You need JavaScript to view this

Isolation and characterisation of hsp70 genes in Bactrocera oleae

Abstract

Full text: Bactrocera oleae is an insect pest of great economical importance, causing annually great losses to the olive fruit crops. Molecular studies of the insect can significantly contribute to the efforts of its biological control. The present report is part of an ongoing effort of analysing and mapping the B. oleae genome and is focussed on the analysis of the hsp70 gene. The hsp70 gene, a member of the Hsp70 family, presents high levels of conservation and plays a crucial role during heat-shock response. In Drosophila and other Diptera species multiple hsp70 gene copies have been identified, while its promoter has been widely used for the expression of several genes in insect transformation systems. Three {lambda} clones bearing hsp70 sequences were isolated by screening a genomic library of the standard laboratory B. oleae population with parts of a Ceratitis capitata hsp70 copy. All three clones map cytogenetically on the major heat shock puff located at region 96 of the B. oleae VR salivary gland polytene chromosome arm. Restriction mapping of the three clones, as well as genomic Southern hybridisation indicated the presence of five putative hsp70 genes in B. oleae. Four of these genes are arranged within an approximately  More>>
Authors:
Drosopoulou, E; [1]  Chrysopoulou, E; Nikita, V; Mavragani-Tsipidou, P [1] 
  1. Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki (Greece)
Publication Date:
Jul 01, 2005
Product Type:
Conference
Report Number:
IAEA-CN-131; IAEA-CN-131/10P
Resource Relation:
Conference: FAO/IAEA international conference on area-wide control of insect pests: Integrating the sterile insect and related nuclear and other techniques, Vienna (Austria), 9-13 May 2005; Related Information: In: FAO/IAEA international conference on area-wide control of insect pests: Integrating the sterile insect and related nuclear and other techniques. Book of extended synopses, 386 pages.
Subject:
60 APPLIED LIFE SCIENCES; CERATITIS CAPITATA; CHROMOSOMES; CROPS; DROSOPHILA; GENES; HYBRIDIZATION; OLIVES; SALIVARY GLANDS
OSTI ID:
20633133
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA0501523076028
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 309
Announcement Date:
Oct 31, 2005

Citation Formats

Drosopoulou, E, Chrysopoulou, E, Nikita, V, and Mavragani-Tsipidou, P. Isolation and characterisation of hsp70 genes in Bactrocera oleae. IAEA: N. p., 2005. Web.
Drosopoulou, E, Chrysopoulou, E, Nikita, V, & Mavragani-Tsipidou, P. Isolation and characterisation of hsp70 genes in Bactrocera oleae. IAEA.
Drosopoulou, E, Chrysopoulou, E, Nikita, V, and Mavragani-Tsipidou, P. 2005. "Isolation and characterisation of hsp70 genes in Bactrocera oleae." IAEA.
@misc{etde_20633133,
title = {Isolation and characterisation of hsp70 genes in Bactrocera oleae}
author = {Drosopoulou, E, Chrysopoulou, E, Nikita, V, and Mavragani-Tsipidou, P}
abstractNote = {Full text: Bactrocera oleae is an insect pest of great economical importance, causing annually great losses to the olive fruit crops. Molecular studies of the insect can significantly contribute to the efforts of its biological control. The present report is part of an ongoing effort of analysing and mapping the B. oleae genome and is focussed on the analysis of the hsp70 gene. The hsp70 gene, a member of the Hsp70 family, presents high levels of conservation and plays a crucial role during heat-shock response. In Drosophila and other Diptera species multiple hsp70 gene copies have been identified, while its promoter has been widely used for the expression of several genes in insect transformation systems. Three {lambda} clones bearing hsp70 sequences were isolated by screening a genomic library of the standard laboratory B. oleae population with parts of a Ceratitis capitata hsp70 copy. All three clones map cytogenetically on the major heat shock puff located at region 96 of the B. oleae VR salivary gland polytene chromosome arm. Restriction mapping of the three clones, as well as genomic Southern hybridisation indicated the presence of five putative hsp70 genes in B. oleae. Four of these genes are arranged within an approximately 25 kb long genomic region presenting parallel orientation, as revealed by differential hybridisation of the restriction fragments to the 5'nd 3' C. capitata probes. Sequence analysis of one of these genes revealed a single open reading frame bearing very high homology to an hsp70 gene copy of C. capitata. A TATA box, as well as a putative cap signal has been found within a 200 bp long genomic region upstream from the coding start (ATG). Furthermore, close upstream from the TATA box three characteristic heat shock elements have been identified in the promoter of this gene copy, suggesting that it represents a heat-inducible member of the Hsp70 gene family. (author)}
place = {IAEA}
year = {2005}
month = {Jul}
}