Abstract
Survivin is critically involved in mitosis and when overexpressed enhances the activity of the Aurora B kinase, a serine-threonine kinase belonging to the family of oncogenic Aurora/IpI1p-related kinases. Both proteins interact with Ras GTPase-activating protein suggesting an impact on the Ras pathway. This study aimed at defining the role of survivin in proliferation and potential transformation of cells. When survivin was overexpressed in normal human lung fibroblasts, the characteristic track lanes of fibroblasts were disturbed and the rate of cell proliferation was increased. An enhanced level of p21{sup ras} mRNA and protein expression and concomitant rise in levels of activated p21{sup ras} were observed. Despite increased proliferation cell survival remained dependent on serum and cells were not able to form colonies in soft agar assays. These data suggest that overexpression of survivin increases cell growth but, despite the increase in active p21{sup ras}, is not sufficient to transform primary cells. Yet, in addition to its anti-apoptotic function it might contribute to the accelerated growth of tumour cells by increasing p21{sup ras} activity.
Temme, Achim;
[1]
Diestelkoetter-Bachert, Petra;
[1]
Schmitz, Marc;
[1]
Morgenroth, Agnieszka;
[1]
Weigle, Bernd;
[1]
Rieger, Michael A;
[1]
Kiessling, Andrea;
[1]
Rieber, E Peter
[1]
- Institute of Immunology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Fetscherstrasse 74, 01307 Dresden (Germany)
Citation Formats
Temme, Achim, Diestelkoetter-Bachert, Petra, Schmitz, Marc, Morgenroth, Agnieszka, Weigle, Bernd, Rieger, Michael A, Kiessling, Andrea, and Rieber, E Peter.
Increased p21{sup ras} activity in human fibroblasts transduced with survivin enhances cell proliferation.
United States: N. p.,
2005.
Web.
doi:10.1016/j.bbrc.2004.12.075.
Temme, Achim, Diestelkoetter-Bachert, Petra, Schmitz, Marc, Morgenroth, Agnieszka, Weigle, Bernd, Rieger, Michael A, Kiessling, Andrea, & Rieber, E Peter.
Increased p21{sup ras} activity in human fibroblasts transduced with survivin enhances cell proliferation.
United States.
https://doi.org/10.1016/j.bbrc.2004.12.075
Temme, Achim, Diestelkoetter-Bachert, Petra, Schmitz, Marc, Morgenroth, Agnieszka, Weigle, Bernd, Rieger, Michael A, Kiessling, Andrea, and Rieber, E Peter.
2005.
"Increased p21{sup ras} activity in human fibroblasts transduced with survivin enhances cell proliferation."
United States.
https://doi.org/10.1016/j.bbrc.2004.12.075.
@misc{etde_20619390,
title = {Increased p21{sup ras} activity in human fibroblasts transduced with survivin enhances cell proliferation}
author = {Temme, Achim, Diestelkoetter-Bachert, Petra, Schmitz, Marc, Morgenroth, Agnieszka, Weigle, Bernd, Rieger, Michael A, Kiessling, Andrea, and Rieber, E Peter}
abstractNote = {Survivin is critically involved in mitosis and when overexpressed enhances the activity of the Aurora B kinase, a serine-threonine kinase belonging to the family of oncogenic Aurora/IpI1p-related kinases. Both proteins interact with Ras GTPase-activating protein suggesting an impact on the Ras pathway. This study aimed at defining the role of survivin in proliferation and potential transformation of cells. When survivin was overexpressed in normal human lung fibroblasts, the characteristic track lanes of fibroblasts were disturbed and the rate of cell proliferation was increased. An enhanced level of p21{sup ras} mRNA and protein expression and concomitant rise in levels of activated p21{sup ras} were observed. Despite increased proliferation cell survival remained dependent on serum and cells were not able to form colonies in soft agar assays. These data suggest that overexpression of survivin increases cell growth but, despite the increase in active p21{sup ras}, is not sufficient to transform primary cells. Yet, in addition to its anti-apoptotic function it might contribute to the accelerated growth of tumour cells by increasing p21{sup ras} activity.}
doi = {10.1016/j.bbrc.2004.12.075}
journal = []
issue = {3}
volume = {327}
journal type = {AC}
place = {United States}
year = {2005}
month = {Feb}
}
title = {Increased p21{sup ras} activity in human fibroblasts transduced with survivin enhances cell proliferation}
author = {Temme, Achim, Diestelkoetter-Bachert, Petra, Schmitz, Marc, Morgenroth, Agnieszka, Weigle, Bernd, Rieger, Michael A, Kiessling, Andrea, and Rieber, E Peter}
abstractNote = {Survivin is critically involved in mitosis and when overexpressed enhances the activity of the Aurora B kinase, a serine-threonine kinase belonging to the family of oncogenic Aurora/IpI1p-related kinases. Both proteins interact with Ras GTPase-activating protein suggesting an impact on the Ras pathway. This study aimed at defining the role of survivin in proliferation and potential transformation of cells. When survivin was overexpressed in normal human lung fibroblasts, the characteristic track lanes of fibroblasts were disturbed and the rate of cell proliferation was increased. An enhanced level of p21{sup ras} mRNA and protein expression and concomitant rise in levels of activated p21{sup ras} were observed. Despite increased proliferation cell survival remained dependent on serum and cells were not able to form colonies in soft agar assays. These data suggest that overexpression of survivin increases cell growth but, despite the increase in active p21{sup ras}, is not sufficient to transform primary cells. Yet, in addition to its anti-apoptotic function it might contribute to the accelerated growth of tumour cells by increasing p21{sup ras} activity.}
doi = {10.1016/j.bbrc.2004.12.075}
journal = []
issue = {3}
volume = {327}
journal type = {AC}
place = {United States}
year = {2005}
month = {Feb}
}