Abstract
In this work, isozyme and agromorphologic variability of radiation-induced rice mutants with different cytoplasm base was surveyed. Agromorphologic data (plant type, lodging resistance, life cycle and yielding) were transformed into binary data. This markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidase) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation. Genetic Similarity between individuals was obtained based on Dice's Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different cytoplasm sources. However, further discrimination between control varieties and their mutants could be obtained. Bootstrapping analysis was performed to estimate the robustness of the group in the phenogram. According to their bootstrap P value (99.6%), Basmati-370 mutant lines could be considered statistically different from their control. This analysis is suggested as an useful supporting tool for an accurate varietal validation. A Multiple Correspondence Analysis (MCA) showed individuals dispersion around the three principal axis of variation. In general the UPGMA phenogram pattern was corroborated at MCA. Variables such as life cycle, presence of bands Est-a and Prx-m and the absence of Est-i, Prx-h and Prx-i accounted for the higher contribution to variation. The adequacy of
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Fuentes, Jorge L;
Alvarez, Alba;
[1]
Deus, Juan E;
[2]
Duque, Miriam C;
[3]
Cornide, Maria T
[4]
- Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba)
- Instituto de Investigaciones del Arroz. Bauta, La Habana (Cuba)
- Centro Internacional de Agricultura Tropical, Cali (Colombia)
- Centro Nacional de Investigaciones Cientificas, La Habana (Cuba)
Citation Formats
Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T.
Genetic diversity analysis in rice mutants using isozyme and Morphological markers.
Cuba: N. p.,
1999.
Web.
Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, & Cornide, Maria T.
Genetic diversity analysis in rice mutants using isozyme and Morphological markers.
Cuba.
Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T.
1999.
"Genetic diversity analysis in rice mutants using isozyme and Morphological markers."
Cuba.
@misc{etde_20279451,
title = {Genetic diversity analysis in rice mutants using isozyme and Morphological markers}
author = {Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T}
abstractNote = {In this work, isozyme and agromorphologic variability of radiation-induced rice mutants with different cytoplasm base was surveyed. Agromorphologic data (plant type, lodging resistance, life cycle and yielding) were transformed into binary data. This markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidase) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation. Genetic Similarity between individuals was obtained based on Dice's Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different cytoplasm sources. However, further discrimination between control varieties and their mutants could be obtained. Bootstrapping analysis was performed to estimate the robustness of the group in the phenogram. According to their bootstrap P value (99.6%), Basmati-370 mutant lines could be considered statistically different from their control. This analysis is suggested as an useful supporting tool for an accurate varietal validation. A Multiple Correspondence Analysis (MCA) showed individuals dispersion around the three principal axis of variation. In general the UPGMA phenogram pattern was corroborated at MCA. Variables such as life cycle, presence of bands Est-a and Prx-m and the absence of Est-i, Prx-h and Prx-i accounted for the higher contribution to variation. The adequacy of morphological and isozyme descriptors for new mutant lines validation is also discussed.}
place = {Cuba}
year = {1999}
month = {Jul}
}
title = {Genetic diversity analysis in rice mutants using isozyme and Morphological markers}
author = {Fuentes, Jorge L, Alvarez, Alba, Deus, Juan E, Duque, Miriam C, and Cornide, Maria T}
abstractNote = {In this work, isozyme and agromorphologic variability of radiation-induced rice mutants with different cytoplasm base was surveyed. Agromorphologic data (plant type, lodging resistance, life cycle and yielding) were transformed into binary data. This markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidase) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation. Genetic Similarity between individuals was obtained based on Dice's Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different cytoplasm sources. However, further discrimination between control varieties and their mutants could be obtained. Bootstrapping analysis was performed to estimate the robustness of the group in the phenogram. According to their bootstrap P value (99.6%), Basmati-370 mutant lines could be considered statistically different from their control. This analysis is suggested as an useful supporting tool for an accurate varietal validation. A Multiple Correspondence Analysis (MCA) showed individuals dispersion around the three principal axis of variation. In general the UPGMA phenogram pattern was corroborated at MCA. Variables such as life cycle, presence of bands Est-a and Prx-m and the absence of Est-i, Prx-h and Prx-i accounted for the higher contribution to variation. The adequacy of morphological and isozyme descriptors for new mutant lines validation is also discussed.}
place = {Cuba}
year = {1999}
month = {Jul}
}