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Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America

Abstract

Disease is among the suspected causes of amphibian population declines, and an iridovirus and a chytrid fungus are the primary pathogens associated with amphibian mortalities. Ambystoma tigrinum virus (ATV) and a closely related strain, Regina ranavirus (RRV), are implicated in salamander die-offs in Arizona and Canada, respectively. We report the complete sequence of the ATV genome and partial sequence of the RRV genome. Sequence analysis of the ATV/RRV genomes showed marked similarity to other ranaviruses, including tiger frog virus (TFV) and frog virus 3 (FV3), the type virus of the genus Ranavirus (family Iridoviridae), as well as more distant relationships to lymphocystis disease virus, Chilo iridescent virus, and infectious spleen and kidney necrosis virus. Putative open reading frames (ORFs) in the ATV sequence identified 24 genes that appear to control virus replication and block antiviral responses. In addition, >50 other putative genes, homologous to ORFs in other iridoviral genomes but of unknown function, were also identified. Sequence comparison performed by dot plot analysis between ATV and itself revealed a conserved 14-bp palindromic repeat within most intragenic regions. Dot plot analysis of ATV vs RRV sequences identified several polymorphisms between the two isolates. Finally, a comparison of ATV and TFV genomic  More>>
Publication Date:
Nov 10, 2003
Product Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 316; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2003.08.001; PII: S0042682203005981; Copyright (c) 2003 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); PBD: 10 Nov 2003
Subject:
60 APPLIED LIFE SCIENCES; AMPHIBIANS; FUNGI; LYMPHOCYTES; NECROSIS; STRUCTURAL CHEMICAL ANALYSIS; VIRAL DISEASES; VIRUSES
OSTI ID:
20474927
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0042-6822; VIRLAX; TRN: US04R1994050214
Submitting Site:
INIS
Size:
page(s) 90-103
Announcement Date:

Citation Formats

Jancovich, James K, Jinghe, Mao, Chinchar, V Gregory, Wyatt, Christopher, Case, Steven T, Kumar, Sudhir, Valente, Graziela, Subramanian, Sankar, Davidson, Elizabeth W, Collins, James P, and Jacobs, Bertram L. Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America. United States: N. p., 2003. Web. doi:10.1016/j.virol.2003.08.001.
Jancovich, James K, Jinghe, Mao, Chinchar, V Gregory, Wyatt, Christopher, Case, Steven T, Kumar, Sudhir, Valente, Graziela, Subramanian, Sankar, Davidson, Elizabeth W, Collins, James P, & Jacobs, Bertram L. Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America. United States. doi:10.1016/j.virol.2003.08.001.
Jancovich, James K, Jinghe, Mao, Chinchar, V Gregory, Wyatt, Christopher, Case, Steven T, Kumar, Sudhir, Valente, Graziela, Subramanian, Sankar, Davidson, Elizabeth W, Collins, James P, and Jacobs, Bertram L. 2003. "Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America." United States. doi:10.1016/j.virol.2003.08.001. https://www.osti.gov/servlets/purl/10.1016/j.virol.2003.08.001.
@misc{etde_20474927,
title = {Genomic sequence of a ranavirus (family Iridoviridae) associated with salamander mortalities in North America}
author = {Jancovich, James K, Jinghe, Mao, Chinchar, V Gregory, Wyatt, Christopher, Case, Steven T, Kumar, Sudhir, Valente, Graziela, Subramanian, Sankar, Davidson, Elizabeth W, Collins, James P, and Jacobs, Bertram L}
abstractNote = {Disease is among the suspected causes of amphibian population declines, and an iridovirus and a chytrid fungus are the primary pathogens associated with amphibian mortalities. Ambystoma tigrinum virus (ATV) and a closely related strain, Regina ranavirus (RRV), are implicated in salamander die-offs in Arizona and Canada, respectively. We report the complete sequence of the ATV genome and partial sequence of the RRV genome. Sequence analysis of the ATV/RRV genomes showed marked similarity to other ranaviruses, including tiger frog virus (TFV) and frog virus 3 (FV3), the type virus of the genus Ranavirus (family Iridoviridae), as well as more distant relationships to lymphocystis disease virus, Chilo iridescent virus, and infectious spleen and kidney necrosis virus. Putative open reading frames (ORFs) in the ATV sequence identified 24 genes that appear to control virus replication and block antiviral responses. In addition, >50 other putative genes, homologous to ORFs in other iridoviral genomes but of unknown function, were also identified. Sequence comparison performed by dot plot analysis between ATV and itself revealed a conserved 14-bp palindromic repeat within most intragenic regions. Dot plot analysis of ATV vs RRV sequences identified several polymorphisms between the two isolates. Finally, a comparison of ATV and TFV genomic sequences identified genomic rearrangements consistent with the high recombination frequency of iridoviruses. Given the adverse effects that ranavirus infections have on amphibian and fish populations, ATV/RRV sequence information will allow the design of better diagnostic probes for identifying ranavirus infections and extend our understanding of molecular events in ranavirus-infected cells.}
doi = {10.1016/j.virol.2003.08.001}
journal = {Virology}
issue = {1}
volume = {316}
journal type = {AC}
place = {United States}
year = {2003}
month = {Nov}
}