You need JavaScript to view this

Natural genetic transformation in Acinetobacter sp. BD413 Biofilms: introducing natural genetic transformation as a tool for bioenhancement of biofilm reactors

Abstract

This study focussed on the localization and quantification of natural genetic transformation using neutral and disadvantageous genes in monoculture biofilms to investigate gene transfer and expression of the transferred genes in the absence of a selective advantage. Data obtained by this investigation were regarded as initial steps for evaluating the applicability of adding catabolic traits into the indigenous bacterial community of biofilm reactors by in situ natural genetic transformation. Because Acinetobacter spp. strains are readily found in waste water treatment plants and because Acinetobacter sp. BD413 possesses a high effective level of competence, natural genetic transformation was investigated in monoculture Acinetobacter sp. BD413 biofilms. The genes used for transformation encoded for the green fluorescent protein (GFP) and its variants. Monitoring of transformation events were performed with the use of automated confocal laser scanning microscopy (CLSM) and semi automated digital image processing and analysis. (orig.)
Authors:
Publication Date:
Jul 01, 2002
Product Type:
Miscellaneous
Report Number:
ETDE-DE-1442
Resource Relation:
Other Information: PBD: 2002; Related Information: Berichte aus Wasserguete- und Abfallwirtschaft der Technischen Universitaet Muenchenv. 171
Subject:
60 APPLIED LIFE SCIENCES; WATER TREATMENT; FILMS; BACTERIA; GENETIC VARIABILITY; GENE MUTATIONS; GENETIC ENGINEERING; BIOREACTORS; BIOLOGICAL MARKERS; FLUORESCENCE
OSTI ID:
20345688
Research Organizations:
Gesellschaft zur Foerderung des Lehrstuhls fuer Wasserguete und Abfallwirtschaft der Technischen Univ. Muenchen e.V., Garching (Germany)
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ISSN 0942-914X; TRN: DE03G5798
Availability:
Available to ETDE participating countries only(see www.etde.org); commercial reproduction prohibited; OSTI as DE20345688
Submitting Site:
DE
Size:
192 pages
Announcement Date:

Citation Formats

Hendrickx, L. Natural genetic transformation in Acinetobacter sp. BD413 Biofilms: introducing natural genetic transformation as a tool for bioenhancement of biofilm reactors. Germany: N. p., 2002. Web.
Hendrickx, L. Natural genetic transformation in Acinetobacter sp. BD413 Biofilms: introducing natural genetic transformation as a tool for bioenhancement of biofilm reactors. Germany.
Hendrickx, L. 2002. "Natural genetic transformation in Acinetobacter sp. BD413 Biofilms: introducing natural genetic transformation as a tool for bioenhancement of biofilm reactors." Germany.
@misc{etde_20345688,
title = {Natural genetic transformation in Acinetobacter sp. BD413 Biofilms: introducing natural genetic transformation as a tool for bioenhancement of biofilm reactors}
author = {Hendrickx, L}
abstractNote = {This study focussed on the localization and quantification of natural genetic transformation using neutral and disadvantageous genes in monoculture biofilms to investigate gene transfer and expression of the transferred genes in the absence of a selective advantage. Data obtained by this investigation were regarded as initial steps for evaluating the applicability of adding catabolic traits into the indigenous bacterial community of biofilm reactors by in situ natural genetic transformation. Because Acinetobacter spp. strains are readily found in waste water treatment plants and because Acinetobacter sp. BD413 possesses a high effective level of competence, natural genetic transformation was investigated in monoculture Acinetobacter sp. BD413 biofilms. The genes used for transformation encoded for the green fluorescent protein (GFP) and its variants. Monitoring of transformation events were performed with the use of automated confocal laser scanning microscopy (CLSM) and semi automated digital image processing and analysis. (orig.)}
place = {Germany}
year = {2002}
month = {Jul}
}