Abstract
Oxidative modification of low-density lipoprotein (LDL) is regarded as a crucial event in atherogenesis. Assessing the metabolic fate of oxidized LDL (oxLDL) in vivo with radiotracer techniques is hindered by the lack of suitable sensitive and specific radiolabeling methods. We evaluated an improved methodology based on the radiolabeling of native LDL (nLDL) and oxLDL with the positron emitter fluorine-18 ({sup 18}F) by conjugation with N-succinimidyl-4-[{sup 18}F]fluorobenzoate ([{sup 18}F]SFB). We investigated whether radiolabeling of LDL induces adverse structural modifications. Results suggest that radiolabeling of both nLDL and oxLDL using [{sup 18}F]SFB causes neither additional oxidative structural modifications of LDL lipids and proteins nor alteration of their biological activity and functionality, respectively. Thus, radiolabeling of LDL using [{sup 18}F]SFB could prove to be a promising approach for studying the kinetics of oxLDL in vivo.
Pietzsch, Jens;
[1]
Bergmann, Ralf;
[1]
Rode, Katrin;
[1]
Hultsch, Christina;
[1]
Pawelke, Beate;
[1]
Wuest, Frank;
[1]
Hoff, Joerg van den
[1]
- PET-Center, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Research Center Rossendorf Dresden, P.O. Box 51 01 19, D-01314 Dresden (Germany)
Citation Formats
Pietzsch, Jens, Bergmann, Ralf, Rode, Katrin, Hultsch, Christina, Pawelke, Beate, Wuest, Frank, and Hoff, Joerg van den.
Fluorine-18 radiolabeling of low-density lipoproteins: a potential approach for characterization and differentiation of metabolism of native and oxidized low-density lipoproteins in vivo.
United Kingdom: N. p.,
2004.
Web.
doi:10.1016/j.nucmedbio.2004.08.009.
Pietzsch, Jens, Bergmann, Ralf, Rode, Katrin, Hultsch, Christina, Pawelke, Beate, Wuest, Frank, & Hoff, Joerg van den.
Fluorine-18 radiolabeling of low-density lipoproteins: a potential approach for characterization and differentiation of metabolism of native and oxidized low-density lipoproteins in vivo.
United Kingdom.
https://doi.org/10.1016/j.nucmedbio.2004.08.009
Pietzsch, Jens, Bergmann, Ralf, Rode, Katrin, Hultsch, Christina, Pawelke, Beate, Wuest, Frank, and Hoff, Joerg van den.
2004.
"Fluorine-18 radiolabeling of low-density lipoproteins: a potential approach for characterization and differentiation of metabolism of native and oxidized low-density lipoproteins in vivo."
United Kingdom.
https://doi.org/10.1016/j.nucmedbio.2004.08.009.
@misc{etde_20602317,
title = {Fluorine-18 radiolabeling of low-density lipoproteins: a potential approach for characterization and differentiation of metabolism of native and oxidized low-density lipoproteins in vivo}
author = {Pietzsch, Jens, Bergmann, Ralf, Rode, Katrin, Hultsch, Christina, Pawelke, Beate, Wuest, Frank, and Hoff, Joerg van den}
abstractNote = {Oxidative modification of low-density lipoprotein (LDL) is regarded as a crucial event in atherogenesis. Assessing the metabolic fate of oxidized LDL (oxLDL) in vivo with radiotracer techniques is hindered by the lack of suitable sensitive and specific radiolabeling methods. We evaluated an improved methodology based on the radiolabeling of native LDL (nLDL) and oxLDL with the positron emitter fluorine-18 ({sup 18}F) by conjugation with N-succinimidyl-4-[{sup 18}F]fluorobenzoate ([{sup 18}F]SFB). We investigated whether radiolabeling of LDL induces adverse structural modifications. Results suggest that radiolabeling of both nLDL and oxLDL using [{sup 18}F]SFB causes neither additional oxidative structural modifications of LDL lipids and proteins nor alteration of their biological activity and functionality, respectively. Thus, radiolabeling of LDL using [{sup 18}F]SFB could prove to be a promising approach for studying the kinetics of oxLDL in vivo.}
doi = {10.1016/j.nucmedbio.2004.08.009}
journal = []
issue = {8}
volume = {31}
journal type = {AC}
place = {United Kingdom}
year = {2004}
month = {Nov}
}
title = {Fluorine-18 radiolabeling of low-density lipoproteins: a potential approach for characterization and differentiation of metabolism of native and oxidized low-density lipoproteins in vivo}
author = {Pietzsch, Jens, Bergmann, Ralf, Rode, Katrin, Hultsch, Christina, Pawelke, Beate, Wuest, Frank, and Hoff, Joerg van den}
abstractNote = {Oxidative modification of low-density lipoprotein (LDL) is regarded as a crucial event in atherogenesis. Assessing the metabolic fate of oxidized LDL (oxLDL) in vivo with radiotracer techniques is hindered by the lack of suitable sensitive and specific radiolabeling methods. We evaluated an improved methodology based on the radiolabeling of native LDL (nLDL) and oxLDL with the positron emitter fluorine-18 ({sup 18}F) by conjugation with N-succinimidyl-4-[{sup 18}F]fluorobenzoate ([{sup 18}F]SFB). We investigated whether radiolabeling of LDL induces adverse structural modifications. Results suggest that radiolabeling of both nLDL and oxLDL using [{sup 18}F]SFB causes neither additional oxidative structural modifications of LDL lipids and proteins nor alteration of their biological activity and functionality, respectively. Thus, radiolabeling of LDL using [{sup 18}F]SFB could prove to be a promising approach for studying the kinetics of oxLDL in vivo.}
doi = {10.1016/j.nucmedbio.2004.08.009}
journal = []
issue = {8}
volume = {31}
journal type = {AC}
place = {United Kingdom}
year = {2004}
month = {Nov}
}