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''In vitro'' mutation breeding methodology for Fusarium wilt resistance in banana

Abstract

Full text: Besides ''in vivo'' methods, the Radiation Genetics Section of CENA/USP is also using ''in vitro'' methods for mutation breeding to obtain resistance to Panama disease caused by Fusarium oxysporum f.sp. cubense in the banana cultivar ''Maca''. A protocol has been established for the ''in vitro'' development of shoot tips, obtained from plants in the field or already cultivated under {sup i}n vitro'' conditions. For both cases, only one culture medium was used during all steps of ''in vitro'' cultivation. New buds were formed and these buds grew and developed to form roots. The medium was composed of macro and micro nutrients, with added Morel vitamines, BAP (5 mg/l), saccharose (30 g/l) and agar (6.5 g/l), at pH 5.7. Cultures were allowed to grow in a controlled environment at 27 deg. C and 16 h illumination. Shoot tips which originated from ''in vitro'' plantlets, were cut longitudinally down the middle. This was done to avoid a tendency of regeneration of the original tissue instead of the formation of new lateral buds. To resolve the chimerism resulting when mutagenic treatment is applied to shoot tips, there is a need of vegetative propagation of new lateral buds. Selection can then be  More>>
Authors:
Tulmann Neto, A; Domingues, E T; Mendes, B M.J.; Ando, A [1] 
  1. Centre for Nuclear Energy in Agriculture (CENA), Piracicaba, SP. (Brazil)
Publication Date:
Jul 01, 1990
Product Type:
Technical Report
Report Number:
INIS-XA-405
Reference Number:
EDB-01:068121
Resource Relation:
Other Information: 1 ref; PBD: Jul 1990; Related Information: In: Mutation breeding newsletter. No. 36, 32 pages.
Subject:
60 APPLIED LIFE SCIENCES; BANANA PLANTS; BANANAS; DISEASE RESISTANCE; FUSARIUM; GAMMA RADIATION; IN VIVO; IRRADIATION; PLANT BREEDING; PLANT DISEASES; PRODUCTIVITY; RADIATION DOSES; RADIATION INDUCED MUTANTS
OSTI ID:
20180122
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 1011-260X; TRN: XA0101392036241
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 12-13
Announcement Date:
Aug 16, 2001

Citation Formats

Tulmann Neto, A, Domingues, E T, Mendes, B M.J., and Ando, A. ''In vitro'' mutation breeding methodology for Fusarium wilt resistance in banana. IAEA: N. p., 1990. Web.
Tulmann Neto, A, Domingues, E T, Mendes, B M.J., & Ando, A. ''In vitro'' mutation breeding methodology for Fusarium wilt resistance in banana. IAEA.
Tulmann Neto, A, Domingues, E T, Mendes, B M.J., and Ando, A. 1990. "''In vitro'' mutation breeding methodology for Fusarium wilt resistance in banana." IAEA.
@misc{etde_20180122,
title = {''In vitro'' mutation breeding methodology for Fusarium wilt resistance in banana}
author = {Tulmann Neto, A, Domingues, E T, Mendes, B M.J., and Ando, A}
abstractNote = {Full text: Besides ''in vivo'' methods, the Radiation Genetics Section of CENA/USP is also using ''in vitro'' methods for mutation breeding to obtain resistance to Panama disease caused by Fusarium oxysporum f.sp. cubense in the banana cultivar ''Maca''. A protocol has been established for the ''in vitro'' development of shoot tips, obtained from plants in the field or already cultivated under {sup i}n vitro'' conditions. For both cases, only one culture medium was used during all steps of ''in vitro'' cultivation. New buds were formed and these buds grew and developed to form roots. The medium was composed of macro and micro nutrients, with added Morel vitamines, BAP (5 mg/l), saccharose (30 g/l) and agar (6.5 g/l), at pH 5.7. Cultures were allowed to grow in a controlled environment at 27 deg. C and 16 h illumination. Shoot tips which originated from ''in vitro'' plantlets, were cut longitudinally down the middle. This was done to avoid a tendency of regeneration of the original tissue instead of the formation of new lateral buds. To resolve the chimerism resulting when mutagenic treatment is applied to shoot tips, there is a need of vegetative propagation of new lateral buds. Selection can then be done at M{sub 1}V{sub 4} generation. Once the protocol was established, the gamma ray sensitivity was determined. The dose that produced a 50% decrease in the number of new lateral buds was around 40 Gy and this dose will be utilised. The methodology was completed by soil inoculation with Fusarium of young plants 15 cm in height, obtained from ''in vitro'' cultures. After 3 weeks all inoculated plants showed symptoms of wilt, demonstrating the possibility of screening. The method is now being utilised on a large scale in an attempt to induce a resistant mutant. (author)}
place = {IAEA}
year = {1990}
month = {Jul}
}