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Putative radioresistant bacterial isolate from sewage water

Abstract

Sewage water was collected from a stagnant body of water in Balara, Quezon City. approximately 150 ml was aseptically transferred into eight Erlenmeyer flasks. Seven flasks were then subjected to different doses of radiation at the {sup 60}Co irradiation facility, PNRI (Philippine Nuclear Research Institute) which are as follows: 0.01 kGy, 0.1 kGy, 0.5 kGy, 1 kGy, 5 kGy, 10 kGy, and 15 kGy. The remaining flask was used as the control. After irradiation, all the different treatments were subjected to colony count at the culture collection laboratory, NSRI. Results showed that the colonies from sewage water treatments irradiated at 0.01 kGy (treatment A), 0.10 kGy (treatment B), and 0.50 kGy (treatment C) exhibited a decreasing trend with colony counts 4.60 x 10{sup 3} CFU/ml, and 1.30 x 10{sup 3} CFU/ml, and 26 CFU/ml, respectively. Contrastingly, at 1 kGy (treatment D), high colony count of 2.95 x 10{sup 3} CFU/ml was observed which is even higher compared to the control (1.02 x 10{sup 3} CFU/ml). Treatment E that was irradiated at 5 kGy manifested low survival rate (25 CFU/ml) indicating the presence of few putative intermediate radioresistant bacteria. Radiation dose treatments higher than 5 kGy (i.e., 10 kGy and 15  More>>
Publication Date:
Jan 29, 2001
Product Type:
Thesis/Dissertation
Report Number:
INIS-PH-015
Reference Number:
EDB-01:089702
Resource Relation:
Other Information: TH: B.S. Chemistry; 26 refs, 3 figs, 1 tab; PBD: 29 Jan 2001
Subject:
07 ISOTOPES AND RADIATION SOURCES; COBALT 60; IRRADIATION; LIQUID WASTES; PHILIPPINES; RADIATION DOSES; SEWAGE; WASTE PROCESSING; WATER; WATER POLLUTION
OSTI ID:
20194801
Research Organizations:
Miriam College High School, Quezon City (Philippines)
Country of Origin:
Philippines
Language:
English
Other Identifying Numbers:
TRN: PH0100007046477
Availability:
Available from INIS in electronic form; Also available from PNRI library
Submitting Site:
INIS
Size:
19 pages
Announcement Date:

Citation Formats

Ang, April, Chua, Patricia, Perez, Kristine, Rey, April, Kristel, Rivor, San Pablo, Czarina, and Santos, Ernestine. Putative radioresistant bacterial isolate from sewage water. Philippines: N. p., 2001. Web.
Ang, April, Chua, Patricia, Perez, Kristine, Rey, April, Kristel, Rivor, San Pablo, Czarina, & Santos, Ernestine. Putative radioresistant bacterial isolate from sewage water. Philippines.
Ang, April, Chua, Patricia, Perez, Kristine, Rey, April, Kristel, Rivor, San Pablo, Czarina, and Santos, Ernestine. 2001. "Putative radioresistant bacterial isolate from sewage water." Philippines.
@misc{etde_20194801,
title = {Putative radioresistant bacterial isolate from sewage water}
author = {Ang, April, Chua, Patricia, Perez, Kristine, Rey, April, Kristel, Rivor, San Pablo, Czarina, and Santos, Ernestine}
abstractNote = {Sewage water was collected from a stagnant body of water in Balara, Quezon City. approximately 150 ml was aseptically transferred into eight Erlenmeyer flasks. Seven flasks were then subjected to different doses of radiation at the {sup 60}Co irradiation facility, PNRI (Philippine Nuclear Research Institute) which are as follows: 0.01 kGy, 0.1 kGy, 0.5 kGy, 1 kGy, 5 kGy, 10 kGy, and 15 kGy. The remaining flask was used as the control. After irradiation, all the different treatments were subjected to colony count at the culture collection laboratory, NSRI. Results showed that the colonies from sewage water treatments irradiated at 0.01 kGy (treatment A), 0.10 kGy (treatment B), and 0.50 kGy (treatment C) exhibited a decreasing trend with colony counts 4.60 x 10{sup 3} CFU/ml, and 1.30 x 10{sup 3} CFU/ml, and 26 CFU/ml, respectively. Contrastingly, at 1 kGy (treatment D), high colony count of 2.95 x 10{sup 3} CFU/ml was observed which is even higher compared to the control (1.02 x 10{sup 3} CFU/ml). Treatment E that was irradiated at 5 kGy manifested low survival rate (25 CFU/ml) indicating the presence of few putative intermediate radioresistant bacteria. Radiation dose treatments higher than 5 kGy (i.e., 10 kGy and 15 kGy) exhibited no bacterial survival. (Author)}
place = {Philippines}
year = {2001}
month = {Jan}
}