Abstract
The co-immobilization of cyclohexanone monooxygenase (CHMO) and glucose-6-phosphate dehydrogenase (G6PDH) was optimized by completely coating, via covalent immobilization, the surface aldehyde groups of porous agarose (glyoxyl-agarose) with amine groups of polyethylenimine (PEI). The highest immobilization efficiency ({approx}87%) (activity of enzyme per amount of immobilized enzyme) was obtained with a CHMO/G6PDH ratio 2:1. The effects of different ratios of the support to the amount of enzymes (CHMO:G6PDH=2:1), the optimum incubation pH and the incubation time on the enzymatic activity of the enzymes were determined and found to be 5:1, 8.5 and 30 min, respectively. Subjecting the co-immobilized enzymes to doses of {gamma}-radiation (5-100 kGy) resulted in complete loss in the activity of the free enzymes at a dose of 40 kGy, while the co-immobilized ones showed relatively high resistance to {gamma}-radiation up to a dose of 50 kGy.
Atia, K S
[1]
- Atomic Energy Authority, Nuclear Research Center, Abo-Zabal, P.O. Box 13759, Cairo (Egypt)
Citation Formats
Atia, K S.
Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using {gamma} irradiation to use in biotechnological processes.
United Kingdom: N. p.,
2005.
Web.
doi:10.1016/j.radphyschem.2004.07.005.
Atia, K S.
Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using {gamma} irradiation to use in biotechnological processes.
United Kingdom.
https://doi.org/10.1016/j.radphyschem.2004.07.005
Atia, K S.
2005.
"Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using {gamma} irradiation to use in biotechnological processes."
United Kingdom.
https://doi.org/10.1016/j.radphyschem.2004.07.005.
@misc{etde_20620280,
title = {Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using {gamma} irradiation to use in biotechnological processes}
author = {Atia, K S}
abstractNote = {The co-immobilization of cyclohexanone monooxygenase (CHMO) and glucose-6-phosphate dehydrogenase (G6PDH) was optimized by completely coating, via covalent immobilization, the surface aldehyde groups of porous agarose (glyoxyl-agarose) with amine groups of polyethylenimine (PEI). The highest immobilization efficiency ({approx}87%) (activity of enzyme per amount of immobilized enzyme) was obtained with a CHMO/G6PDH ratio 2:1. The effects of different ratios of the support to the amount of enzymes (CHMO:G6PDH=2:1), the optimum incubation pH and the incubation time on the enzymatic activity of the enzymes were determined and found to be 5:1, 8.5 and 30 min, respectively. Subjecting the co-immobilized enzymes to doses of {gamma}-radiation (5-100 kGy) resulted in complete loss in the activity of the free enzymes at a dose of 40 kGy, while the co-immobilized ones showed relatively high resistance to {gamma}-radiation up to a dose of 50 kGy.}
doi = {10.1016/j.radphyschem.2004.07.005}
journal = []
issue = {2}
volume = {73}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Jun}
}
title = {Co-immobilization of cyclohexanone monooxygenase and glucose-6-phosphate dehydrogenase onto polyethylenimine-porous agarose polymeric composite using {gamma} irradiation to use in biotechnological processes}
author = {Atia, K S}
abstractNote = {The co-immobilization of cyclohexanone monooxygenase (CHMO) and glucose-6-phosphate dehydrogenase (G6PDH) was optimized by completely coating, via covalent immobilization, the surface aldehyde groups of porous agarose (glyoxyl-agarose) with amine groups of polyethylenimine (PEI). The highest immobilization efficiency ({approx}87%) (activity of enzyme per amount of immobilized enzyme) was obtained with a CHMO/G6PDH ratio 2:1. The effects of different ratios of the support to the amount of enzymes (CHMO:G6PDH=2:1), the optimum incubation pH and the incubation time on the enzymatic activity of the enzymes were determined and found to be 5:1, 8.5 and 30 min, respectively. Subjecting the co-immobilized enzymes to doses of {gamma}-radiation (5-100 kGy) resulted in complete loss in the activity of the free enzymes at a dose of 40 kGy, while the co-immobilized ones showed relatively high resistance to {gamma}-radiation up to a dose of 50 kGy.}
doi = {10.1016/j.radphyschem.2004.07.005}
journal = []
issue = {2}
volume = {73}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Jun}
}