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A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function

Abstract

We combined the specificity of tumor-specific antibody with the chemokine function of interferon-{gamma} inducible protein 10 (IP-10) to recruit immune effector cells in the vicinity of tumor cells. A novel fusion protein of IP10-scFv was constructed by fusing mouse IP-10 to V{sub H} region of single-chain Fv fragment (scFv) against acidic isoferritin (AIF), and expressed in NS0 murine myeloma cells. The IP10-scFv fusion protein was shown to maintain the specificity of the antiAIF scFv with similar affinity constant, and bind to the human hepatocarcinoma SMMC 7721 cells secreting AIF as well as the activated mouse T lymphocytes expressing CXCR3 receptor. Furthermore, the IP10-scFv protein either in solution or bound on the surface of SMMC 7721 cells induced significant chemotaxis of mouse T cells in vitro. The results indicate that the IP10-scFv fusion protein possesses both bioactivities of the tumor-specific antibody and IP-10 chemokine, suggesting its possibility to induce an enhanced immune response against the residual tumor cells in vivo.
Publication Date:
Jul 23, 2004
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 320; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2004.05.193; PII: S0006291X0401201X; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); PBD: 23 Jul 2004
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; IMMUNOTHERAPY; INTERFERON; LYMPHOCYTES; MICE; NEOPLASMS; RECEPTORS; TUMOR CELLS
OSTI ID:
20596855
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US05R0256043239
Submitting Site:
INIS
Size:
page(s) 506-513
Announcement Date:
Jun 12, 2005

Citation Formats

Junqing, Guo, Liu, Chen, Hongwu, Ai, Jiannian, Jing, Jiyong, Zhou, Chuyu, Zhang, and Shangyou, You. A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function. United States: N. p., 2004. Web. doi:10.1016/j.bbrc.2004.05.193.
Junqing, Guo, Liu, Chen, Hongwu, Ai, Jiannian, Jing, Jiyong, Zhou, Chuyu, Zhang, & Shangyou, You. A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function. United States. doi:10.1016/j.bbrc.2004.05.193.
Junqing, Guo, Liu, Chen, Hongwu, Ai, Jiannian, Jing, Jiyong, Zhou, Chuyu, Zhang, and Shangyou, You. 2004. "A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function." United States. doi:10.1016/j.bbrc.2004.05.193. https://www.osti.gov/servlets/purl/10.1016/j.bbrc.2004.05.193.
@misc{etde_20596855,
title = {A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function}
author = {Junqing, Guo, Liu, Chen, Hongwu, Ai, Jiannian, Jing, Jiyong, Zhou, Chuyu, Zhang, and Shangyou, You}
abstractNote = {We combined the specificity of tumor-specific antibody with the chemokine function of interferon-{gamma} inducible protein 10 (IP-10) to recruit immune effector cells in the vicinity of tumor cells. A novel fusion protein of IP10-scFv was constructed by fusing mouse IP-10 to V{sub H} region of single-chain Fv fragment (scFv) against acidic isoferritin (AIF), and expressed in NS0 murine myeloma cells. The IP10-scFv fusion protein was shown to maintain the specificity of the antiAIF scFv with similar affinity constant, and bind to the human hepatocarcinoma SMMC 7721 cells secreting AIF as well as the activated mouse T lymphocytes expressing CXCR3 receptor. Furthermore, the IP10-scFv protein either in solution or bound on the surface of SMMC 7721 cells induced significant chemotaxis of mouse T cells in vitro. The results indicate that the IP10-scFv fusion protein possesses both bioactivities of the tumor-specific antibody and IP-10 chemokine, suggesting its possibility to induce an enhanced immune response against the residual tumor cells in vivo.}
doi = {10.1016/j.bbrc.2004.05.193}
journal = {Biochemical and Biophysical Research Communications}
issue = {2}
volume = {320}
journal type = {AC}
place = {United States}
year = {2004}
month = {Jul}
}