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Genetic diversity analysis in rice mutants using isozyme and Morphological markers

Abstract

In this work, isozyme and agromorphologic variability of radiation-induced rice mutants with different cytoplasm base was surveyed. Agromorphologic data (plant type, lodging resistance, life cycle and yielding) were transformed into binary data. This markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidase) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation. Genetic Similarity between individuals was obtained based on Dice's Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different cytoplasm sources. However, further discrimination between control varieties and their mutants could be obtained. Bootstrapping analysis was performed to estimate the robustness of the group in the phenogram. According to their bootstrap P value (99.6%), Basmati-370 mutant lines could be considered statistically different from their control. This analysis is suggested as an useful supporting tool for an accurate varietal validation. A Multiple Correspondence Analysis (MCA) showed individuals dispersion around the three principal axis of variation. In general the UPGMA phenogram pattern was corroborated at MCA. Variables such as life cycle, presence of bands Est-a and Prx-m and the absence of Est-i, Prx-h and Prx-i accounted for the higher contribution to variation. The adequacy of  More>>
Authors:
Fuentes, Jorge L; Alvarez, Alba; [1]  Deus, Juan E; [2]  Duque, Miriam C; [3]  Cornide, Maria T [4] 
  1. Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba)
  2. Instituto de Investigaciones del Arroz. Bauta, La Habana (Cuba)
  3. Centro Internacional de Agricultura Tropical, Cali (Colombia)
  4. Centro Nacional de Investigaciones Cientificas, La Habana (Cuba)
Publication Date:
Jul 01, 1999
Product Type:
Conference
Resource Relation:
Conference: 2. NURT 1999, La Habana (Cuba), 26-29 Oct 1999; Other Information: Published only in CD-ROM; 8 p; PBD: 1999
Subject:
60 APPLIED LIFE SCIENCES; AGRICULTURE; GENETIC VARIABILITY; PLANT BREEDING; RADIATION INDUCED MUTANTS; RICE
OSTI ID:
20279451
Research Organizations:
International Atomic Energy Agency, Viena (Austria); Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba); Agencia de Energia Nuclear, La Habana (Cuba); Instituto Superior de Ciencia y Tecnologia Nuclear, La Habana (Cuba); Centro de Informacion de la energia Nuclear, La Habana (Cuba); Centro de Tecnologia Nuclear, La Habana (Cuba); Instituto de Investigaciones Fundamentales de la Agricultura Tropical, La Habana (Cuba)
Country of Origin:
Cuba
Language:
English
Other Identifying Numbers:
TRN: CU0200027041822
Availability:
Available from the library of the CIEN E-mail: katia@cien.energia.inf.cu; belkis@cien.energia.inf.cu;INIS
Submitting Site:
INIS
Size:
CD-ROM pages
Announcement Date:
Oct 16, 2002

Citation Formats

Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T. Genetic diversity analysis in rice mutants using isozyme and Morphological markers. Cuba: N. p., 1999. Web.
Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, & Cornide, Maria T. Genetic diversity analysis in rice mutants using isozyme and Morphological markers. Cuba.
Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T. 1999. "Genetic diversity analysis in rice mutants using isozyme and Morphological markers." Cuba.
@misc{etde_20279451,
title = {Genetic diversity analysis in rice mutants using isozyme and Morphological markers}
author = {Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T}
abstractNote = {In this work, isozyme and agromorphologic variability of radiation-induced rice mutants with different cytoplasm base was surveyed. Agromorphologic data (plant type, lodging resistance, life cycle and yielding) were transformed into binary data. This markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidase) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation. Genetic Similarity between individuals was obtained based on Dice's Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different cytoplasm sources. However, further discrimination between control varieties and their mutants could be obtained. Bootstrapping analysis was performed to estimate the robustness of the group in the phenogram. According to their bootstrap P value (99.6%), Basmati-370 mutant lines could be considered statistically different from their control. This analysis is suggested as an useful supporting tool for an accurate varietal validation. A Multiple Correspondence Analysis (MCA) showed individuals dispersion around the three principal axis of variation. In general the UPGMA phenogram pattern was corroborated at MCA. Variables such as life cycle, presence of bands Est-a and Prx-m and the absence of Est-i, Prx-h and Prx-i accounted for the higher contribution to variation. The adequacy of morphological and isozyme descriptors for new mutant lines validation is also discussed.}
place = {Cuba}
year = {1999}
month = {Jul}
}