Abstract
The powdered culture media (RPMI - 1640) were reconstituted either with normal distilled water (150 ppm deuterium) either with deuterium - depleted water (DDW) in various concentrations (30, 60, 90 ppm) and sterilized by filtration with 0.2 {mu}m filters. The cell lines used were NIH (normal mouse fibroblasts), RAG (mouse renal carcinoma) and TS/A (mouse mammary adenocarcinoma). In auxiliary tests, BAIBC mouse splenocytes in direct culture were used, stimulated for growth with concanavalin A or LPS (bacterial lipopolysaccharide). The estimation of the growth was made using the MTT assay or direct counting with trypan blue exclusion. The following results were obtained: Deuterium - depleted water had a stimulating effect on cell growth, the most important stimulating action being from the 90 ppm deuterium-water. The growth curves show, in a first phase, a stimulation of the rapid -growing neoplastic cells, followed by a slower growth of the normal cells. Amiloride 100 mM blocking of the Na{sup +}/K{sup +} membrane pump did not affect the cell growth curves, while the lansoprazole 100 mM blocking of the K{sup +}/H{sup +} ATP-ase brought the growth curves at the level of those with normal water. This might show an eventual involvement of the K{sup +}/H{sup
More>>
Bild, W;
[1]
Schuller, T;
Zhihai, Qin;
Blankenstein, T;
[2]
Nastasa, V;
Haulica, I
[3]
- University of Medicine and Farmacy, UMF, Str. Universitatii 16, RO-6600 Iasi (Romania)
- Immunology Department, Max Delbruck Centrum Berlin, Berlin (Germany)
- Laboratory of Experimental and Applied Physiology of the Romanian Academy, Calea Victoriei 125, Sect. 1 Bucharest (Romania)
Citation Formats
Bild, W, Schuller, T, Zhihai, Qin, Blankenstein, T, Nastasa, V, and Haulica, I.
The effects of environmental deuterium on normal and neoplastic cultured cell development.
Romania: N. p.,
2000.
Web.
Bild, W, Schuller, T, Zhihai, Qin, Blankenstein, T, Nastasa, V, & Haulica, I.
The effects of environmental deuterium on normal and neoplastic cultured cell development.
Romania.
Bild, W, Schuller, T, Zhihai, Qin, Blankenstein, T, Nastasa, V, and Haulica, I.
2000.
"The effects of environmental deuterium on normal and neoplastic cultured cell development."
Romania.
@misc{etde_20240485,
title = {The effects of environmental deuterium on normal and neoplastic cultured cell development}
author = {Bild, W, Schuller, T, Zhihai, Qin, Blankenstein, T, Nastasa, V, and Haulica, I}
abstractNote = {The powdered culture media (RPMI - 1640) were reconstituted either with normal distilled water (150 ppm deuterium) either with deuterium - depleted water (DDW) in various concentrations (30, 60, 90 ppm) and sterilized by filtration with 0.2 {mu}m filters. The cell lines used were NIH (normal mouse fibroblasts), RAG (mouse renal carcinoma) and TS/A (mouse mammary adenocarcinoma). In auxiliary tests, BAIBC mouse splenocytes in direct culture were used, stimulated for growth with concanavalin A or LPS (bacterial lipopolysaccharide). The estimation of the growth was made using the MTT assay or direct counting with trypan blue exclusion. The following results were obtained: Deuterium - depleted water had a stimulating effect on cell growth, the most important stimulating action being from the 90 ppm deuterium-water. The growth curves show, in a first phase, a stimulation of the rapid -growing neoplastic cells, followed by a slower growth of the normal cells. Amiloride 100 mM blocking of the Na{sup +}/K{sup +} membrane pump did not affect the cell growth curves, while the lansoprazole 100 mM blocking of the K{sup +}/H{sup +} ATP-ase brought the growth curves at the level of those with normal water. This might show an eventual involvement of the K{sup +}/H{sup +} antiport in the stimulating effects of the DDW. (authors)}
place = {Romania}
year = {2000}
month = {Jul}
}
title = {The effects of environmental deuterium on normal and neoplastic cultured cell development}
author = {Bild, W, Schuller, T, Zhihai, Qin, Blankenstein, T, Nastasa, V, and Haulica, I}
abstractNote = {The powdered culture media (RPMI - 1640) were reconstituted either with normal distilled water (150 ppm deuterium) either with deuterium - depleted water (DDW) in various concentrations (30, 60, 90 ppm) and sterilized by filtration with 0.2 {mu}m filters. The cell lines used were NIH (normal mouse fibroblasts), RAG (mouse renal carcinoma) and TS/A (mouse mammary adenocarcinoma). In auxiliary tests, BAIBC mouse splenocytes in direct culture were used, stimulated for growth with concanavalin A or LPS (bacterial lipopolysaccharide). The estimation of the growth was made using the MTT assay or direct counting with trypan blue exclusion. The following results were obtained: Deuterium - depleted water had a stimulating effect on cell growth, the most important stimulating action being from the 90 ppm deuterium-water. The growth curves show, in a first phase, a stimulation of the rapid -growing neoplastic cells, followed by a slower growth of the normal cells. Amiloride 100 mM blocking of the Na{sup +}/K{sup +} membrane pump did not affect the cell growth curves, while the lansoprazole 100 mM blocking of the K{sup +}/H{sup +} ATP-ase brought the growth curves at the level of those with normal water. This might show an eventual involvement of the K{sup +}/H{sup +} antiport in the stimulating effects of the DDW. (authors)}
place = {Romania}
year = {2000}
month = {Jul}
}