Abstract
With the aim of developing new strategies of control to limit the damages inflicted on fruit crops by Ceratitis capitata (Wied.) (Medfly), a biotechnological approach is undertaken whereby female viability would be impaired or male viability would be improved following the introduction of specific genes into the genome of C. capitata. Only males will then be mass produced and released in the infested areas after sterilisation (Louis et al. 1987). Such conditional lethal or 'advantageous' genes could be expressed in transgenic flies either female-specifically or male-specifically by using cis regulative sequences obtained from previously isolated endogenous Ceratitis genes (Saccone et al. 1996, 1998). By using molecular strategies based on a subtractive technique, we have recently isolated male-specifically expressed genes in the Medfly. Furthermore, we present the current status of the research on the Ceratitis dsx gene, showing sex-specific alternative splicing as in Drosophila, and on the tra-inaZ strategy to induce in Drosophila flies female-specific conditional lethality.
Saccone, G;
[1]
Pane, A;
Testa, G;
Santoro, M;
De Martino, G;
Di Paola, F;
Polito, L C;
[1]
Louis, C
[2]
- Dipartimento di Genetica, Biologia Generale e Molecolare, Universita 'Federico II' di Napoli, Naples (Italy)
- Dept. of Biology, University of Crete and Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete (Greece)
Citation Formats
Saccone, G, Pane, A, Testa, G, Santoro, M, De Martino, G, Di Paola, F, Polito, L C, and Louis, C.
Sex determination in Medfly: A molecular approach.
IAEA: N. p.,
2000.
Web.
Saccone, G, Pane, A, Testa, G, Santoro, M, De Martino, G, Di Paola, F, Polito, L C, & Louis, C.
Sex determination in Medfly: A molecular approach.
IAEA.
Saccone, G, Pane, A, Testa, G, Santoro, M, De Martino, G, Di Paola, F, Polito, L C, and Louis, C.
2000.
"Sex determination in Medfly: A molecular approach."
IAEA.
@misc{etde_20598667,
title = {Sex determination in Medfly: A molecular approach}
author = {Saccone, G, Pane, A, Testa, G, Santoro, M, De Martino, G, Di Paola, F, Polito, L C, and Louis, C}
abstractNote = {With the aim of developing new strategies of control to limit the damages inflicted on fruit crops by Ceratitis capitata (Wied.) (Medfly), a biotechnological approach is undertaken whereby female viability would be impaired or male viability would be improved following the introduction of specific genes into the genome of C. capitata. Only males will then be mass produced and released in the infested areas after sterilisation (Louis et al. 1987). Such conditional lethal or 'advantageous' genes could be expressed in transgenic flies either female-specifically or male-specifically by using cis regulative sequences obtained from previously isolated endogenous Ceratitis genes (Saccone et al. 1996, 1998). By using molecular strategies based on a subtractive technique, we have recently isolated male-specifically expressed genes in the Medfly. Furthermore, we present the current status of the research on the Ceratitis dsx gene, showing sex-specific alternative splicing as in Drosophila, and on the tra-inaZ strategy to induce in Drosophila flies female-specific conditional lethality.}
place = {IAEA}
year = {2000}
month = {Jul}
}
title = {Sex determination in Medfly: A molecular approach}
author = {Saccone, G, Pane, A, Testa, G, Santoro, M, De Martino, G, Di Paola, F, Polito, L C, and Louis, C}
abstractNote = {With the aim of developing new strategies of control to limit the damages inflicted on fruit crops by Ceratitis capitata (Wied.) (Medfly), a biotechnological approach is undertaken whereby female viability would be impaired or male viability would be improved following the introduction of specific genes into the genome of C. capitata. Only males will then be mass produced and released in the infested areas after sterilisation (Louis et al. 1987). Such conditional lethal or 'advantageous' genes could be expressed in transgenic flies either female-specifically or male-specifically by using cis regulative sequences obtained from previously isolated endogenous Ceratitis genes (Saccone et al. 1996, 1998). By using molecular strategies based on a subtractive technique, we have recently isolated male-specifically expressed genes in the Medfly. Furthermore, we present the current status of the research on the Ceratitis dsx gene, showing sex-specific alternative splicing as in Drosophila, and on the tra-inaZ strategy to induce in Drosophila flies female-specific conditional lethality.}
place = {IAEA}
year = {2000}
month = {Jul}
}