Abstract
Objective: To study the effects of {gamma}-irradiation on cell cycle and proliferation of fibroblasts and the radioprotective effect of PGL in term of cellular level. Methods: The cells in the control group and PGL (50 mg/L. 100 mg/L. 150 mg/L) groups were irradiated with 15 Gy {gamma}-rays. The cell cycle and proliferation of NIH3T3 cells were determined by flow cytometry after irradiation within 12 h. Results: The percentage of G{sub 0}-G{sub 1} phase was 52.76% in the positive control group. Compared with the negative control group (90.9%), the proliferation of NIH3T3 was promoted significantly (P < 0.01). The percentages of G{sub 0}-G{sub 1} phases in the GPL (50 mg/L, 100 mg/L, 150 mg/L) groups were 66.17%, 71.73%, 76.10% respectively, the proliferation of NIH3T3 cells was inhibited by adding GPL. compared with the positive control group, in the 50 mg/L group P < 0.05, in the 100 mg/L and 150 mg/L groups P < 0.01. It was found that the apoptosis of NIH3T3 cells had little change after comparing non-irradiated group with the irradiated groups. Conclusion: Radiation with {gamma}-rays at a dose of 15 Gy, can significantly stimulate proliferation of NIH3T3 cells, but no induced apoptosis can not be achieve within
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Xiuqing, Ji;
Shiling, Wu;
Yinghui, Zhou;
Lan, Xu
[1]
- Suzhou Univ. (China). Dept. of Biochemistry and Molecular
Citation Formats
Xiuqing, Ji, Shiling, Wu, Yinghui, Zhou, and Lan, Xu.
Effect of polysaccharides of ganoderma lucidum (PGL) on cell cycle and proliferation of NIH3T3 fibroblast exposed to {gamma}-rays.
China: N. p.,
2001.
Web.
Xiuqing, Ji, Shiling, Wu, Yinghui, Zhou, & Lan, Xu.
Effect of polysaccharides of ganoderma lucidum (PGL) on cell cycle and proliferation of NIH3T3 fibroblast exposed to {gamma}-rays.
China.
Xiuqing, Ji, Shiling, Wu, Yinghui, Zhou, and Lan, Xu.
2001.
"Effect of polysaccharides of ganoderma lucidum (PGL) on cell cycle and proliferation of NIH3T3 fibroblast exposed to {gamma}-rays."
China.
@misc{etde_20243111,
title = {Effect of polysaccharides of ganoderma lucidum (PGL) on cell cycle and proliferation of NIH3T3 fibroblast exposed to {gamma}-rays}
author = {Xiuqing, Ji, Shiling, Wu, Yinghui, Zhou, and Lan, Xu}
abstractNote = {Objective: To study the effects of {gamma}-irradiation on cell cycle and proliferation of fibroblasts and the radioprotective effect of PGL in term of cellular level. Methods: The cells in the control group and PGL (50 mg/L. 100 mg/L. 150 mg/L) groups were irradiated with 15 Gy {gamma}-rays. The cell cycle and proliferation of NIH3T3 cells were determined by flow cytometry after irradiation within 12 h. Results: The percentage of G{sub 0}-G{sub 1} phase was 52.76% in the positive control group. Compared with the negative control group (90.9%), the proliferation of NIH3T3 was promoted significantly (P < 0.01). The percentages of G{sub 0}-G{sub 1} phases in the GPL (50 mg/L, 100 mg/L, 150 mg/L) groups were 66.17%, 71.73%, 76.10% respectively, the proliferation of NIH3T3 cells was inhibited by adding GPL. compared with the positive control group, in the 50 mg/L group P < 0.05, in the 100 mg/L and 150 mg/L groups P < 0.01. It was found that the apoptosis of NIH3T3 cells had little change after comparing non-irradiated group with the irradiated groups. Conclusion: Radiation with {gamma}-rays at a dose of 15 Gy, can significantly stimulate proliferation of NIH3T3 cells, but no induced apoptosis can not be achieve within 12 h after irradiation. PGL can inhibit cell proliferation, so PGL has radioprotective activity on cell levels.}
journal = []
issue = {4}
volume = {21}
journal type = {AC}
place = {China}
year = {2001}
month = {Jul}
}
title = {Effect of polysaccharides of ganoderma lucidum (PGL) on cell cycle and proliferation of NIH3T3 fibroblast exposed to {gamma}-rays}
author = {Xiuqing, Ji, Shiling, Wu, Yinghui, Zhou, and Lan, Xu}
abstractNote = {Objective: To study the effects of {gamma}-irradiation on cell cycle and proliferation of fibroblasts and the radioprotective effect of PGL in term of cellular level. Methods: The cells in the control group and PGL (50 mg/L. 100 mg/L. 150 mg/L) groups were irradiated with 15 Gy {gamma}-rays. The cell cycle and proliferation of NIH3T3 cells were determined by flow cytometry after irradiation within 12 h. Results: The percentage of G{sub 0}-G{sub 1} phase was 52.76% in the positive control group. Compared with the negative control group (90.9%), the proliferation of NIH3T3 was promoted significantly (P < 0.01). The percentages of G{sub 0}-G{sub 1} phases in the GPL (50 mg/L, 100 mg/L, 150 mg/L) groups were 66.17%, 71.73%, 76.10% respectively, the proliferation of NIH3T3 cells was inhibited by adding GPL. compared with the positive control group, in the 50 mg/L group P < 0.05, in the 100 mg/L and 150 mg/L groups P < 0.01. It was found that the apoptosis of NIH3T3 cells had little change after comparing non-irradiated group with the irradiated groups. Conclusion: Radiation with {gamma}-rays at a dose of 15 Gy, can significantly stimulate proliferation of NIH3T3 cells, but no induced apoptosis can not be achieve within 12 h after irradiation. PGL can inhibit cell proliferation, so PGL has radioprotective activity on cell levels.}
journal = []
issue = {4}
volume = {21}
journal type = {AC}
place = {China}
year = {2001}
month = {Jul}
}