Abstract
Nitric oxide (NO) has been reported to be a radiosensitizer of mammalian cells under hypoxic conditions. In a previous study, we demonstrated an enhancement in radiation response induced by NO in mouse tumor cells under aerobic conditions, with an increasing effect as a function of malignancy. The aim of the present study was to evaluate the effect of NO in tumor cells and in experimental tumors irradiated with {gamma} rays and proton beams. Irradiations were performed with a {sup 137}Cs {gamma} source and with proton beams generated by the TANDAR accelerator. Tumor cells were treated with the NO donor DETA-NO and the sensitizer enhancement ratio (SER) was calculated using the {alpha} parameter of the survival curve fitted to the linear-quadratic model. Tumor cells irradiated with protons were radio sensitized by DETA-NO only in the more malignant cells irradiated with low LET protons (2.69{+-}0.08 keV/{mu}m). For higher LET protons there were no radiosensitizing effect. For human tumor cells pre-treated with DETA-NO and irradiated with {gamma} rays, a significantly greater effect was demonstrated in the malignant cells (MCF-7) as compared with the near normal cells (HBL-100). Moreover, a significant decrease in tumor growth was demonstrated in mice pre-treated with the NO donor
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Policastro, Lucia L;
Duran, Hebe;
Molinari, Beatriz L;
[1]
Schuff, Juan A;
Kreiner, Andres J;
Burlon, Alejandro A;
Debray, Mario E;
Kesque, Jose M;
Ozafran, Mabel J;
Vazquez, Monica E;
[2]
Davidson, Jorge;
Davidson, Miguel;
[3]
Somacal, Hector R;
Valda, Alejandro A
[4]
- Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Radiobiologia
- Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Fisica
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina)
- Universidad Nacional de General San Martin , Villa Ballester (Argentina). Escuela de Ciencia y Tecnologia
Citation Formats
Policastro, Lucia L, Duran, Hebe, Molinari, Beatriz L, Schuff, Juan A, Kreiner, Andres J, Burlon, Alejandro A, Debray, Mario E, Kesque, Jose M, Ozafran, Mabel J, Vazquez, Monica E, Davidson, Jorge, Davidson, Miguel, Somacal, Hector R, and Valda, Alejandro A.
Radiosensitizing effect of nitric oxide in tumor cells and experimental tumors irradiated with gamma rays and proton beams; Efecto radiosensibilizador del oxido nitrico en celulas tumorales y en tumores experimentales irradiados con radiacion gamma y con haces de protones.
Argentina: N. p.,
2003.
Web.
Policastro, Lucia L, Duran, Hebe, Molinari, Beatriz L, Schuff, Juan A, Kreiner, Andres J, Burlon, Alejandro A, Debray, Mario E, Kesque, Jose M, Ozafran, Mabel J, Vazquez, Monica E, Davidson, Jorge, Davidson, Miguel, Somacal, Hector R, & Valda, Alejandro A.
Radiosensitizing effect of nitric oxide in tumor cells and experimental tumors irradiated with gamma rays and proton beams; Efecto radiosensibilizador del oxido nitrico en celulas tumorales y en tumores experimentales irradiados con radiacion gamma y con haces de protones.
Argentina.
Policastro, Lucia L, Duran, Hebe, Molinari, Beatriz L, Schuff, Juan A, Kreiner, Andres J, Burlon, Alejandro A, Debray, Mario E, Kesque, Jose M, Ozafran, Mabel J, Vazquez, Monica E, Davidson, Jorge, Davidson, Miguel, Somacal, Hector R, and Valda, Alejandro A.
2003.
"Radiosensitizing effect of nitric oxide in tumor cells and experimental tumors irradiated with gamma rays and proton beams; Efecto radiosensibilizador del oxido nitrico en celulas tumorales y en tumores experimentales irradiados con radiacion gamma y con haces de protones."
Argentina.
