Abstract
This paper describes the development and optimization of methodology for total boron concentration in cell cultures coming from fixation and accumulation of this element by normal and malignant cells. On account of sample mass and low volume resulting from dilution, generally about 1 mL, a procedure for automatic injection of micro volumes was designed, developed and optimized. Iron interference was carefully studied. Linear calibration curves were obtained for 50 to 2500 ng B/mL range. Determination limits were 10 and 20 ng B/mL for B 249.772 nm and 249.677 nm, respectively. Repeatability, expressed as relative standard deviation was better than 5% for a 100 ng B/mL. Recovery of analyte added to real samples ranged between 95 and 103%. The method was applied to studies on F-98 cells (rat glioma) and normal glia in BNCT project frame. (author)
Garavaglia, Ricardo N;
Farias, Silvia S;
Rodriguez, Ruben E;
Servant, Roberto E;
Liberman, Sara;
Pisarev, Mario A;
Batistoni, Daniel A
[1]
- Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Quimica
Citation Formats
Garavaglia, Ricardo N, Farias, Silvia S, Rodriguez, Ruben E, Servant, Roberto E, Liberman, Sara, Pisarev, Mario A, and Batistoni, Daniel A.
Determination of boron by ICP-AES in normal and malignant cells incubated 'in vitro' with fructose {sup 10}BPA; Determinacion de boro por ICP-AES para el estudio de captacion por celulas malignas y normales incubadas 'in vitro' con fructosa-BPA.
Argentina: N. p.,
1999.
Web.
Garavaglia, Ricardo N, Farias, Silvia S, Rodriguez, Ruben E, Servant, Roberto E, Liberman, Sara, Pisarev, Mario A, & Batistoni, Daniel A.
Determination of boron by ICP-AES in normal and malignant cells incubated 'in vitro' with fructose {sup 10}BPA; Determinacion de boro por ICP-AES para el estudio de captacion por celulas malignas y normales incubadas 'in vitro' con fructosa-BPA.
Argentina.
Garavaglia, Ricardo N, Farias, Silvia S, Rodriguez, Ruben E, Servant, Roberto E, Liberman, Sara, Pisarev, Mario A, and Batistoni, Daniel A.
1999.
"Determination of boron by ICP-AES in normal and malignant cells incubated 'in vitro' with fructose {sup 10}BPA; Determinacion de boro por ICP-AES para el estudio de captacion por celulas malignas y normales incubadas 'in vitro' con fructosa-BPA."
Argentina.
@misc{etde_20207128,
title = {Determination of boron by ICP-AES in normal and malignant cells incubated 'in vitro' with fructose {sup 10}BPA; Determinacion de boro por ICP-AES para el estudio de captacion por celulas malignas y normales incubadas 'in vitro' con fructosa-BPA}
author = {Garavaglia, Ricardo N, Farias, Silvia S, Rodriguez, Ruben E, Servant, Roberto E, Liberman, Sara, Pisarev, Mario A, and Batistoni, Daniel A}
abstractNote = {This paper describes the development and optimization of methodology for total boron concentration in cell cultures coming from fixation and accumulation of this element by normal and malignant cells. On account of sample mass and low volume resulting from dilution, generally about 1 mL, a procedure for automatic injection of micro volumes was designed, developed and optimized. Iron interference was carefully studied. Linear calibration curves were obtained for 50 to 2500 ng B/mL range. Determination limits were 10 and 20 ng B/mL for B 249.772 nm and 249.677 nm, respectively. Repeatability, expressed as relative standard deviation was better than 5% for a 100 ng B/mL. Recovery of analyte added to real samples ranged between 95 and 103%. The method was applied to studies on F-98 cells (rat glioma) and normal glia in BNCT project frame. (author)}
place = {Argentina}
year = {1999}
month = {Jul}
}
title = {Determination of boron by ICP-AES in normal and malignant cells incubated 'in vitro' with fructose {sup 10}BPA; Determinacion de boro por ICP-AES para el estudio de captacion por celulas malignas y normales incubadas 'in vitro' con fructosa-BPA}
author = {Garavaglia, Ricardo N, Farias, Silvia S, Rodriguez, Ruben E, Servant, Roberto E, Liberman, Sara, Pisarev, Mario A, and Batistoni, Daniel A}
abstractNote = {This paper describes the development and optimization of methodology for total boron concentration in cell cultures coming from fixation and accumulation of this element by normal and malignant cells. On account of sample mass and low volume resulting from dilution, generally about 1 mL, a procedure for automatic injection of micro volumes was designed, developed and optimized. Iron interference was carefully studied. Linear calibration curves were obtained for 50 to 2500 ng B/mL range. Determination limits were 10 and 20 ng B/mL for B 249.772 nm and 249.677 nm, respectively. Repeatability, expressed as relative standard deviation was better than 5% for a 100 ng B/mL. Recovery of analyte added to real samples ranged between 95 and 103%. The method was applied to studies on F-98 cells (rat glioma) and normal glia in BNCT project frame. (author)}
place = {Argentina}
year = {1999}
month = {Jul}
}