You need JavaScript to view this

Induction of male sterility in rice using chemical mutagens

Abstract

Full text: To diversify the sources of cytoplasmic male sterility for hybrid seed production in rice (Oryza sativa L.) attempts were made to induce this character in a popular indica cultivar PR 106 through chemical mutagens. Seeds were treated with 0.4% ethidium bromide (EB) for 24 or 48h at 10 deg. C, with 0.4% ethyl methanesulphonate (EMS) for 24 or 48h at 10 deg. C for 16 hr at 20 deg. C or with 0.2% streptomycin sulphate (SM) for 24 or 48 hr at 10 deg. C. In M{sub 2} male sterile plants were detected in eleven different progenies, one from SM treatment and the remaining from EMS treatments. All the sterile plants had 100% non-stainable aborted pollen. Seed set upon open-pollination of the male sterile plants with the variety PR 106 ranged from 0.03 to 4.93 per cent whereas no seed formed in bagged panicles. In M{sub 3}, open-pollinated progenies of the male sterile plants and their fertile sibs were further studied. Two progenies segregated for male sterility, all others had only fertile plants. In one of the segregating progenies, five out of six and in the other nine out of fourteen plants were male sterile. The progenies of  More>>
Authors:
Minocha, J L; Gupta, R K [1] 
  1. Department of Genetics, Punjab Agricultural University, Ludhiana (India)
Publication Date:
Jul 01, 1988
Product Type:
Technical Report
Report Number:
INIS-XA-480
Resource Relation:
Other Information: PBD: Jul 1988; Related Information: In: Mutation breeding newsletter. No. 32, 36 pages.
Subject:
60 APPLIED LIFE SCIENCES; GENETIC VARIABILITY; MALES; PLANT BREEDING; RADIATION INDUCED MUTANTS; RADIOSTERILIZATION; RICE; SEEDS
OSTI ID:
20244504
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 1011-260X; TRN: XA0201324017208
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 5-6
Announcement Date:

Citation Formats

Minocha, J L, and Gupta, R K. Induction of male sterility in rice using chemical mutagens. IAEA: N. p., 1988. Web.
Minocha, J L, & Gupta, R K. Induction of male sterility in rice using chemical mutagens. IAEA.
Minocha, J L, and Gupta, R K. 1988. "Induction of male sterility in rice using chemical mutagens." IAEA.
@misc{etde_20244504,
title = {Induction of male sterility in rice using chemical mutagens}
author = {Minocha, J L, and Gupta, R K}
abstractNote = {Full text: To diversify the sources of cytoplasmic male sterility for hybrid seed production in rice (Oryza sativa L.) attempts were made to induce this character in a popular indica cultivar PR 106 through chemical mutagens. Seeds were treated with 0.4% ethidium bromide (EB) for 24 or 48h at 10 deg. C, with 0.4% ethyl methanesulphonate (EMS) for 24 or 48h at 10 deg. C for 16 hr at 20 deg. C or with 0.2% streptomycin sulphate (SM) for 24 or 48 hr at 10 deg. C. In M{sub 2} male sterile plants were detected in eleven different progenies, one from SM treatment and the remaining from EMS treatments. All the sterile plants had 100% non-stainable aborted pollen. Seed set upon open-pollination of the male sterile plants with the variety PR 106 ranged from 0.03 to 4.93 per cent whereas no seed formed in bagged panicles. In M{sub 3}, open-pollinated progenies of the male sterile plants and their fertile sibs were further studied. Two progenies segregated for male sterility, all others had only fertile plants. In one of the segregating progenies, five out of six and in the other nine out of fourteen plants were male sterile. The progenies of fertile sibs did not have any male sterile plant. The results indicate that sterility of cytoplasmic type has been induced by EMS. The parental variety PR 106 acts as the maintainer. (author)}
place = {IAEA}
year = {1988}
month = {Jul}
}