Abstract
Oysters (Crassostrea virginica) collected on the Cuban coast near Havana were examined for contamination with Vibrio cholerae and other potentially pathogenic Vibrio species. The strains thus isolated were characterized and identified to species following standard methods, and their radiation resistance (D{sub 10}) was determined in pure culture. The Vibrio species most often isolated were V. cholerae, V. parahaemolyticus and V. Alginolyticus. Representative cultures from each species were later used to inoculate shucked oysters to determine the optimal radiation dose that would ensure elimination of 10{sup 8} colony forming units (CFU)/g. The highest proportion of isolates were identified as Vibrio parahaemolyticus and V. algynoliticus. Non-O1 strains of Vibrio cholerae were isolated from 50% of samples, but no V. cholerae O1 was identified. D{sub 10} values calculated for the various strains were low in relation to those in the literature. The radiation dose for decontaminating heavily inoculated (10{sup 8} CFU/g) oysters was 1.2 kGy. (author)
Cisneros Despaigne, E;
Leyva Castillo, V;
Martinez, L L;
Lara Ortiz, C;
[1]
Castillo Rodriguez, E
[2]
- Instituto de Nutricion e Higiene de los Alimentos (Cuba)
- Centro Nacional de Salud Animal (Cuba)
Citation Formats
Cisneros Despaigne, E, Leyva Castillo, V, Martinez, L L, Lara Ortiz, C, and Castillo Rodriguez, E.
Decontamination of Cuban oysters using irradiation.
IAEA: N. p.,
2001.
Web.
Cisneros Despaigne, E, Leyva Castillo, V, Martinez, L L, Lara Ortiz, C, & Castillo Rodriguez, E.
Decontamination of Cuban oysters using irradiation.
IAEA.
Cisneros Despaigne, E, Leyva Castillo, V, Martinez, L L, Lara Ortiz, C, and Castillo Rodriguez, E.
2001.
"Decontamination of Cuban oysters using irradiation."
IAEA.
@misc{etde_20161856,
title = {Decontamination of Cuban oysters using irradiation}
author = {Cisneros Despaigne, E, Leyva Castillo, V, Martinez, L L, Lara Ortiz, C, and Castillo Rodriguez, E}
abstractNote = {Oysters (Crassostrea virginica) collected on the Cuban coast near Havana were examined for contamination with Vibrio cholerae and other potentially pathogenic Vibrio species. The strains thus isolated were characterized and identified to species following standard methods, and their radiation resistance (D{sub 10}) was determined in pure culture. The Vibrio species most often isolated were V. cholerae, V. parahaemolyticus and V. Alginolyticus. Representative cultures from each species were later used to inoculate shucked oysters to determine the optimal radiation dose that would ensure elimination of 10{sup 8} colony forming units (CFU)/g. The highest proportion of isolates were identified as Vibrio parahaemolyticus and V. algynoliticus. Non-O1 strains of Vibrio cholerae were isolated from 50% of samples, but no V. cholerae O1 was identified. D{sub 10} values calculated for the various strains were low in relation to those in the literature. The radiation dose for decontaminating heavily inoculated (10{sup 8} CFU/g) oysters was 1.2 kGy. (author)}
place = {IAEA}
year = {2001}
month = {Apr}
}
title = {Decontamination of Cuban oysters using irradiation}
author = {Cisneros Despaigne, E, Leyva Castillo, V, Martinez, L L, Lara Ortiz, C, and Castillo Rodriguez, E}
abstractNote = {Oysters (Crassostrea virginica) collected on the Cuban coast near Havana were examined for contamination with Vibrio cholerae and other potentially pathogenic Vibrio species. The strains thus isolated were characterized and identified to species following standard methods, and their radiation resistance (D{sub 10}) was determined in pure culture. The Vibrio species most often isolated were V. cholerae, V. parahaemolyticus and V. Alginolyticus. Representative cultures from each species were later used to inoculate shucked oysters to determine the optimal radiation dose that would ensure elimination of 10{sup 8} colony forming units (CFU)/g. The highest proportion of isolates were identified as Vibrio parahaemolyticus and V. algynoliticus. Non-O1 strains of Vibrio cholerae were isolated from 50% of samples, but no V. cholerae O1 was identified. D{sub 10} values calculated for the various strains were low in relation to those in the literature. The radiation dose for decontaminating heavily inoculated (10{sup 8} CFU/g) oysters was 1.2 kGy. (author)}
place = {IAEA}
year = {2001}
month = {Apr}
}