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Measurement and protection of the oxidative damage induced by heavy-ion irradiation in human glioblastoma cell lines

Technical Report:

Abstract

The aim of this study is to evaluate the oxidative damage of DNA caused by low or high linear energy transfer (LET) radiation by measuring the level of 8-hydroxydeoxyguanosine (8-OHdG). Relationship between the 8-OHdG level and the LET value was assessed, and the anti-oxidative effect of 3-methyl-1-phenyl-2-pyrazolin-5-one (edaravone) on DNA was evaluated as well. Solution containing salmon sperm DNA at a concentration of 1.0 mg/ml was irradiated with 10 Gy of x-ray or 290 MeV/u carbon beams with an LET range of 20-80 keV/{mu}m. The 8-OHdG level in the DNA solution was measured by high performance liquid chromatography (HPLC) with an electrochemical detector (ECD) after each irradiation. Edaravone was added to the DNA solution in final concentrations from 10 {mu}M to 1 mM and 8-OHdG level was measured by the same method after irradiation. The level of 8-OHdG increased dose-dependently after x-rays irradiation, and it was significantly suppressed by the presence of edaravone. The yield of 8-OHdG decreased as LET value increased, and the protection efficiency by edaravone decreased as LET value increased. (author)
Authors:
Moritake, Takashi; Tsuboi, Koji; Nose, Tadao; [1]  Anzai, Kazunori; Ikota, Nobuo; Ando, Koichi; Ozawa, Toshihiko [2] 
  1. Tsukuba Univ., Ibaraki (Japan). Inst. of Clinical Medicine
  2. National Inst. of Radiological Sciences, Chiba (Japan)
Publication Date:
Apr 01, 2003
Product Type:
Technical Report
Report Number:
NIRS-M-166; HIMAC-069
Resource Relation:
Other Information: PBD: Apr 2003; Related Information: In: 2002 annual report of the research project with heavy ions at NIRS-HIMAC, 335 pages.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; CARBON IONS; DNA; DOSE-RESPONSE RELATIONSHIPS; GUANOSINE; HIMAC ACCELERATOR; HYDROXYL RADICALS; LET; OXIDATION; RADIATION DOSES; SALMON; SPERMATOZOA; X RADIATION
OSTI ID:
20468676
Research Organizations:
National Inst. of Radiological Sciences, Chiba (Japan)
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
TRN: JP0401197044427
Availability:
Available from National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba-shi, Chiba-ken 263-8555, Japan
Submitting Site:
INIS
Size:
page(s) 125-126
Announcement Date:
Jun 21, 2004

Technical Report:

Citation Formats

Moritake, Takashi, Tsuboi, Koji, Nose, Tadao, Anzai, Kazunori, Ikota, Nobuo, Ando, Koichi, and Ozawa, Toshihiko. Measurement and protection of the oxidative damage induced by heavy-ion irradiation in human glioblastoma cell lines. Japan: N. p., 2003. Web.
Moritake, Takashi, Tsuboi, Koji, Nose, Tadao, Anzai, Kazunori, Ikota, Nobuo, Ando, Koichi, & Ozawa, Toshihiko. Measurement and protection of the oxidative damage induced by heavy-ion irradiation in human glioblastoma cell lines. Japan.
Moritake, Takashi, Tsuboi, Koji, Nose, Tadao, Anzai, Kazunori, Ikota, Nobuo, Ando, Koichi, and Ozawa, Toshihiko. 2003. "Measurement and protection of the oxidative damage induced by heavy-ion irradiation in human glioblastoma cell lines." Japan.
@misc{etde_20468676,
title = {Measurement and protection of the oxidative damage induced by heavy-ion irradiation in human glioblastoma cell lines}
author = {Moritake, Takashi, Tsuboi, Koji, Nose, Tadao, Anzai, Kazunori, Ikota, Nobuo, Ando, Koichi, and Ozawa, Toshihiko}
abstractNote = {The aim of this study is to evaluate the oxidative damage of DNA caused by low or high linear energy transfer (LET) radiation by measuring the level of 8-hydroxydeoxyguanosine (8-OHdG). Relationship between the 8-OHdG level and the LET value was assessed, and the anti-oxidative effect of 3-methyl-1-phenyl-2-pyrazolin-5-one (edaravone) on DNA was evaluated as well. Solution containing salmon sperm DNA at a concentration of 1.0 mg/ml was irradiated with 10 Gy of x-ray or 290 MeV/u carbon beams with an LET range of 20-80 keV/{mu}m. The 8-OHdG level in the DNA solution was measured by high performance liquid chromatography (HPLC) with an electrochemical detector (ECD) after each irradiation. Edaravone was added to the DNA solution in final concentrations from 10 {mu}M to 1 mM and 8-OHdG level was measured by the same method after irradiation. The level of 8-OHdG increased dose-dependently after x-rays irradiation, and it was significantly suppressed by the presence of edaravone. The yield of 8-OHdG decreased as LET value increased, and the protection efficiency by edaravone decreased as LET value increased. (author)}
place = {Japan}
year = {2003}
month = {Apr}
}