Abstract
In the past, the use of longer X-ray wavelengths ({lambda} = 1.5 A -3 A) has become quite popular in macromolecular crystallography. The main reason for this is that the anomalous signal which can be obtained from the light atoms (P, S, C, Ca, etc.), from the medium heavy atoms (I, Xe, etc) and from the very heavy atoms (U etc.) can be significantly enhanced. Therefore, data collection at longer X-ray wavelength's may lead to significant advantage for experimental phase determination. It has been shown previously, that the experimental difficulties associated with the use of these longer wavelength's, namely absorption, can be dealt with sufficiently by proper scaling and that as a consequence these longer wavelength's could be utilized on a routine basis. Nevertheless, in the case of the light atoms, the obtainable anomalous signal is still very small, therefore it is absolutely essential that the data are collected with very high accuracy. As was shown previously, if the data are collected at high redundancy, very small errors in the anomalous differences can be obtained. The combination of the three effects may than lead to successful structure determination based on sulfur anomalous data from just one crystal collected at just
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Weiss, M S
[1]
- EMBL Hamburg Outstation c/o DESY, Hamburg (Germany)
Citation Formats
Weiss, M S.
The use of longer X-ray wavelengths in protein crystallography[Full text article has been submitted to the ''Journal of Alloys and Compounds'' (Elsevier)].
Poland: N. p.,
2004.
Web.
Weiss, M S.
The use of longer X-ray wavelengths in protein crystallography[Full text article has been submitted to the ''Journal of Alloys and Compounds'' (Elsevier)].
Poland.
Weiss, M S.
2004.
"The use of longer X-ray wavelengths in protein crystallography[Full text article has been submitted to the ''Journal of Alloys and Compounds'' (Elsevier)]."
Poland.
@misc{etde_20616787,
title = {The use of longer X-ray wavelengths in protein crystallography[Full text article has been submitted to the ''Journal of Alloys and Compounds'' (Elsevier)]}
author = {Weiss, M S}
abstractNote = {In the past, the use of longer X-ray wavelengths ({lambda} = 1.5 A -3 A) has become quite popular in macromolecular crystallography. The main reason for this is that the anomalous signal which can be obtained from the light atoms (P, S, C, Ca, etc.), from the medium heavy atoms (I, Xe, etc) and from the very heavy atoms (U etc.) can be significantly enhanced. Therefore, data collection at longer X-ray wavelength's may lead to significant advantage for experimental phase determination. It has been shown previously, that the experimental difficulties associated with the use of these longer wavelength's, namely absorption, can be dealt with sufficiently by proper scaling and that as a consequence these longer wavelength's could be utilized on a routine basis. Nevertheless, in the case of the light atoms, the obtainable anomalous signal is still very small, therefore it is absolutely essential that the data are collected with very high accuracy. As was shown previously, if the data are collected at high redundancy, very small errors in the anomalous differences can be obtained. The combination of the three effects may than lead to successful structure determination based on sulfur anomalous data from just one crystal collected at just one wavelength in an approach which follows Wang's suggestion from 1985. Using a number of case studies, the experimental requirements for longer wavelength's diffraction data collection will be discussed, as well as some suitable phasing protocols and early indicators for the success of the approach. (author)}
place = {Poland}
year = {2004}
month = {Jul}
}
title = {The use of longer X-ray wavelengths in protein crystallography[Full text article has been submitted to the ''Journal of Alloys and Compounds'' (Elsevier)]}
author = {Weiss, M S}
abstractNote = {In the past, the use of longer X-ray wavelengths ({lambda} = 1.5 A -3 A) has become quite popular in macromolecular crystallography. The main reason for this is that the anomalous signal which can be obtained from the light atoms (P, S, C, Ca, etc.), from the medium heavy atoms (I, Xe, etc) and from the very heavy atoms (U etc.) can be significantly enhanced. Therefore, data collection at longer X-ray wavelength's may lead to significant advantage for experimental phase determination. It has been shown previously, that the experimental difficulties associated with the use of these longer wavelength's, namely absorption, can be dealt with sufficiently by proper scaling and that as a consequence these longer wavelength's could be utilized on a routine basis. Nevertheless, in the case of the light atoms, the obtainable anomalous signal is still very small, therefore it is absolutely essential that the data are collected with very high accuracy. As was shown previously, if the data are collected at high redundancy, very small errors in the anomalous differences can be obtained. The combination of the three effects may than lead to successful structure determination based on sulfur anomalous data from just one crystal collected at just one wavelength in an approach which follows Wang's suggestion from 1985. Using a number of case studies, the experimental requirements for longer wavelength's diffraction data collection will be discussed, as well as some suitable phasing protocols and early indicators for the success of the approach. (author)}
place = {Poland}
year = {2004}
month = {Jul}
}