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High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection

Abstract

During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.
Publication Date:
Jul 20, 2003
Product Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 312; Journal Issue: 1; Other Information: PII: S0042682203002071; Copyright (c) 2003 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); PBD: 20 Jul 2003
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; CELL CYCLE; DNA; FIBROBLASTS; GENES; GROWTH FACTORS; HOMEOSTASIS; MUCOUS MEMBRANES; SMALL INTESTINE; TRANSCRIPTION; TRANSCRIPTION FACTORS; VIRUSES
OSTI ID:
20493698
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0042-6822; VIRLAX; TRN: US03S1792065851
Submitting Site:
INIS
Size:
page(s) 84-94
Announcement Date:
Aug 21, 2004

Citation Formats

George, Michael D, Sankaran, Sumathi, Reay, Elizabeth, Gelli, Angie C, and Dandekar, Satya. High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection. United States: N. p., 2003. Web. doi:10.1016/S0042-6822(03)00207-1.
George, Michael D, Sankaran, Sumathi, Reay, Elizabeth, Gelli, Angie C, & Dandekar, Satya. High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection. United States. doi:10.1016/S0042-6822(03)00207-1.
George, Michael D, Sankaran, Sumathi, Reay, Elizabeth, Gelli, Angie C, and Dandekar, Satya. 2003. "High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection." United States. doi:10.1016/S0042-6822(03)00207-1. https://www.osti.gov/servlets/purl/10.1016/S0042-6822(03)00207-1.
@misc{etde_20493698,
title = {High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection}
author = {George, Michael D, Sankaran, Sumathi, Reay, Elizabeth, Gelli, Angie C, and Dandekar, Satya}
abstractNote = {During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.}
doi = {10.1016/S0042-6822(03)00207-1}
journal = {Virology}
issue = {1}
volume = {312}
journal type = {AC}
place = {United States}
year = {2003}
month = {Jul}
}