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Production and testing of {sup 244}Cm-labeled fluorescent polystyrene latex microspheres

Abstract

To provide a useful tool for studying the mechanisms of retention and translocation of respirable-sized, alpha-emitting particles, we have developed a method of incorporating {sup 244}Cm into fluorescent polystyrene latex microspheres. The resultant particles contain alpha radioactivity comparable to {mu}m-size (AMAD) {sup 239}Pu0{sub 2} particles, but are easily visible by fluorescent light microscopy. Preliminary testing of the particles with dog macrophages in vitro has shown that the initial uptake of these Cm-labeled particles is more rapid than uptake of unlabeled particles of similar size. We are continuing to develop procedures for achieving better particle yields, smaller dispersity of particle size distributions and improved retention of the Cm label by the particles. (author)
Publication Date:
Dec 01, 1988
Product Type:
Technical Report
Report Number:
LMF-121; INIS-XA-N-170
Resource Relation:
Other Information: 3 refs, 3 figs; PBD: Dec 1988; Related Information: In: Inhalation Toxicology Research Institute annual report 1987-1988, by Mauderly, J.L.; Mewhinney, J.A.; Bechtold, W.E.; Sun, J.D.; Coons, T.A. (eds.), 659 pages.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CURIUM 244; DOGS; IN VITRO; LATEX; MACROPHAGES; MICROSCOPY; MICROSPHERES; PARTICLE SIZE; PARTICLES; POLYSTYRENE; RADIOACTIVITY; RETENTION; TRANSLOCATION; UPTAKE
Sponsoring Organizations:
Office of Health and Environmental Research, U.S. Department of Energy (United States)
OSTI ID:
20547778
Research Organizations:
Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: Contract DE-AC04-76EV01013; TRN: XA04N1344003608
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 126-130
Announcement Date:
Feb 01, 2005

Citation Formats

Guilmette, R A, Mueller, H L, and Brodbeck, R D. Production and testing of {sup 244}Cm-labeled fluorescent polystyrene latex microspheres. IAEA: N. p., 1988. Web.
Guilmette, R A, Mueller, H L, & Brodbeck, R D. Production and testing of {sup 244}Cm-labeled fluorescent polystyrene latex microspheres. IAEA.
Guilmette, R A, Mueller, H L, and Brodbeck, R D. 1988. "Production and testing of {sup 244}Cm-labeled fluorescent polystyrene latex microspheres." IAEA.
@misc{etde_20547778,
title = {Production and testing of {sup 244}Cm-labeled fluorescent polystyrene latex microspheres}
author = {Guilmette, R A, Mueller, H L, and Brodbeck, R D}
abstractNote = {To provide a useful tool for studying the mechanisms of retention and translocation of respirable-sized, alpha-emitting particles, we have developed a method of incorporating {sup 244}Cm into fluorescent polystyrene latex microspheres. The resultant particles contain alpha radioactivity comparable to {mu}m-size (AMAD) {sup 239}Pu0{sub 2} particles, but are easily visible by fluorescent light microscopy. Preliminary testing of the particles with dog macrophages in vitro has shown that the initial uptake of these Cm-labeled particles is more rapid than uptake of unlabeled particles of similar size. We are continuing to develop procedures for achieving better particle yields, smaller dispersity of particle size distributions and improved retention of the Cm label by the particles. (author)}
place = {IAEA}
year = {1988}
month = {Dec}
}