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Study of embryonic ploidy: a probable embryo model

Abstract

The second polar body (PB) studies in preimplantation mouse embryos were carried out to evaluate the possibility as reference cell to analyze ploidy. For that purpose embryos in a one cell stage [obtained by crossing hybrid females (CBAxC57BL) to NIH males] were cultured in vitro during 72 hs, individually fixed at morula stage and stained with Feulgen. The DNA content of 263 individual nucleus was evaluated cytophotometrically corresponding to 22 compact morulas of normal development. As haploid PB is present in all pre implanted stage, only embryos with one haploid nuclei were considered as normal. In 95.5% (n = 21) of the embryos the PB was present. DNA measurement of 21 PB was 1n {+-} 0.1. By the height sensibility of PB ploidy, the abnormalities were detected by the criterion of >4.1 n and <1.9 n. The results showed that one embryo was completely haploid (1n). The rest of the embryos (n = 20) 222 blastomeres and 20 PB were analyzed. The DNA measurement showed that 92,7% of the blastomeres (n = 206) are between 2 n and 4 n and 7.3% showed ploidy anomalies, regarding the value n of their PB. The period of the cellular cycle was studied  More>>
Authors:
Kundt, Miriam S; Cabrini, Romulo L [1] 
  1. Comision Nacional de Energia Atomica, Buenos Aires (Argentina). Dept. de Radiobiologia
Publication Date:
Jul 01, 2001
Product Type:
Conference
Report Number:
INIS-AR-C-187
Resource Relation:
Conference: Congress on 'in vitro' biology, St. Louis, MO (United States), 16-20 Jun 2001; Other Information: Pre-conference paper; 3 refs., 4 figs; PBD: 2001
Subject:
60 APPLIED LIFE SCIENCES; CELL CULTURES; CELL CYCLE; CHROMOSOMAL ABERRATIONS; DNA; EMBRYONIC CELLS; EMBRYOS; FERTILIZATION; GENOME MUTATIONS; HAPLOIDY; IN VITRO; RATS
OSTI ID:
20273609
Research Organizations:
Comision Nacional de Energia Atomica, Buenos Aires (Argentina)
Country of Origin:
Argentina
Language:
English
Other Identifying Numbers:
TRN: AR02C0002037916
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
5 pages
Announcement Date:
Sep 12, 2002

Citation Formats

Kundt, Miriam S, and Cabrini, Romulo L. Study of embryonic ploidy: a probable embryo model. Argentina: N. p., 2001. Web.
Kundt, Miriam S, &amp; Cabrini, Romulo L. Study of embryonic ploidy: a probable embryo model. Argentina.
Kundt, Miriam S, and Cabrini, Romulo L. 2001. "Study of embryonic ploidy: a probable embryo model." Argentina.
@misc{etde_20273609,
title = {Study of embryonic ploidy: a probable embryo model}
author = {Kundt, Miriam S, and Cabrini, Romulo L}
abstractNote = {The second polar body (PB) studies in preimplantation mouse embryos were carried out to evaluate the possibility as reference cell to analyze ploidy. For that purpose embryos in a one cell stage [obtained by crossing hybrid females (CBAxC57BL) to NIH males] were cultured in vitro during 72 hs, individually fixed at morula stage and stained with Feulgen. The DNA content of 263 individual nucleus was evaluated cytophotometrically corresponding to 22 compact morulas of normal development. As haploid PB is present in all pre implanted stage, only embryos with one haploid nuclei were considered as normal. In 95.5% (n = 21) of the embryos the PB was present. DNA measurement of 21 PB was 1n {+-} 0.1. By the height sensibility of PB ploidy, the abnormalities were detected by the criterion of >4.1 n and <1.9 n. The results showed that one embryo was completely haploid (1n). The rest of the embryos (n = 20) 222 blastomeres and 20 PB were analyzed. The DNA measurement showed that 92,7% of the blastomeres (n = 206) are between 2 n and 4 n and 7.3% showed ploidy anomalies, regarding the value n of their PB. The period of the cellular cycle was studied in the normal cell ploidy. This study showed that 16.5% of the blastomeres (n = 34) were in the period G1, 70.39% (n =34) in the period S and 13.2% in the period G2 (n = 27). It is concluded that the PB study showed that it has properties as an excellent indicator of internal ploidia: it is present from the moment of the conception, easily recognizable in the perivitelin space in the embryo of one-two cells, remains in interface during the preimplantation development, it is haploid and digitalized pixel by pixel PB study showed the homogeneity of this type of cell, giving a reliable value of ploidy. The properties of the PB and the results showed that the PB could be an excellent indicator for embryonic ploidy studies on genotoxicity, maintaining its original ploidia during the preimplantation development while the blastomeres are susceptible to changes in its content of DNA starting from the first embryonic cleavage. (author)}
place = {Argentina}
year = {2001}
month = {Jul}
}