@misc{etde_20498881,
title = {Radiosensitizing effect of nitric oxide in tumor cells and experimental tumors irradiated with gamma rays and proton beams; Efecto radiosensibilizador del oxido nitrico en celulas tumorales y en tumores experimentales irradiados con radiacion gamma y con haces de protones}
author = {Policastro, Lucia L, Duran, Hebe, Molinari, Beatriz L, Schuff, Juan A, Kreiner, Andres J, Burlon, Alejandro A, Debray, Mario E, Kesque, Jose M, Ozafran, Mabel J, Vazquez, Monica E, Davidson, Jorge, Davidson, Miguel, Somacal, Hector R, and Valda, Alejandro A}
abstractNote = {Nitric oxide (NO) has been reported to be a radiosensitizer of mammalian cells under hypoxic conditions. In a previous study, we demonstrated an enhancement in radiation response induced by NO in mouse tumor cells under aerobic conditions, with an increasing effect as a function of malignancy. The aim of the present study was to evaluate the effect of NO in tumor cells and in experimental tumors irradiated with {gamma} rays and proton beams. Irradiations were performed with a {sup 137}Cs {gamma} source and with proton beams generated by the TANDAR accelerator. Tumor cells were treated with the NO donor DETA-NO and the sensitizer enhancement ratio (SER) was calculated using the {alpha} parameter of the survival curve fitted to the linear-quadratic model. Tumor cells irradiated with protons were radio sensitized by DETA-NO only in the more malignant cells irradiated with low LET protons (2.69{+-}0.08 keV/{mu}m). For higher LET protons there were no radiosensitizing effect. For human tumor cells pre-treated with DETA-NO and irradiated with {gamma} rays, a significantly greater effect was demonstrated in the malignant cells (MCF-7) as compared with the near normal cells (HBL-100). Moreover, a significant decrease in tumor growth was demonstrated in mice pre-treated with the NO donor spermine and irradiated with {gamma} rays and low LET protons as compared with mice irradiated without pre-treatment with the NO donor. In conclusion, we demonstrated a differential effect of NO as a radiosensitizer of malignant cells, both with {gamma} rays and low LET protons. This selectivity, coupled to the in vivo inhibition of tumor growth, is of great interest for the potential use of NO releasing agents in radiotherapy. (author)}
place = {Argentina}
year = {2003}
month = {Jul}
}
title = {Radiosensitizing effect of nitric oxide in tumor cells and experimental tumors irradiated with gamma rays and proton beams; Efecto radiosensibilizador del oxido nitrico en celulas tumorales y en tumores experimentales irradiados con radiacion gamma y con haces de protones}
author = {Policastro, Lucia L, Duran, Hebe, Molinari, Beatriz L, Schuff, Juan A, Kreiner, Andres J, Burlon, Alejandro A, Debray, Mario E, Kesque, Jose M, Ozafran, Mabel J, Vazquez, Monica E, Davidson, Jorge, Davidson, Miguel, Somacal, Hector R, and Valda, Alejandro A}
abstractNote = {Nitric oxide (NO) has been reported to be a radiosensitizer of mammalian cells under hypoxic conditions. In a previous study, we demonstrated an enhancement in radiation response induced by NO in mouse tumor cells under aerobic conditions, with an increasing effect as a function of malignancy. The aim of the present study was to evaluate the effect of NO in tumor cells and in experimental tumors irradiated with {gamma} rays and proton beams. Irradiations were performed with a {sup 137}Cs {gamma} source and with proton beams generated by the TANDAR accelerator. Tumor cells were treated with the NO donor DETA-NO and the sensitizer enhancement ratio (SER) was calculated using the {alpha} parameter of the survival curve fitted to the linear-quadratic model. Tumor cells irradiated with protons were radio sensitized by DETA-NO only in the more malignant cells irradiated with low LET protons (2.69{+-}0.08 keV/{mu}m). For higher LET protons there were no radiosensitizing effect. For human tumor cells pre-treated with DETA-NO and irradiated with {gamma} rays, a significantly greater effect was demonstrated in the malignant cells (MCF-7) as compared with the near normal cells (HBL-100). Moreover, a significant decrease in tumor growth was demonstrated in mice pre-treated with the NO donor spermine and irradiated with {gamma} rays and low LET protons as compared with mice irradiated without pre-treatment with the NO donor. In conclusion, we demonstrated a differential effect of NO as a radiosensitizer of malignant cells, both with {gamma} rays and low LET protons. This selectivity, coupled to the in vivo inhibition of tumor growth, is of great interest for the potential use of NO releasing agents in radiotherapy. (author)}
place = {Argentina}
year = {2003}
month = {Jul}
}