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NMR studies of human blood cells in health and disease. I. Alterations of the plasma membrane water permeability of erythrocytes

Journal Article:

Abstract

Alterations in pathological cases of the human erythrocyte membrane water permeability were investigated by using a Mn{sup 2+}-doping {sup 1}H nuclear magnetic resonance (NMR) technique. The temperature dependence of the apparent water diffusional exchange through erythrocyte membranes in chronic hepatitis, diabetes, dyslipidemia and essential hypertension was measured and compared to healthy controls. Using moderate manganese concentrations (9-18 mM) and Carr-Purcell-Meiboom-Gill pulse sequences with a large number of refocusing {pi} pulses and short interpulse delay (100 {mu}s) our values of the water exchange times ({tau}{sub e}) across erythrocyte membranes, obtained within a 10 min time period following the moment of doping, were independent of the actual manganese concentration and the Arrhenius plot for water exchange was linear over the range of 22-42 deg C. A marked increase of the water exchange times values was observed in all studied disease states. In case of chronic hepatitis, diabetes and dyslipidemia the changes observed in transmembrane water exchange time were associated with significant increase in the apparent activation energy of the diffusional water permeability thus, pointing out alterations in the function of the erythrocyte water channel. (author)
Authors:
Katona, Eva; Doaga, I O; Radulet, Diana; [1]  Caplanusi, A; [2]  Negreanu, Cezarina; [3]  Mihele, Denisa [4] 
  1. Department of Biophysics, Carol Davila University of Medicine and Pharmaceutics, 8 Blvd. Eroilor Sanitari, POB 15-205, RO-76241 Bucharest (Romania)
  2. Medical Biochemistry Department, Carol Davila University of Medicine and Pharmaceutics, 8 Blvd. Eroilor Sanitari, POB 15-205, RO-76241 Bucharest (Romania)
  3. Division of New Energy Conversion Methods, Institute of Research and Design for Thermoenergetic Equipment, ICPET-CERCETARE, Bucharest (Romania)
  4. Clinical Laboratory Department, Carol Davila University of Medicine and Pharmaceutics, 8 Blvd. Eroilor Sanitari, POB 15-205, RO-76241 Bucharest (Romania)
Publication Date:
Jul 01, 1999
Product Type:
Journal Article
Resource Relation:
Journal Name: Romanian Journal of Biophysics; Journal Volume: 9; Journal Issue: 1-2; Other Information: 36 refs., 2 figs., 2 tabs; PBD: 1999
Subject:
60 APPLIED LIFE SCIENCES; ACTIVATION ENERGY; BLOOD PLASMA; CELL MEMBRANES; DIABETES MELLITUS; ERYTHROCYTES; HEPATITIS; HYDROGEN 1; HYPERTENSION; MANGANESE IONS; NMR IMAGING; PERMEABILITY; REACTION KINETICS; SPIN-SPIN RELAXATION; WATER
OSTI ID:
20467510
Country of Origin:
Romania
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1220-515X; RJBEEZ; TRN: RO0410137043199
Submitting Site:
INIS
Size:
page(s) 87-97
Announcement Date:

Journal Article:

Citation Formats

Katona, Eva, Doaga, I O, Radulet, Diana, Caplanusi, A, Negreanu, Cezarina, and Mihele, Denisa. NMR studies of human blood cells in health and disease. I. Alterations of the plasma membrane water permeability of erythrocytes. Romania: N. p., 1999. Web.
Katona, Eva, Doaga, I O, Radulet, Diana, Caplanusi, A, Negreanu, Cezarina, & Mihele, Denisa. NMR studies of human blood cells in health and disease. I. Alterations of the plasma membrane water permeability of erythrocytes. Romania.
Katona, Eva, Doaga, I O, Radulet, Diana, Caplanusi, A, Negreanu, Cezarina, and Mihele, Denisa. 1999. "NMR studies of human blood cells in health and disease. I. Alterations of the plasma membrane water permeability of erythrocytes." Romania.
@misc{etde_20467510,
title = {NMR studies of human blood cells in health and disease. I. Alterations of the plasma membrane water permeability of erythrocytes}
author = {Katona, Eva, Doaga, I O, Radulet, Diana, Caplanusi, A, Negreanu, Cezarina, and Mihele, Denisa}
abstractNote = {Alterations in pathological cases of the human erythrocyte membrane water permeability were investigated by using a Mn{sup 2+}-doping {sup 1}H nuclear magnetic resonance (NMR) technique. The temperature dependence of the apparent water diffusional exchange through erythrocyte membranes in chronic hepatitis, diabetes, dyslipidemia and essential hypertension was measured and compared to healthy controls. Using moderate manganese concentrations (9-18 mM) and Carr-Purcell-Meiboom-Gill pulse sequences with a large number of refocusing {pi} pulses and short interpulse delay (100 {mu}s) our values of the water exchange times ({tau}{sub e}) across erythrocyte membranes, obtained within a 10 min time period following the moment of doping, were independent of the actual manganese concentration and the Arrhenius plot for water exchange was linear over the range of 22-42 deg C. A marked increase of the water exchange times values was observed in all studied disease states. In case of chronic hepatitis, diabetes and dyslipidemia the changes observed in transmembrane water exchange time were associated with significant increase in the apparent activation energy of the diffusional water permeability thus, pointing out alterations in the function of the erythrocyte water channel. (author)}
journal = {Romanian Journal of Biophysics}
issue = {1-2}
volume = {9}
journal type = {AC}
place = {Romania}
year = {1999}
month = {Jul}
}