Abstract
The present investigation included the isolation and identification of rennin like enzyme fungal producer from different egyptian food and soil samples. Only six out of three hundred and ninety five fungal cultures isolated from cheese, yogurt and soil samples collected from different localities of egypt were found to be positive for the production of clotting enzymes. The six positive isolates were identified as: paecilomyces variotii, Mucor circinelloides, aspergillus niger, aspergillus flavus, Emericella variecolor and Cochliobolus spicifier. All isolates were found to be negative for mycotoxins but Mucor Lamprosporus isolate obtained from the CBS, Baarn, netherlands was the best in the production of the crude enzyme. The highest growth and production of the crude enzyme were obtained from wheat bran medium after an incubation period for 7-9 days at 25 degree C and Ph 5.0. wheat bran medium could also be used several times to produce the enzyme. Mucor lamprospours spores were found to be sensitive to gamma rays since 2.5 kGy completely inactivated the germination of the spore sand D{sub 10} value of the spores was calculated to be only 0.36 kGy and very low doses of gamma irradiation did not affect the ability of the isolates to produce the
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Moussa, L A;
El-Fouly, M Z;
El-Kabbany, H;
[1]
Kamel, Z M;
Moubasher, M H
[2]
- National Center for Radiation Research and Technology, P.O.Box 29, Cairo (Egypt)
- Botany Department, Faculty od Science, Cairo University, Giza (Egypt)
Citation Formats
Moussa, L A, El-Fouly, M Z, El-Kabbany, H, Kamel, Z M, and Moubasher, M H.
Effect of environmental conditions and Gamma Irradiation on the productivity of rennin-like enzyme from Mucor Lamprosporus.
Egypt: N. p.,
2000.
Web.
Moussa, L A, El-Fouly, M Z, El-Kabbany, H, Kamel, Z M, & Moubasher, M H.
Effect of environmental conditions and Gamma Irradiation on the productivity of rennin-like enzyme from Mucor Lamprosporus.
Egypt.
Moussa, L A, El-Fouly, M Z, El-Kabbany, H, Kamel, Z M, and Moubasher, M H.
2000.
"Effect of environmental conditions and Gamma Irradiation on the productivity of rennin-like enzyme from Mucor Lamprosporus."
Egypt.
@misc{etde_20153283,
title = {Effect of environmental conditions and Gamma Irradiation on the productivity of rennin-like enzyme from Mucor Lamprosporus}
author = {Moussa, L A, El-Fouly, M Z, El-Kabbany, H, Kamel, Z M, and Moubasher, M H}
abstractNote = {The present investigation included the isolation and identification of rennin like enzyme fungal producer from different egyptian food and soil samples. Only six out of three hundred and ninety five fungal cultures isolated from cheese, yogurt and soil samples collected from different localities of egypt were found to be positive for the production of clotting enzymes. The six positive isolates were identified as: paecilomyces variotii, Mucor circinelloides, aspergillus niger, aspergillus flavus, Emericella variecolor and Cochliobolus spicifier. All isolates were found to be negative for mycotoxins but Mucor Lamprosporus isolate obtained from the CBS, Baarn, netherlands was the best in the production of the crude enzyme. The highest growth and production of the crude enzyme were obtained from wheat bran medium after an incubation period for 7-9 days at 25 degree C and Ph 5.0. wheat bran medium could also be used several times to produce the enzyme. Mucor lamprospours spores were found to be sensitive to gamma rays since 2.5 kGy completely inactivated the germination of the spore sand D{sub 10} value of the spores was calculated to be only 0.36 kGy and very low doses of gamma irradiation did not affect the ability of the isolates to produce the milk clotting enzyme till 100 Gy.}
place = {Egypt}
year = {2000}
month = {Jul}
}
title = {Effect of environmental conditions and Gamma Irradiation on the productivity of rennin-like enzyme from Mucor Lamprosporus}
author = {Moussa, L A, El-Fouly, M Z, El-Kabbany, H, Kamel, Z M, and Moubasher, M H}
abstractNote = {The present investigation included the isolation and identification of rennin like enzyme fungal producer from different egyptian food and soil samples. Only six out of three hundred and ninety five fungal cultures isolated from cheese, yogurt and soil samples collected from different localities of egypt were found to be positive for the production of clotting enzymes. The six positive isolates were identified as: paecilomyces variotii, Mucor circinelloides, aspergillus niger, aspergillus flavus, Emericella variecolor and Cochliobolus spicifier. All isolates were found to be negative for mycotoxins but Mucor Lamprosporus isolate obtained from the CBS, Baarn, netherlands was the best in the production of the crude enzyme. The highest growth and production of the crude enzyme were obtained from wheat bran medium after an incubation period for 7-9 days at 25 degree C and Ph 5.0. wheat bran medium could also be used several times to produce the enzyme. Mucor lamprospours spores were found to be sensitive to gamma rays since 2.5 kGy completely inactivated the germination of the spore sand D{sub 10} value of the spores was calculated to be only 0.36 kGy and very low doses of gamma irradiation did not affect the ability of the isolates to produce the milk clotting enzyme till 100 Gy.}
place = {Egypt}
year = {2000}
month = {Jul}
}