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Primary pulmonary immunity to a soluble antigen in the dog

Abstract

In order to study primary lung immunity to soluble antigen, Beagle dogs underwent trans-bronchoscopic instillation of 10 mg keyhole limpet hemocyanin (KLH) into the right cardiac lobe and saline into the left cardiac lobe. Over the next 3 wk, specific immune responses were monitored in blood and bronchoalveolar lavage (BAL) fluids obtained from immunized and control lung lobes. Primary lung immunization of dogs with KLH resulted in easily measurable specific IgG, IgM, and IgA responses in blood and bronchoalveolar lavage fluids. Important class differences were noted to exist for these responses. Levels of specific IgG and IgA in BAL remained elevated at 21 days after immunization, while BAL-specific IgM levels fell to control values by 12 days after immunization. In unimmunized lung lobes, specific antibody levels in BAL and production of specific antibody by bronchoalveolar lavage cells was far greater for IgG than for IgM or IgA. Finally, despite easily detectable specific IgA in serum and BAL fluid, production of specific IgA by peripheral blood mononuclear cells or bronchoalveolar lavage cells after primary lung immunization with KLH could not be demonstrated, suggesting that specific IgA was produced by a compartment of cells poorly sampled in blood and BAL. (author)
Publication Date:
Dec 01, 1988
Product Type:
Technical Report
Report Number:
LMF-121; INIS-XA-N-170
Resource Relation:
Other Information: 5 refs, 4 figs; PBD: Dec 1988; Related Information: In: Inhalation Toxicology Research Institute annual report 1987-1988, by Mauderly, J.L.; Mewhinney, J.A.; Bechtold, W.E.; Sun, J.D.; Coons, T.A. (eds.), 659 pages.
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ANTIGENS; BEAGLES; BLOOD; HEMOCYANIN; IMMUNITY; LAVAGE; LUNGS; MONITORS
Sponsoring Organizations:
Office of Health and Environmental Research, U.S. Department of Energy (United States)
OSTI ID:
20547838
Research Organizations:
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: Contract DE-AC04-76EV01013; TRN: XA04N1404003668
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 458-463
Announcement Date:

Citation Formats

Weissman, D N, Bice, D E, Muggenburg, B A, and Schuyler, M R. Primary pulmonary immunity to a soluble antigen in the dog. IAEA: N. p., 1988. Web.
Weissman, D N, Bice, D E, Muggenburg, B A, & Schuyler, M R. Primary pulmonary immunity to a soluble antigen in the dog. IAEA.
Weissman, D N, Bice, D E, Muggenburg, B A, and Schuyler, M R. 1988. "Primary pulmonary immunity to a soluble antigen in the dog." IAEA.
@misc{etde_20547838,
title = {Primary pulmonary immunity to a soluble antigen in the dog}
author = {Weissman, D N, Bice, D E, Muggenburg, B A, and Schuyler, M R}
abstractNote = {In order to study primary lung immunity to soluble antigen, Beagle dogs underwent trans-bronchoscopic instillation of 10 mg keyhole limpet hemocyanin (KLH) into the right cardiac lobe and saline into the left cardiac lobe. Over the next 3 wk, specific immune responses were monitored in blood and bronchoalveolar lavage (BAL) fluids obtained from immunized and control lung lobes. Primary lung immunization of dogs with KLH resulted in easily measurable specific IgG, IgM, and IgA responses in blood and bronchoalveolar lavage fluids. Important class differences were noted to exist for these responses. Levels of specific IgG and IgA in BAL remained elevated at 21 days after immunization, while BAL-specific IgM levels fell to control values by 12 days after immunization. In unimmunized lung lobes, specific antibody levels in BAL and production of specific antibody by bronchoalveolar lavage cells was far greater for IgG than for IgM or IgA. Finally, despite easily detectable specific IgA in serum and BAL fluid, production of specific IgA by peripheral blood mononuclear cells or bronchoalveolar lavage cells after primary lung immunization with KLH could not be demonstrated, suggesting that specific IgA was produced by a compartment of cells poorly sampled in blood and BAL. (author)}
place = {IAEA}
year = {1988}
month = {Dec}
}