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Study on antibacterial activity of hydrogel from irradiated silk protein

Abstract

Hydrogels for biomedical application were prepared from solution blends of 3% silk protein and 3%, 10% poly (vinyl alcohol) (PVA) and followed with irradiation. Mixture of hydrogels were gamma irradiated at 10, 20, 30, 40 and 50 kGy under N{sub 2} atmosphere. To clarify anti-bacterial activity of hydrogels, modified of the Agar disk diffusion method and American Association of Textile Chemists and Colorists, AATCC Test Method 90-1977, were carried out. The four kinds of bacteria such as Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis, were used. It was found that a 1:3 volume ratio of 3% silk protein and 3% PVA respectively, at 50 kGy irradiation, is suitable conditions for preparation hydrogels and trend to indicate the highest of an antibacterial activity against E. coli, B. subtilis and S. aureus. However the antibacterial activity of hydrogels against S. epidermidis was not clearly. These results are very useful to expand the application of hydrogel from irradiated silk protein to the medical products. (author)
Authors:
Bunnak, J; Chaisupakitsin, M [1] 
  1. King Mongkut's Institute of Technology Lardkrabang, Bangkok (Thailand)
Publication Date:
Mar 01, 2001
Product Type:
Conference
Report Number:
JAERI-Conf-2001-005
Resource Relation:
Conference: Takasaki symposium on radiation processing of natural polymers, Takasaki, Gunma (Japan), 23-24 Nov 2000; Other Information: 17 refs., 10 figs.; PBD: Mar 2001; Related Information: In: Proceedings of the Takasaki symposium on radiation processing of natural polymers, by Kume, Tamikazu; Maekawa, Yasunari (eds.) [Japan Atomic Energy Research Inst., Takasaki, Gunma (Japan). Takasaki Radiation Chemistry Research Establishment], 288 pages.
Subject:
60 APPLIED LIFE SCIENCES; RADIATION CHEMISTRY; SILKWORM; KERATIN; COBALT 60; GELS; ESCHERICHIA COLI; BACILLUS SUBTILIS; STAPHYLOCOCCUS; GROWTH; INHIBITION
OSTI ID:
20226911
Research Organizations:
Japan Atomic Energy Research Inst., Tokyo (Japan)
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
TRN: JP0150465
Availability:
INIS
Submitting Site:
JPN
Size:
page(s) 117-129
Announcement Date:

Citation Formats

Bunnak, J, and Chaisupakitsin, M. Study on antibacterial activity of hydrogel from irradiated silk protein. Japan: N. p., 2001. Web.
Bunnak, J, & Chaisupakitsin, M. Study on antibacterial activity of hydrogel from irradiated silk protein. Japan.
Bunnak, J, and Chaisupakitsin, M. 2001. "Study on antibacterial activity of hydrogel from irradiated silk protein." Japan.
@misc{etde_20226911,
title = {Study on antibacterial activity of hydrogel from irradiated silk protein}
author = {Bunnak, J, and Chaisupakitsin, M}
abstractNote = {Hydrogels for biomedical application were prepared from solution blends of 3% silk protein and 3%, 10% poly (vinyl alcohol) (PVA) and followed with irradiation. Mixture of hydrogels were gamma irradiated at 10, 20, 30, 40 and 50 kGy under N{sub 2} atmosphere. To clarify anti-bacterial activity of hydrogels, modified of the Agar disk diffusion method and American Association of Textile Chemists and Colorists, AATCC Test Method 90-1977, were carried out. The four kinds of bacteria such as Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis, were used. It was found that a 1:3 volume ratio of 3% silk protein and 3% PVA respectively, at 50 kGy irradiation, is suitable conditions for preparation hydrogels and trend to indicate the highest of an antibacterial activity against E. coli, B. subtilis and S. aureus. However the antibacterial activity of hydrogels against S. epidermidis was not clearly. These results are very useful to expand the application of hydrogel from irradiated silk protein to the medical products. (author)}
place = {Japan}
year = {2001}
month = {Mar}
}