-induced apoptosis displayed a typical 6 hour peak, which was not the case for the WC-Co mixture or for Co. Apoptosis induction by the WC-Co mixture was intermediate between that induced by Co and WC separately. Analysis of propidium iodide stained cells by flow cytometry was performed as a later marker for apoptosis induction. Preliminary data indicate similar tendencies of apoptosis induction as those detected by annexin-V. Identification of the apoptotic pathway triggered by the metal compounds was studied by inhibition of the ceramide-apoptosis pathway by fumonisin causing reduction of apoptosis induction for all compounds, but strongest after 6 hour exposure to WC. The use of specific caspase inhibitors will allow to further elucidate the different pathways involved. The current data demonstrating in vitro the apoptosis induction by metal compounds, in addition to their in vitro genotoxic activity may help to explain their in vivo carcinogenicity in humans. (author)}
place = {IAEA}
year = {2001}
month = {Jul}
}