Abstract
In vitro cultured shoots of potato, cvs. 'Kufri Jyoti' and 'Kufri Chandramukhi', were irradiated with 20 and 40 Gy gamma rays. Microtubers, obtained from MIV3 shoots multiplied in vitro, were planted in pots. The resulting plants were screened for resistance to late blight, using detached leaf method. In 'Kufri Chandramukhi', 42% plants and in 'Kufri Jyoti' 36% plants, obtained from 40 Gy treatment, showed resistance to late blight. The frequency of resistant plants was lower from 20 Gy treatment. The progenies of putatively resistant plants were grown in field, and inoculated with sporangial inoculum of late blight fungus. The field grown progeny segregated for disease resistance, and approximately 56% plants showed resistance. During the next propagation, the frequency of resistant plants increased to 72%. For developing heat tolerance, microtubers obtained from 20 and 40 Gy treatments and in vitro multiplied M{sub 1}V{sub 3} shoots were cultured at high temperature of 28C. In both varieties, the number of the microtubers per plant was highly reduced and the resulting microtubers had distorted shape but showed better germination (62%), even in early sowing at relatively higher temperature. Of the two radiation doses, the higher dose of 40 Gy gave better results in both
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Gosal, S S;
Das, A;
Gopal, J;
Minocha, J L;
Chopra, H R;
Dhaliwal, H S
[1]
- Biotechnology Centre, Punjab Agricultural University, Ludhiana, Punjab (India)
Citation Formats
Gosal, S S, Das, A, Gopal, J, Minocha, J L, Chopra, H R, and Dhaliwal, H S.
In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato.
IAEA: N. p.,
2001.
Web.
Gosal, S S, Das, A, Gopal, J, Minocha, J L, Chopra, H R, & Dhaliwal, H S.
In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato.
IAEA.
Gosal, S S, Das, A, Gopal, J, Minocha, J L, Chopra, H R, and Dhaliwal, H S.
2001.
"In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato."
IAEA.
@misc{etde_20181058,
title = {In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato}
author = {Gosal, S S, Das, A, Gopal, J, Minocha, J L, Chopra, H R, and Dhaliwal, H S}
abstractNote = {In vitro cultured shoots of potato, cvs. 'Kufri Jyoti' and 'Kufri Chandramukhi', were irradiated with 20 and 40 Gy gamma rays. Microtubers, obtained from MIV3 shoots multiplied in vitro, were planted in pots. The resulting plants were screened for resistance to late blight, using detached leaf method. In 'Kufri Chandramukhi', 42% plants and in 'Kufri Jyoti' 36% plants, obtained from 40 Gy treatment, showed resistance to late blight. The frequency of resistant plants was lower from 20 Gy treatment. The progenies of putatively resistant plants were grown in field, and inoculated with sporangial inoculum of late blight fungus. The field grown progeny segregated for disease resistance, and approximately 56% plants showed resistance. During the next propagation, the frequency of resistant plants increased to 72%. For developing heat tolerance, microtubers obtained from 20 and 40 Gy treatments and in vitro multiplied M{sub 1}V{sub 3} shoots were cultured at high temperature of 28C. In both varieties, the number of the microtubers per plant was highly reduced and the resulting microtubers had distorted shape but showed better germination (62%), even in early sowing at relatively higher temperature. Of the two radiation doses, the higher dose of 40 Gy gave better results in both the varieties. Heat tolerance was also assessed from chlorophyll persistence. The progenies from putative heat-tolerant plants were tested in field by planting at higher temperature in two subsequent generations. The heat tolerant plants segregated in each generation, but the frequency of heat-tolerant plants increased. (author)}
place = {IAEA}
year = {2001}
month = {Jun}
}
title = {In vitro induction of variability through radiation for late blight resistance and heat tolerance in potato}
author = {Gosal, S S, Das, A, Gopal, J, Minocha, J L, Chopra, H R, and Dhaliwal, H S}
abstractNote = {In vitro cultured shoots of potato, cvs. 'Kufri Jyoti' and 'Kufri Chandramukhi', were irradiated with 20 and 40 Gy gamma rays. Microtubers, obtained from MIV3 shoots multiplied in vitro, were planted in pots. The resulting plants were screened for resistance to late blight, using detached leaf method. In 'Kufri Chandramukhi', 42% plants and in 'Kufri Jyoti' 36% plants, obtained from 40 Gy treatment, showed resistance to late blight. The frequency of resistant plants was lower from 20 Gy treatment. The progenies of putatively resistant plants were grown in field, and inoculated with sporangial inoculum of late blight fungus. The field grown progeny segregated for disease resistance, and approximately 56% plants showed resistance. During the next propagation, the frequency of resistant plants increased to 72%. For developing heat tolerance, microtubers obtained from 20 and 40 Gy treatments and in vitro multiplied M{sub 1}V{sub 3} shoots were cultured at high temperature of 28C. In both varieties, the number of the microtubers per plant was highly reduced and the resulting microtubers had distorted shape but showed better germination (62%), even in early sowing at relatively higher temperature. Of the two radiation doses, the higher dose of 40 Gy gave better results in both the varieties. Heat tolerance was also assessed from chlorophyll persistence. The progenies from putative heat-tolerant plants were tested in field by planting at higher temperature in two subsequent generations. The heat tolerant plants segregated in each generation, but the frequency of heat-tolerant plants increased. (author)}
place = {IAEA}
year = {2001}
month = {Jun}
}