Abstract
A new method for the simultaneous determination of heavy-metal ions in tobacco and tobacco additive by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The tobacco and tobacco additive samples were digested by microwave digestion. The lead, cadmium, mercury, nickel, copper, and tin ions in the digested samples were pre-column derivated with tetra-(4-amino-phenyl)-porphyrin (T4-APP) to form color chelates; the Hg-T4-APP, Cd-T4-APP, Pb-T4-APP, Ni-T4-APP, Cu-T4-APP, and Sn-T4-APP chelates were then enriched by solid-phase extraction with C{sub 18} disks and the retained chelates were eluted from the disks using tetrahydrofuran. The chelates were separated on a Waters Xterra{sup TM} RP{sub 18} column by gradient using methanol (containing 0.05 mol/L pyrrolidine-acetic acid buffer salt, pH = 10.0) and acetone (containing 0.05 mol/l pyrrolidine-acetic acid buffer salt, pH = 10.0) as a mobile phase at a flow rate of 0.5 ml/min and detected with a photodiode array detector in the range 350-600 nm. The detection limits of lead, cadmium, mercury, nickel, copper, and tin were 5, 4, 2.5, 5, 8, and 4 ng/L, respectively, in the original samples. The method was applied to the determination of lead, cadmium, mercury, nickel, copper, and tin in tobacco and tobacco additive with good results.
Guan'yu Yang, G;
Li, Zh;
Shi, K Kh;
[1]
Vang, Yu
[2]
- Klyuchevaya Laboratoriya Khimii i Inzhenerii Akademii Nauk po Issledovaniyam Tabaka v Yun'nane, Kun'min (China)
- KAN, Dalyan'skij Inst. Khimicheskoj Fiziki, Dalyan' (China)
Citation Formats
Guan'yu Yang, G, Li, Zh, Shi, K Kh, and Vang, Yu.
Study on the determination of heavy-metal ions in tobacco and tobacco additives by microwave digestion and HPLC with PAD Detection; Opredelenie ionov tyazhelykh metallov v tabake i v dobavkakh k tabaku s ispol'zovaniem mikrovolnovogo razlozheniya i vysokoehffektivnoj zhidkostnoj khromatografii.
Russian Federation: N. p.,
2005.
Web.
Guan'yu Yang, G, Li, Zh, Shi, K Kh, & Vang, Yu.
Study on the determination of heavy-metal ions in tobacco and tobacco additives by microwave digestion and HPLC with PAD Detection; Opredelenie ionov tyazhelykh metallov v tabake i v dobavkakh k tabaku s ispol'zovaniem mikrovolnovogo razlozheniya i vysokoehffektivnoj zhidkostnoj khromatografii.
Russian Federation.
Guan'yu Yang, G, Li, Zh, Shi, K Kh, and Vang, Yu.
2005.
"Study on the determination of heavy-metal ions in tobacco and tobacco additives by microwave digestion and HPLC with PAD Detection; Opredelenie ionov tyazhelykh metallov v tabake i v dobavkakh k tabaku s ispol'zovaniem mikrovolnovogo razlozheniya i vysokoehffektivnoj zhidkostnoj khromatografii."
Russian Federation.
@misc{etde_20617486,
title = {Study on the determination of heavy-metal ions in tobacco and tobacco additives by microwave digestion and HPLC with PAD Detection; Opredelenie ionov tyazhelykh metallov v tabake i v dobavkakh k tabaku s ispol'zovaniem mikrovolnovogo razlozheniya i vysokoehffektivnoj zhidkostnoj khromatografii}
author = {Guan'yu Yang, G, Li, Zh, Shi, K Kh, and Vang, Yu}
abstractNote = {A new method for the simultaneous determination of heavy-metal ions in tobacco and tobacco additive by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The tobacco and tobacco additive samples were digested by microwave digestion. The lead, cadmium, mercury, nickel, copper, and tin ions in the digested samples were pre-column derivated with tetra-(4-amino-phenyl)-porphyrin (T4-APP) to form color chelates; the Hg-T4-APP, Cd-T4-APP, Pb-T4-APP, Ni-T4-APP, Cu-T4-APP, and Sn-T4-APP chelates were then enriched by solid-phase extraction with C{sub 18} disks and the retained chelates were eluted from the disks using tetrahydrofuran. The chelates were separated on a Waters Xterra{sup TM} RP{sub 18} column by gradient using methanol (containing 0.05 mol/L pyrrolidine-acetic acid buffer salt, pH = 10.0) and acetone (containing 0.05 mol/l pyrrolidine-acetic acid buffer salt, pH = 10.0) as a mobile phase at a flow rate of 0.5 ml/min and detected with a photodiode array detector in the range 350-600 nm. The detection limits of lead, cadmium, mercury, nickel, copper, and tin were 5, 4, 2.5, 5, 8, and 4 ng/L, respectively, in the original samples. The method was applied to the determination of lead, cadmium, mercury, nickel, copper, and tin in tobacco and tobacco additive with good results.}
journal = []
issue = {5}
volume = {60}
journal type = {AC}
place = {Russian Federation}
year = {2005}
month = {May}
}
title = {Study on the determination of heavy-metal ions in tobacco and tobacco additives by microwave digestion and HPLC with PAD Detection; Opredelenie ionov tyazhelykh metallov v tabake i v dobavkakh k tabaku s ispol'zovaniem mikrovolnovogo razlozheniya i vysokoehffektivnoj zhidkostnoj khromatografii}
author = {Guan'yu Yang, G, Li, Zh, Shi, K Kh, and Vang, Yu}
abstractNote = {A new method for the simultaneous determination of heavy-metal ions in tobacco and tobacco additive by microwave digestion and reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The tobacco and tobacco additive samples were digested by microwave digestion. The lead, cadmium, mercury, nickel, copper, and tin ions in the digested samples were pre-column derivated with tetra-(4-amino-phenyl)-porphyrin (T4-APP) to form color chelates; the Hg-T4-APP, Cd-T4-APP, Pb-T4-APP, Ni-T4-APP, Cu-T4-APP, and Sn-T4-APP chelates were then enriched by solid-phase extraction with C{sub 18} disks and the retained chelates were eluted from the disks using tetrahydrofuran. The chelates were separated on a Waters Xterra{sup TM} RP{sub 18} column by gradient using methanol (containing 0.05 mol/L pyrrolidine-acetic acid buffer salt, pH = 10.0) and acetone (containing 0.05 mol/l pyrrolidine-acetic acid buffer salt, pH = 10.0) as a mobile phase at a flow rate of 0.5 ml/min and detected with a photodiode array detector in the range 350-600 nm. The detection limits of lead, cadmium, mercury, nickel, copper, and tin were 5, 4, 2.5, 5, 8, and 4 ng/L, respectively, in the original samples. The method was applied to the determination of lead, cadmium, mercury, nickel, copper, and tin in tobacco and tobacco additive with good results.}
journal = []
issue = {5}
volume = {60}
journal type = {AC}
place = {Russian Federation}
year = {2005}
month = {May}
}