Abstract
Full text: The discovery of mobile genetic elements in plants has permitted the use of these transposons for insertional mutagenesis. This applies so far only to Zea mays and Antirrhinum majus, because other plant transposable elements have not been characterised so thoroughly at the genetic and the molecular level. To establish whether transposons (Ac from maize and Tam3 from Antirrhinum) remain mobile in heterologous hosts, either in somatic tissue or after meiosis, a phenotypic assay system for transposition was developed. The separation of the two transposition functions will allow controlled mutagenesis of plant genes. Our results indicate that both transposable elements remain active in heterologous hosts. (author)
Haring, M;
Veken, J;
Windrich, R;
Kneppers, T;
Rommens, C;
Nijkamp, H J.J.;
Hille, J
[1]
- Department of Genetics, Free University, Amsterdam (Netherlands)
Citation Formats
Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J.
Towards controlled mutagenesis with transposons Ac and Tam3.
IAEA: N. p.,
1990.
Web.
Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., & Hille, J.
Towards controlled mutagenesis with transposons Ac and Tam3.
IAEA.
Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J.
1990.
"Towards controlled mutagenesis with transposons Ac and Tam3."
IAEA.
@misc{etde_20238378,
title = {Towards controlled mutagenesis with transposons Ac and Tam3}
author = {Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J}
abstractNote = {Full text: The discovery of mobile genetic elements in plants has permitted the use of these transposons for insertional mutagenesis. This applies so far only to Zea mays and Antirrhinum majus, because other plant transposable elements have not been characterised so thoroughly at the genetic and the molecular level. To establish whether transposons (Ac from maize and Tam3 from Antirrhinum) remain mobile in heterologous hosts, either in somatic tissue or after meiosis, a phenotypic assay system for transposition was developed. The separation of the two transposition functions will allow controlled mutagenesis of plant genes. Our results indicate that both transposable elements remain active in heterologous hosts. (author)}
place = {IAEA}
year = {1990}
month = {Jan}
}
title = {Towards controlled mutagenesis with transposons Ac and Tam3}
author = {Haring, M, Veken, J, Windrich, R, Kneppers, T, Rommens, C, Nijkamp, H J.J., and Hille, J}
abstractNote = {Full text: The discovery of mobile genetic elements in plants has permitted the use of these transposons for insertional mutagenesis. This applies so far only to Zea mays and Antirrhinum majus, because other plant transposable elements have not been characterised so thoroughly at the genetic and the molecular level. To establish whether transposons (Ac from maize and Tam3 from Antirrhinum) remain mobile in heterologous hosts, either in somatic tissue or after meiosis, a phenotypic assay system for transposition was developed. The separation of the two transposition functions will allow controlled mutagenesis of plant genes. Our results indicate that both transposable elements remain active in heterologous hosts. (author)}
place = {IAEA}
year = {1990}
month = {Jan}
}