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Raps markers for genetic diversity analysis in rice (Oryza sativa L)

Abstract

The establishment of relationships between genotypes existing in gene banks that may be used in new crosses, and about genetic diversity in available germplasm, is very useful for plant breeders. In this work, a genetic diversity analysis among 20 varieties of the Cuban rice germplasm bank was performed by using RAPD markers. Twenty four decamer primers were screened which produced 61 polymorphic bands out of 105 consistent and reproducible amplified fragments (58.1 %). The proportion of polymorphic bands varied for each primer, with an average of 3 polymorphic bands per primer, these results agreed with previous reports on RAPD polymorphism in rice germplasm. Depending on the primer, 1 to 7 distinct patterns were obtained among the screened genotypes. Pair-wise genetic distances between genotypes were computed based on Dice's coefficient. Three major, statistically robust groups were obtained in the UPGMA dendrogram (A, B and C) which clearly corresponded to different genetic pools. Additionally, more insight could be gained according to the sub-grouping pattern within group A, which included the principal semi-dwarf commercial varieties. The present study allowed to prove the efficiency of RAPD markers for genetic diversity analysis in closely related germplasm, particularly for the semi-dwarf Cuban commercial rice cultivars. Also,  More>>
Authors:
Alvarez, A; Fuentes, Jorge L; [1]  Deus, Juan E; [2]  Duque, Maria C [3] 
  1. Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba)
  2. Instituto de Investigaciones del Arroz, Habana (Cuba)
  3. Centro Internacional de la Agricultura Tropical. Proyecto de Arroz , Cali (Colombia)
Publication Date:
Jul 01, 1999
Product Type:
Conference
Resource Relation:
Conference: 2. NURT 1999, La Habana (Cuba), 26-29 Oct 1999; Other Information: Published only in CD-ROM; 11 p; PBD: 1999
Subject:
60 APPLIED LIFE SCIENCES; AGRICULTURE; BIOLOGICAL MARKERS; BIOLOGICAL STRESS; COORDINATED RESEARCH PROGRAMS; DNA; GENETIC VARIABILITY; PLANT BREEDING; RADIATION INDUCED MUTANTS; RFLPS; RICE; TRACER TECHNIQUES
OSTI ID:
20279445
Research Organizations:
International Atomic Energy Agency, Viena (Austria); Centro de Estudios Aplicados al Desarrollo Nuclear, La Habana (Cuba); Agencia de Energia Nuclear, La Habana (Cuba); Instituto Superior de Ciencia y Tecnologia Nuclear, La Habana (Cuba); Centro de Informacion de la Energia Nuclear, La Habana (Cuba); Centro de Tecnologia Nuclear, La Habana (Cuba); Instituto de Investigaciones Fundamentales de la Agricultura Tropical, La Habana (Cuba)
Country of Origin:
Cuba
Language:
English
Other Identifying Numbers:
TRN: CU0200021041816
Availability:
Available from the library of the CIEN E-mail: katia@cien.energia.inf.cu; belkis@cien.energia.inf.cu;INIS
Submitting Site:
INIS
Size:
CD-ROM pages
Announcement Date:

Citation Formats

Alvarez, A, Fuentes, Jorge L, Deus, Juan E, and Duque, Maria C. Raps markers for genetic diversity analysis in rice (Oryza sativa L). Cuba: N. p., 1999. Web.
Alvarez, A, Fuentes, Jorge L, Deus, Juan E, & Duque, Maria C. Raps markers for genetic diversity analysis in rice (Oryza sativa L). Cuba.
Alvarez, A, Fuentes, Jorge L, Deus, Juan E, and Duque, Maria C. 1999. "Raps markers for genetic diversity analysis in rice (Oryza sativa L)." Cuba.
@misc{etde_20279445,
title = {Raps markers for genetic diversity analysis in rice (Oryza sativa L)}
author = {Alvarez, A, Fuentes, Jorge L, Deus, Juan E, and Duque, Maria C}
abstractNote = {The establishment of relationships between genotypes existing in gene banks that may be used in new crosses, and about genetic diversity in available germplasm, is very useful for plant breeders. In this work, a genetic diversity analysis among 20 varieties of the Cuban rice germplasm bank was performed by using RAPD markers. Twenty four decamer primers were screened which produced 61 polymorphic bands out of 105 consistent and reproducible amplified fragments (58.1 %). The proportion of polymorphic bands varied for each primer, with an average of 3 polymorphic bands per primer, these results agreed with previous reports on RAPD polymorphism in rice germplasm. Depending on the primer, 1 to 7 distinct patterns were obtained among the screened genotypes. Pair-wise genetic distances between genotypes were computed based on Dice's coefficient. Three major, statistically robust groups were obtained in the UPGMA dendrogram (A, B and C) which clearly corresponded to different genetic pools. Additionally, more insight could be gained according to the sub-grouping pattern within group A, which included the principal semi-dwarf commercial varieties. The present study allowed to prove the efficiency of RAPD markers for genetic diversity analysis in closely related germplasm, particularly for the semi-dwarf Cuban commercial rice cultivars. Also, the existence of a narrow genetic base among these varieties has been confirmed, pointing at the urgent necessity of widen it.}
place = {Cuba}
year = {1999}
month = {Jul}
}