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Towards controlled mutagenesis with transposons Ac and Tam3

Abstract

Full text: The discovery of mobile genetic elements in plants has permitted the use of these transposons for insertional mutagenesis. This applies so far only to Zea mays and Antirrhinum majus, because other plant transposable elements have not been characterised so thoroughly at the genetic and the molecular level. To establish whether transposons (Ac from maize and Tam3 from Antirrhinum) remain mobile in heterologous hosts, either in somatic tissue or after meiosis, a phenotypic assay system for transposition was developed. The separation of the two transposition functions will allow controlled mutagenesis of plant genes. Our results indicate that both transposable elements remain active in heterologous hosts. (author)
Authors:
Haring, M; Veken, J; Windrich, R; Kneppers, T; Rommens, C; Nijkamp, H J.J.; Hille, J [1] 
  1. Department of Genetics, Free University, Amsterdam (Netherlands)
Publication Date:
Jan 01, 1990
Product Type:
Conference
Report Number:
INIS-XA-477
Resource Relation:
Conference: 12. EUCARPIA congress on science for plant breeding, Goettingen (Germany), 27 Feb - 3 Mar 1989; Other Information: PBD: Jan 1990; Related Information: In: Mutation breeding newsletter. No. 35, 44 pages.
Subject:
60 APPLIED LIFE SCIENCES; GENETIC ENGINEERING; MAIZE; MEIOSIS; MUTAGENESIS; MUTAGENS; PLANT BREEDING
OSTI ID:
20238378
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 1011-260X; TRN: XA0200703012651
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 15
Announcement Date:

Citation Formats

Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J. Towards controlled mutagenesis with transposons Ac and Tam3. IAEA: N. p., 1990. Web.
Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., & Hille, J. Towards controlled mutagenesis with transposons Ac and Tam3. IAEA.
Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J. 1990. "Towards controlled mutagenesis with transposons Ac and Tam3." IAEA.
@misc{etde_20238378,
title = {Towards controlled mutagenesis with transposons Ac and Tam3}
author = {Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J}
abstractNote = {Full text: The discovery of mobile genetic elements in plants has permitted the use of these transposons for insertional mutagenesis. This applies so far only to Zea mays and Antirrhinum majus, because other plant transposable elements have not been characterised so thoroughly at the genetic and the molecular level. To establish whether transposons (Ac from maize and Tam3 from Antirrhinum) remain mobile in heterologous hosts, either in somatic tissue or after meiosis, a phenotypic assay system for transposition was developed. The separation of the two transposition functions will allow controlled mutagenesis of plant genes. Our results indicate that both transposable elements remain active in heterologous hosts. (author)}
place = {IAEA}
year = {1990}
month = {Jan}
}