Abstract
Irradiation of proteins in diluted liquid aqueous solutions produces cleavages and polymerizations of the peptidic chains. In frozen solutions, fragmentation is observed but polymerization products are absent. Loss of activity occurs in both cases. In the solid state, yields of fragmentation do not vary with the quantity of water. The use of scavengers indicates that hydroxyl radical does not contribute significantly to fragmentation and to inactivation in the solid state. Electrons within the water molecules closely associated with the protein are involved in the processes leading to protein fragmentation.
Audette-Stuart, Marilyne;
[1]
Houee-Levin, Chantal;
[2]
Potier, Michel
[3]
- Atomic Energy of Canada Limited, CANDU Life Sciences Center, Chalk River Laboratories, Chalk River Ont., K0J 1J0 (Canada)
- Laboratoire de Chimie Physique, UMR-8000 CNRS-Universite Paris XI, Centre Universitaire, F-91405 Orsay Cedex (France)
- Service de genetique medicale, Hopital Sainte-Justine, Universite de Montreal, Montreal Que., H3 T 1C5 (Canada)
Citation Formats
Audette-Stuart, Marilyne, Houee-Levin, Chantal, and Potier, Michel.
Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons.
United Kingdom: N. p.,
2005.
Web.
doi:10.1016/j.radphyschem.2003.12.060.
Audette-Stuart, Marilyne, Houee-Levin, Chantal, & Potier, Michel.
Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons.
United Kingdom.
https://doi.org/10.1016/j.radphyschem.2003.12.060
Audette-Stuart, Marilyne, Houee-Levin, Chantal, and Potier, Michel.
2005.
"Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons."
United Kingdom.
https://doi.org/10.1016/j.radphyschem.2003.12.060.
@misc{etde_20620255,
title = {Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons}
author = {Audette-Stuart, Marilyne, Houee-Levin, Chantal, and Potier, Michel}
abstractNote = {Irradiation of proteins in diluted liquid aqueous solutions produces cleavages and polymerizations of the peptidic chains. In frozen solutions, fragmentation is observed but polymerization products are absent. Loss of activity occurs in both cases. In the solid state, yields of fragmentation do not vary with the quantity of water. The use of scavengers indicates that hydroxyl radical does not contribute significantly to fragmentation and to inactivation in the solid state. Electrons within the water molecules closely associated with the protein are involved in the processes leading to protein fragmentation.}
doi = {10.1016/j.radphyschem.2003.12.060}
journal = []
issue = {2-3}
volume = {72}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Feb}
}
title = {Radiation-induced protein fragmentation and inactivation in liquid and solid aqueous solutions. Role of OH and electrons}
author = {Audette-Stuart, Marilyne, Houee-Levin, Chantal, and Potier, Michel}
abstractNote = {Irradiation of proteins in diluted liquid aqueous solutions produces cleavages and polymerizations of the peptidic chains. In frozen solutions, fragmentation is observed but polymerization products are absent. Loss of activity occurs in both cases. In the solid state, yields of fragmentation do not vary with the quantity of water. The use of scavengers indicates that hydroxyl radical does not contribute significantly to fragmentation and to inactivation in the solid state. Electrons within the water molecules closely associated with the protein are involved in the processes leading to protein fragmentation.}
doi = {10.1016/j.radphyschem.2003.12.060}
journal = []
issue = {2-3}
volume = {72}
journal type = {AC}
place = {United Kingdom}
year = {2005}
month = {Feb}
}