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Radiation-induced mutations in sweet cherry (Prunus avium L.)

Abstract

Full text: Dormant scions of 'Bing' were exposed to 1-2.5 kR gamma radiation. The main buds were excised and the scions grafted to allow the growth of accessory buds into primary shoots. The frequency and types of mutations were described in a population of 3307 M{sub 1}V{sub 2} shoot. The overall mutation frequency was 2.7% incl. 0.15% growth-reduced mutants. The experiment was repeated using 3kR and 4kR fractionated doses in water. Differences in mutation frequency at 3kR and 4kR were not significant. Of 2765 surviving M{sub 1}V{sub 2} shoots derived from irradiation of accessory buds of both standard and V{sub 1} shoots, the overall mutation frequency was 3.3% incl. 1.7% partial leaf mutants, 1.0% leaf mutants, and 0.54% growth-reduced mutants. For maximum mutation rate with adequate survival we suggest acute irradiation of accessory buds in air at dosages approximating LD50. Mutant sectors in M{sub 1}V{sub 1} shoots derived from accessory buds are larger than those from main buds, as revealed by the higher number of mutant repeats. (author)
Authors:
Saamin, S; [1]  Thompson, M M [2] 
  1. Cocoa and Coconut Research Division, Malaysian Agricultural Research and Development Institute (Malaysia)
  2. Department of Horticulture, Oregon State University, Corvallis, OR (United States)
Publication Date:
Jan 01, 1989
Product Type:
Conference
Report Number:
INIS-XA-478
Resource Relation:
Conference: SABRAO international symposium and workshop on gene manipulation for plant improvement in developing countries, Kuala Lumpur (Malaysia), 30 Nov - 3 Dec 1987; Other Information: PBD: Jan 1989; Related Information: In: Mutation breeding newsletter. No. 33, 36 pages.
Subject:
60 APPLIED LIFE SCIENCES; ACUTE IRRADIATION; BUDS; CHERRIES; GENETIC VARIABILITY; MUTAGEN SCREENING; MUTATION FREQUENCY; RADIATION INDUCED MUTANTS; ROSACEAE
OSTI ID:
20241770
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 1011-260X; TRN: XA0201026015353
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 8-9
Announcement Date:
Apr 30, 2002

Citation Formats

Saamin, S, and Thompson, M M. Radiation-induced mutations in sweet cherry (Prunus avium L.). IAEA: N. p., 1989. Web.
Saamin, S, & Thompson, M M. Radiation-induced mutations in sweet cherry (Prunus avium L.). IAEA.
Saamin, S, and Thompson, M M. 1989. "Radiation-induced mutations in sweet cherry (Prunus avium L.)." IAEA.
@misc{etde_20241770,
title = {Radiation-induced mutations in sweet cherry (Prunus avium L.)}
author = {Saamin, S, and Thompson, M M}
abstractNote = {Full text: Dormant scions of 'Bing' were exposed to 1-2.5 kR gamma radiation. The main buds were excised and the scions grafted to allow the growth of accessory buds into primary shoots. The frequency and types of mutations were described in a population of 3307 M{sub 1}V{sub 2} shoot. The overall mutation frequency was 2.7% incl. 0.15% growth-reduced mutants. The experiment was repeated using 3kR and 4kR fractionated doses in water. Differences in mutation frequency at 3kR and 4kR were not significant. Of 2765 surviving M{sub 1}V{sub 2} shoots derived from irradiation of accessory buds of both standard and V{sub 1} shoots, the overall mutation frequency was 3.3% incl. 1.7% partial leaf mutants, 1.0% leaf mutants, and 0.54% growth-reduced mutants. For maximum mutation rate with adequate survival we suggest acute irradiation of accessory buds in air at dosages approximating LD50. Mutant sectors in M{sub 1}V{sub 1} shoots derived from accessory buds are larger than those from main buds, as revealed by the higher number of mutant repeats. (author)}
place = {IAEA}
year = {1989}
month = {Jan}
}