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Regional differences in DNA replication in nasal epithelium following acute ozone or cigarette smoke exposure

Abstract

The epithelium of the anterior nasal cavity is composed of four cell types, squamous, respiratory, cuboidal, and olfactory cells. We monitored proliferation In these tissues by bromodeoxy-uridine (BrdUrd) incorporation; the labeled cells were identified by using a monoclonal antibody that recognizes BrdUrd. The respiratory, cuboidal and olfactory epithelia had low cell turnover (1-labeled ceIl/mm basal lamina). Squamous epithelium contained 40-labeled cells per mm basal lamina. Following exposure to diluted mainstream cigarette smoke, a transient, but marked increase in DNA replication was seen in the cuboidal epithelium. In contrast, ozone exposure was associated with DNA replication in the olfactory and respiratory epithelium, as well as in the cuboidal epithelium. These studies show that the sensitivity of nasal epithelium to irritants can be assayed by measuring DNA replication. (author)
Publication Date:
Dec 01, 1988
Product Type:
Technical Report
Report Number:
LMF-121; INIS-XA-N-170
Resource Relation:
Other Information: 3 refs, 2 figs; PBD: Dec 1988; Related Information: In: Inhalation Toxicology Research Institute annual report 1987-1988, by Mauderly, J.L.; Mewhinney, J.A.; Bechtold, W.E.; Sun, J.D.; Coons, T.A. (eds.), 659 pages.
Subject:
60 APPLIED LIFE SCIENCES; CAVITIES; DNA REPLICATION; EPITHELIUM; MONOCLONAL ANTIBODIES; OZONE; SENSITIVITY; TOBACCO SMOKES; URIDINE
Sponsoring Organizations:
Office of Health and Environmental Research, U.S. Department of Energy (United States)
OSTI ID:
20547802
Research Organizations:
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: Contract DE-AC04-76EV01013; TRN: XA04N1368003632
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 279-283
Announcement Date:

Citation Formats

Johnson, N F, Hotchkiss, J A, Harkema, J R, Henderson, R F, Mauderly, J L, and Cuddihy, R G. Regional differences in DNA replication in nasal epithelium following acute ozone or cigarette smoke exposure. IAEA: N. p., 1988. Web.
Johnson, N F, Hotchkiss, J A, Harkema, J R, Henderson, R F, Mauderly, J L, & Cuddihy, R G. Regional differences in DNA replication in nasal epithelium following acute ozone or cigarette smoke exposure. IAEA.
Johnson, N F, Hotchkiss, J A, Harkema, J R, Henderson, R F, Mauderly, J L, and Cuddihy, R G. 1988. "Regional differences in DNA replication in nasal epithelium following acute ozone or cigarette smoke exposure." IAEA.
@misc{etde_20547802,
title = {Regional differences in DNA replication in nasal epithelium following acute ozone or cigarette smoke exposure}
author = {Johnson, N F, Hotchkiss, J A, Harkema, J R, Henderson, R F, Mauderly, J L, and Cuddihy, R G}
abstractNote = {The epithelium of the anterior nasal cavity is composed of four cell types, squamous, respiratory, cuboidal, and olfactory cells. We monitored proliferation In these tissues by bromodeoxy-uridine (BrdUrd) incorporation; the labeled cells were identified by using a monoclonal antibody that recognizes BrdUrd. The respiratory, cuboidal and olfactory epithelia had low cell turnover (1-labeled ceIl/mm basal lamina). Squamous epithelium contained 40-labeled cells per mm basal lamina. Following exposure to diluted mainstream cigarette smoke, a transient, but marked increase in DNA replication was seen in the cuboidal epithelium. In contrast, ozone exposure was associated with DNA replication in the olfactory and respiratory epithelium, as well as in the cuboidal epithelium. These studies show that the sensitivity of nasal epithelium to irritants can be assayed by measuring DNA replication. (author)}
place = {IAEA}
year = {1988}
month = {Dec}
}