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Expression of Kirsten murine sarcoma virus sequences in Beagle dog tissues

Abstract

Labeled cDNA synthesized from RNA extracted from {sup 238}PuO{sub 2}-, {sup 239}PuO{sub 2}-, and {sup 90}Sr-induced lung tumors in Beagle dogs, from nontumor tissue from {sup 239}PuO{sub 2}-exposed dogs, and from unexposed dog lung and liver tissue produces strong hybridization signals with a plasmid (pKSma) that contains Kirsten murine sarcoma virus (KMSV) sequences. At least 90 percent of the KMSV sequences are expressed in these dog tissues, including sequences corresponding to p21 K-ras, qp70 envelope glycoprotein, and at least one other proviral sequence. The expression of Kirsten ras and other sarcoma virus sequences may have important implications for the interpretation of carcinogenesis studies in these dogs. (author)
Publication Date:
Dec 01, 1988
Product Type:
Technical Report
Report Number:
LMF-121; INIS-XA-N-170
Resource Relation:
Other Information: 8 refs, 4 figs, 1 tab; PBD: Dec 1988; Related Information: In: Inhalation Toxicology Research Institute annual report 1987-1988, by Mauderly, J.L.; Mewhinney, J.A.; Bechtold, W.E.; Sun, J.D.; Coons, T.A. (eds.), 659 pages.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BEAGLES; CARCINOGENESIS; GLYCOPROTEINS; HYBRIDIZATION; LIVER; LUNGS; MONOCLINIC LATTICES; PHOSPHORUS 21; PLUTONIUM 238; PLUTONIUM 239; RNA; SARCOMAS; STRONTIUM 90; VIRUSES
Sponsoring Organizations:
Office of Health and Environmental Research, U.S. Department of Energy (United States)
OSTI ID:
20547814
Research Organizations:
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: Contract DE-AC04-76EV01013; TRN: XA04N1380003644
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 338-344
Announcement Date:

Citation Formats

Kerkof, P R, and Kelly, G. Expression of Kirsten murine sarcoma virus sequences in Beagle dog tissues. IAEA: N. p., 1988. Web.
Kerkof, P R, & Kelly, G. Expression of Kirsten murine sarcoma virus sequences in Beagle dog tissues. IAEA.
Kerkof, P R, and Kelly, G. 1988. "Expression of Kirsten murine sarcoma virus sequences in Beagle dog tissues." IAEA.
@misc{etde_20547814,
title = {Expression of Kirsten murine sarcoma virus sequences in Beagle dog tissues}
author = {Kerkof, P R, and Kelly, G}
abstractNote = {Labeled cDNA synthesized from RNA extracted from {sup 238}PuO{sub 2}-, {sup 239}PuO{sub 2}-, and {sup 90}Sr-induced lung tumors in Beagle dogs, from nontumor tissue from {sup 239}PuO{sub 2}-exposed dogs, and from unexposed dog lung and liver tissue produces strong hybridization signals with a plasmid (pKSma) that contains Kirsten murine sarcoma virus (KMSV) sequences. At least 90 percent of the KMSV sequences are expressed in these dog tissues, including sequences corresponding to p21 K-ras, qp70 envelope glycoprotein, and at least one other proviral sequence. The expression of Kirsten ras and other sarcoma virus sequences may have important implications for the interpretation of carcinogenesis studies in these dogs. (author)}
place = {IAEA}
year = {1988}
month = {Dec}
}