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Changes induced by gamma radiation in microsomal membranes of storage of garlic; Cambios provocados por la radiacion gamma en membranas microsomales de hoja de reserva de ajo

Abstract

This study evaluates the effects of the radio inhibition process on garlic bulbs in terms of phase properties of microsomal membranes and their lipid and fatty acid composition. Garlic bulbs were irradiated with an average dose of 60 Gy of {sup 60}Co gamma rays 30-40 days after harvest. The treatment was carried out in the facilities of the National Atomic Energy Commission (CNEA). Rough and smooth microsomal membranes were isolated by ultracentrifugation from tissues of irradiated and non-irradiated storage leaves. Wide angle X-ray diffractograms of both fractions were recorded along 270 days of storage. Lipids were separated by thin layer chromatography. The fatty acid composition of major lipid fractions was studied by gas-liquid chromatography. The diffractograms featured peaks at Bragg spacing of 4.15 Armstrong and 3.75 Armstrong, revealing the presence of a gel (crystalline) phase, while the characteristic peak of the liquid-crystalline phase (4.6 Armstrong) was not observed in both sorts of membranes. Irradiation was found to bring about modifications in the intensity of 4.15 Armstrong and 3.75 Armstrong peaks from smooth microsomal membranes, but not in the behaviour along the studied period. Data from the rough microsomal fraction were erratic. Parallel to these changes, radiation induced significant modifications in  More>>
Authors:
Perez, M B; Croci, C A; [1]  Aveldano, M I [2] 
  1. Universidad Nacional del Sur, Bahia Blanca (Argentina). Laboratorio de Radioisotopos
  2. Universidad Nacional del Sur, Bahia Blanca (Argentina). Instituto de Investigaciones Bioquimicas
Publication Date:
Jul 01, 2003
Product Type:
Conference
Report Number:
INIS-AR-C-319
Resource Relation:
Conference: AATN 2001: 28. Annual meeting of the Argentine Association of Nuclear Technology, AATN 2001: 28. Reunion anual de la Asociacion Argentina de Tecnologia Nuclear (AATN), Buenos Aires (Argentina), 14-16 Nov 2001; Other Information: 12 refs., 2 figs; PBD: 2003
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL RADIATION EFFECTS; FOOD PROCESSING; GARLIC; RADIATION INDUCED MUTANTS; RADIOPRESERVATION; RADURIZATION
OSTI ID:
20403339
Country of Origin:
Argentina
Language:
Spanish
Other Identifying Numbers:
TRN: AR03C0063077952
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
4 pages
Announcement Date:
Dec 05, 2003

Citation Formats

Perez, M B, Croci, C A, and Aveldano, M I. Changes induced by gamma radiation in microsomal membranes of storage of garlic; Cambios provocados por la radiacion gamma en membranas microsomales de hoja de reserva de ajo. Argentina: N. p., 2003. Web.
Perez, M B, Croci, C A, & Aveldano, M I. Changes induced by gamma radiation in microsomal membranes of storage of garlic; Cambios provocados por la radiacion gamma en membranas microsomales de hoja de reserva de ajo. Argentina.
Perez, M B, Croci, C A, and Aveldano, M I. 2003. "Changes induced by gamma radiation in microsomal membranes of storage of garlic; Cambios provocados por la radiacion gamma en membranas microsomales de hoja de reserva de ajo." Argentina.
@misc{etde_20403339,
title = {Changes induced by gamma radiation in microsomal membranes of storage of garlic; Cambios provocados por la radiacion gamma en membranas microsomales de hoja de reserva de ajo}
author = {Perez, M B, Croci, C A, and Aveldano, M I}
abstractNote = {This study evaluates the effects of the radio inhibition process on garlic bulbs in terms of phase properties of microsomal membranes and their lipid and fatty acid composition. Garlic bulbs were irradiated with an average dose of 60 Gy of {sup 60}Co gamma rays 30-40 days after harvest. The treatment was carried out in the facilities of the National Atomic Energy Commission (CNEA). Rough and smooth microsomal membranes were isolated by ultracentrifugation from tissues of irradiated and non-irradiated storage leaves. Wide angle X-ray diffractograms of both fractions were recorded along 270 days of storage. Lipids were separated by thin layer chromatography. The fatty acid composition of major lipid fractions was studied by gas-liquid chromatography. The diffractograms featured peaks at Bragg spacing of 4.15 Armstrong and 3.75 Armstrong, revealing the presence of a gel (crystalline) phase, while the characteristic peak of the liquid-crystalline phase (4.6 Armstrong) was not observed in both sorts of membranes. Irradiation was found to bring about modifications in the intensity of 4.15 Armstrong and 3.75 Armstrong peaks from smooth microsomal membranes, but not in the behaviour along the studied period. Data from the rough microsomal fraction were erratic. Parallel to these changes, radiation induced significant modifications in the level of smooth microsomal membrane triacylglycerols in relation to phospholipids and their fatty acids. These findings indicate that the storage leaf tissues of garlic are radiosensitive both in terms of physical and chemical properties of their microsomal membranes. From the practical point of view, these results could be the basis for the development of techniques to be applied to storage garlic to evaluate if it was irradiated. (author)}
place = {Argentina}
year = {2003}
month = {Jul}
}