Abstract
Recent studies have shown that bone marrow (BM) cells, including the BM side population (BM-SP) cells that enrich hematopoietic stem cells (HSCs), are incorporated into skeletal muscle during regeneration, but it is not clear how and what kinds of BM cells contribute to muscle fiber regeneration. We found that a large number of SP cells migrated from BM to muscles following injury in BM-transplanted mice. These BM-derived SP cells in regenerating muscles expressed different surface markers from those of HSCs and could not reconstitute the mouse blood system. BM-derived SP/Mac-1{sup low} cells increased in number in regenerating muscles following injury. Importantly, our co-culture studies with activated satellite cells revealed that this fraction carried significant potential for myogenic differentiation. By contrast, mature inflammatory (Mac-1{sup high}) cells showed negligible myogenic activities. Further, these BM-derived SP/Mac-1{sup low} cells gave rise to mononucleate myocytes, indicating that their myogenesis was not caused by stochastic fusion with host myogenic cells, although they required cell-to-cell contact with myogenic cells for muscle differentiation. Taken together, our data suggest that neither HSCs nor mature inflammatory cells, but Mac-1{sup low} early myeloid cells in the BM-derived SP fraction, play an important role in regenerating skeletal muscles.
Ojima, Koichi;
[1]
Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)];
Uezumi, Akiyoshi;
[1]
Miyoshi, Hiroyuki;
[2]
Masuda, Satoru;
[1]
Morita, Yohei;
[3]
Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)];
Fukase, Akiko;
[1]
Hattori, Akihito;
[4]
Nakauchi, Hiromitsu;
[3]
Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)]
[5]
- Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502 (Japan)
- BioResource Center, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074 (Japan)
- Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 (Japan)
- Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)
- and others
Citation Formats
Ojima, Koichi, Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)], Uezumi, Akiyoshi, Miyoshi, Hiroyuki, Masuda, Satoru, Morita, Yohei, Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)], Fukase, Akiko, Hattori, Akihito, Nakauchi, Hiromitsu, and Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)].
Mac-1{sup low} early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration.
United States: N. p.,
2004.
Web.
doi:10.1016/j.bbrc.2004.07.069.
Ojima, Koichi, Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)], Uezumi, Akiyoshi, Miyoshi, Hiroyuki, Masuda, Satoru, Morita, Yohei, Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)], Fukase, Akiko, Hattori, Akihito, Nakauchi, Hiromitsu, & Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)].
Mac-1{sup low} early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration.
United States.
https://doi.org/10.1016/j.bbrc.2004.07.069
Ojima, Koichi, Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)], Uezumi, Akiyoshi, Miyoshi, Hiroyuki, Masuda, Satoru, Morita, Yohei, Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)], Fukase, Akiko, Hattori, Akihito, Nakauchi, Hiromitsu, and Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)].
2004.
"Mac-1{sup low} early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration."
United States.
https://doi.org/10.1016/j.bbrc.2004.07.069.
@misc{etde_20606674,
title = {Mac-1{sup low} early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration}
author = {Ojima, Koichi, Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)], Uezumi, Akiyoshi, Miyoshi, Hiroyuki, Masuda, Satoru, Morita, Yohei, Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)], Fukase, Akiko, Hattori, Akihito, Nakauchi, Hiromitsu, and Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)]}
abstractNote = {Recent studies have shown that bone marrow (BM) cells, including the BM side population (BM-SP) cells that enrich hematopoietic stem cells (HSCs), are incorporated into skeletal muscle during regeneration, but it is not clear how and what kinds of BM cells contribute to muscle fiber regeneration. We found that a large number of SP cells migrated from BM to muscles following injury in BM-transplanted mice. These BM-derived SP cells in regenerating muscles expressed different surface markers from those of HSCs and could not reconstitute the mouse blood system. BM-derived SP/Mac-1{sup low} cells increased in number in regenerating muscles following injury. Importantly, our co-culture studies with activated satellite cells revealed that this fraction carried significant potential for myogenic differentiation. By contrast, mature inflammatory (Mac-1{sup high}) cells showed negligible myogenic activities. Further, these BM-derived SP/Mac-1{sup low} cells gave rise to mononucleate myocytes, indicating that their myogenesis was not caused by stochastic fusion with host myogenic cells, although they required cell-to-cell contact with myogenic cells for muscle differentiation. Taken together, our data suggest that neither HSCs nor mature inflammatory cells, but Mac-1{sup low} early myeloid cells in the BM-derived SP fraction, play an important role in regenerating skeletal muscles.}
doi = {10.1016/j.bbrc.2004.07.069}
journal = []
issue = {4}
volume = {321}
journal type = {AC}
place = {United States}
year = {2004}
month = {Sep}
}
title = {Mac-1{sup low} early myeloid cells in the bone marrow-derived SP fraction migrate into injured skeletal muscle and participate in muscle regeneration}
author = {Ojima, Koichi, Department of Animal Science, Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589 (Japan)], Uezumi, Akiyoshi, Miyoshi, Hiroyuki, Masuda, Satoru, Morita, Yohei, Department of Immunology, Institute of Basic Medical Sciences, The University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)], Fukase, Akiko, Hattori, Akihito, Nakauchi, Hiromitsu, and Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575 (Japan)]}
abstractNote = {Recent studies have shown that bone marrow (BM) cells, including the BM side population (BM-SP) cells that enrich hematopoietic stem cells (HSCs), are incorporated into skeletal muscle during regeneration, but it is not clear how and what kinds of BM cells contribute to muscle fiber regeneration. We found that a large number of SP cells migrated from BM to muscles following injury in BM-transplanted mice. These BM-derived SP cells in regenerating muscles expressed different surface markers from those of HSCs and could not reconstitute the mouse blood system. BM-derived SP/Mac-1{sup low} cells increased in number in regenerating muscles following injury. Importantly, our co-culture studies with activated satellite cells revealed that this fraction carried significant potential for myogenic differentiation. By contrast, mature inflammatory (Mac-1{sup high}) cells showed negligible myogenic activities. Further, these BM-derived SP/Mac-1{sup low} cells gave rise to mononucleate myocytes, indicating that their myogenesis was not caused by stochastic fusion with host myogenic cells, although they required cell-to-cell contact with myogenic cells for muscle differentiation. Taken together, our data suggest that neither HSCs nor mature inflammatory cells, but Mac-1{sup low} early myeloid cells in the BM-derived SP fraction, play an important role in regenerating skeletal muscles.}
doi = {10.1016/j.bbrc.2004.07.069}
journal = []
issue = {4}
volume = {321}
journal type = {AC}
place = {United States}
year = {2004}
month = {Sep}
}