Abstract
Objective: To investigate the effect of low dose radiation (LDR) with different dose of X-rays on P53 and Bcl-2 protein expression in spermatogenic cells of male Kunming mouse testis. Methods: The relationships between time-effect and dose-effect of P53 and Bcl-2 protein expression positive rate in spermatogenic cells of mouse testis after LDR with different dose of X-rays were studied with immunohistochemical technique (SABC). Results: P53 and Bcl-2 protein expressed in spermatogonia and spermatocytes in varying degrees, the positive rate of spermatogonia was obviously superior to that of spermatocytes. With the increase of irradiation dose, the expression of P53 protein showed a increasing tendency, however, the P53 protein expression of spermatozoa scarcely occurred after LDR. Bcl-2 protein was primarily expressed in spermatozoa. With the increase of irradiation dose, the positive rate of Bcl-2 protein expression showed a downregulated tendency. However, the Bcl-2 protein expression of spermatogonia and spermatocytes scarcely occurred after LDR. Conclusion: The expressions of P53 and Bcl-2 may have regular changes in mouse testis induced by LDR, which may provide a experimental evidence for the mechanism study of spermatogonic cell apoptosis induced selectively by ionizing radiation.
Guangwei, Liu;
Lihua, Dong;
Zhenqi, Wang;
Chunnan, Piao;
Hongguang, Zhao;
Shouliang, Gong;
[1]
Lianfu, Zhou
[2]
- Jilin Univ., Changchun (China). School of Public Health, MH Radiobiology Research Unit
- Institute of Birth Control of Jilin Province, Changchun (China)
Citation Formats
Guangwei, Liu, Lihua, Dong, Zhenqi, Wang, Chunnan, Piao, Hongguang, Zhao, Shouliang, Gong, and Lianfu, Zhou.
Effect of low dose radiation on P53 and Bcl-2 protein expression in spermatogenic cells of mouse testis.
China: N. p.,
2004.
Web.
Guangwei, Liu, Lihua, Dong, Zhenqi, Wang, Chunnan, Piao, Hongguang, Zhao, Shouliang, Gong, & Lianfu, Zhou.
Effect of low dose radiation on P53 and Bcl-2 protein expression in spermatogenic cells of mouse testis.
China.
Guangwei, Liu, Lihua, Dong, Zhenqi, Wang, Chunnan, Piao, Hongguang, Zhao, Shouliang, Gong, and Lianfu, Zhou.
2004.
"Effect of low dose radiation on P53 and Bcl-2 protein expression in spermatogenic cells of mouse testis."
China.
@misc{etde_20599940,
title = {Effect of low dose radiation on P53 and Bcl-2 protein expression in spermatogenic cells of mouse testis}
author = {Guangwei, Liu, Lihua, Dong, Zhenqi, Wang, Chunnan, Piao, Hongguang, Zhao, Shouliang, Gong, and Lianfu, Zhou}
abstractNote = {Objective: To investigate the effect of low dose radiation (LDR) with different dose of X-rays on P53 and Bcl-2 protein expression in spermatogenic cells of male Kunming mouse testis. Methods: The relationships between time-effect and dose-effect of P53 and Bcl-2 protein expression positive rate in spermatogenic cells of mouse testis after LDR with different dose of X-rays were studied with immunohistochemical technique (SABC). Results: P53 and Bcl-2 protein expressed in spermatogonia and spermatocytes in varying degrees, the positive rate of spermatogonia was obviously superior to that of spermatocytes. With the increase of irradiation dose, the expression of P53 protein showed a increasing tendency, however, the P53 protein expression of spermatozoa scarcely occurred after LDR. Bcl-2 protein was primarily expressed in spermatozoa. With the increase of irradiation dose, the positive rate of Bcl-2 protein expression showed a downregulated tendency. However, the Bcl-2 protein expression of spermatogonia and spermatocytes scarcely occurred after LDR. Conclusion: The expressions of P53 and Bcl-2 may have regular changes in mouse testis induced by LDR, which may provide a experimental evidence for the mechanism study of spermatogonic cell apoptosis induced selectively by ionizing radiation.}
journal = []
issue = {1}
volume = {30}
journal type = {AC}
place = {China}
year = {2004}
month = {Jan}
}
title = {Effect of low dose radiation on P53 and Bcl-2 protein expression in spermatogenic cells of mouse testis}
author = {Guangwei, Liu, Lihua, Dong, Zhenqi, Wang, Chunnan, Piao, Hongguang, Zhao, Shouliang, Gong, and Lianfu, Zhou}
abstractNote = {Objective: To investigate the effect of low dose radiation (LDR) with different dose of X-rays on P53 and Bcl-2 protein expression in spermatogenic cells of male Kunming mouse testis. Methods: The relationships between time-effect and dose-effect of P53 and Bcl-2 protein expression positive rate in spermatogenic cells of mouse testis after LDR with different dose of X-rays were studied with immunohistochemical technique (SABC). Results: P53 and Bcl-2 protein expressed in spermatogonia and spermatocytes in varying degrees, the positive rate of spermatogonia was obviously superior to that of spermatocytes. With the increase of irradiation dose, the expression of P53 protein showed a increasing tendency, however, the P53 protein expression of spermatozoa scarcely occurred after LDR. Bcl-2 protein was primarily expressed in spermatozoa. With the increase of irradiation dose, the positive rate of Bcl-2 protein expression showed a downregulated tendency. However, the Bcl-2 protein expression of spermatogonia and spermatocytes scarcely occurred after LDR. Conclusion: The expressions of P53 and Bcl-2 may have regular changes in mouse testis induced by LDR, which may provide a experimental evidence for the mechanism study of spermatogonic cell apoptosis induced selectively by ionizing radiation.}
journal = []
issue = {1}
volume = {30}
journal type = {AC}
place = {China}
year = {2004}
month = {Jan}
}