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Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer

Abstract

Plant regeneration was achieved in grasspea (Lathyrus sativus L.) by in vitro shoot proliferation in cotyledonary nodes from axenically grown seedlings, de novo shoot organogenesis in callus cultures or adventitious shoot formation directly from explants. Factors influencing Agrobacterium-mediated genetic transformation were optimized using a binary vector with T-DNA cassette carrying the selectable marker nptII and the reporter gene gus-intron. The detection of GUS activity in glasshousegrown primary transformants substantiated a stable integration and expression of the gus-intron gene. Mutagenesis was induced using {gamma}-irradiation as well as two chemical mutagens viz. ethyl methane sulphonate (EMS) and N-methyl-N-nitro-nitrosoguanidine (NG). The implications of these investigations are discussed in the context of producing somaclonal variants, genetic transformants or mutants with a reduced level of the neurotoxin ODAP. (author)
Authors:
Barik, D P; Chand, P K; Mohapatra, U [1] 
  1. Post-Graduate Department of Botany, Utkal University, Orissa (India)
Publication Date:
Nov 01, 2004
Product Type:
Conference
Report Number:
IAEA-TECDOC-1426
Resource Relation:
Conference: Final research coordination meeting on genetic improvement of under-utilized and neglected crops in low income food deficit countries through irradiation and related techniques, Pretoria (South Africa), 19-23 May 2003; Other Information: 12 refs, 3 figs, 12 tabs; PBD: Nov 2004; Related Information: In: Genetic improvement of under-utilized and neglected crops in low income food deficit countries through irradiation and related techniques. Proceedings of a final research coordination meeting, 227 pages.
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; DNA; DRY STORAGE; IN VITRO; INHIBITION; LEGUMINOSAE; METHANE; MUTAGENESIS; PEAS; PROTEINS; RADIATION INDUCED MUTANTS; SEEDLINGS; TOXICITY; TOXINS
OSTI ID:
20601306
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: Contract FAO/IAEA 10424; ISBN 92-0-113604-8; ISSN 1011-4289; TRN: XA0401462047818
Availability:
Available from INIS in electronic form; Also available on-line: http://www-pub.iaea.org/MTCD/publications/PDF/te_1426_web.pdf; For availability on CD-ROM, please contact IAEA, Sales and Promotion Unit, E-mail: sales.publications@iaea.org; Web site: http://www-pub.iaea.org/MTCD/publications.asp
Submitting Site:
INIS
Size:
page(s) 57-71
Announcement Date:

Citation Formats

Barik, D P, Chand, P K, and Mohapatra, U. Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer. IAEA: N. p., 2004. Web.
Barik, D P, Chand, P K, & Mohapatra, U. Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer. IAEA.
Barik, D P, Chand, P K, and Mohapatra, U. 2004. "Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer." IAEA.
@misc{etde_20601306,
title = {Suppression of the neurotoxic amino acid in seed storage protein of Lathyrus sativus L. via mutation techniques and gene transfer}
author = {Barik, D P, Chand, P K, and Mohapatra, U}
abstractNote = {Plant regeneration was achieved in grasspea (Lathyrus sativus L.) by in vitro shoot proliferation in cotyledonary nodes from axenically grown seedlings, de novo shoot organogenesis in callus cultures or adventitious shoot formation directly from explants. Factors influencing Agrobacterium-mediated genetic transformation were optimized using a binary vector with T-DNA cassette carrying the selectable marker nptII and the reporter gene gus-intron. The detection of GUS activity in glasshousegrown primary transformants substantiated a stable integration and expression of the gus-intron gene. Mutagenesis was induced using {gamma}-irradiation as well as two chemical mutagens viz. ethyl methane sulphonate (EMS) and N-methyl-N-nitro-nitrosoguanidine (NG). The implications of these investigations are discussed in the context of producing somaclonal variants, genetic transformants or mutants with a reduced level of the neurotoxin ODAP. (author)}
place = {IAEA}
year = {2004}
month = {Nov}
}