Abstract
The importance that has to confirm the presence or absence of the chromosome Philadelphia in the diagnostic and follow up of the patient affected with chronic myeloid leukemia and other leukemia. It is considered necessary to implement the molecular diagnostic in Costa Rica. They studied 32 patient affected by Chronic Myeloid Leukemia, 7 by other Myeloproliferative Chronic Disorders and 2 by Myelodysplastic Syndrome. It utilized the sound Trans probe-1 (Oncogene Science, Inc), which was marked with radioactivity ({sup 32}P) or chemiluminescence (digoxigenin). Of the 32 cases affected by L mc, in 28 it was possible to carry out the molecular analysis detecting the characteristic translocation of the chromosome Philadelphia among the Mbcr/c-ABL genes in 21 (75%) of the patients, in 7 (25%) the rearrangement was not found. In seven of the nine affected by other sufferings it was possible to obtain results, 3 that turned out to be positive for the rearrangement among Mbcr/c-ABL and 4 normal. In all the cases, they obtained results marking the sound with radioactivity. However, they tested the marks with digoxigenin in seven of the patients, as an methodological alternative for the laboratories that lacks the requirements to work with radiation. The results obtained were
More>>
Campos Rudin, M;
[1]
Cuenca Berger, P;
[1]
Gutierrez Espeleta, G;
[1]
Jimenez Cruz, G;
[2]
Montero Umana, C;
[2]
Vazquez Castillo, L;
[3]
Ramon Ortiz, M
[3]
- Instituto de Investigaciones en Salud (INISA), Costa Rica (Costa Rica) and Escuela de Biologia
- Hospital Mexico, Costa Rica (Costa Rica). Seccion Hematologia
- Hospital San Juan de Dios, Costa Rica (Costa Rica). Seccion de Hematologia
Citation Formats
Campos Rudin, M, Cuenca Berger, P, Gutierrez Espeleta, G, Jimenez Cruz, G, Montero Umana, C, Vazquez Castillo, L, and Ramon Ortiz, M.
Molecular diagnostic of the philadelphia chromosome; Diagnostico molecular del cromosoma filadelfia.
Costa Rica: N. p.,
1998.
Web.
Campos Rudin, M, Cuenca Berger, P, Gutierrez Espeleta, G, Jimenez Cruz, G, Montero Umana, C, Vazquez Castillo, L, & Ramon Ortiz, M.
Molecular diagnostic of the philadelphia chromosome; Diagnostico molecular del cromosoma filadelfia.
Costa Rica.
Campos Rudin, M, Cuenca Berger, P, Gutierrez Espeleta, G, Jimenez Cruz, G, Montero Umana, C, Vazquez Castillo, L, and Ramon Ortiz, M.
1998.
"Molecular diagnostic of the philadelphia chromosome; Diagnostico molecular del cromosoma filadelfia."
Costa Rica.
@misc{etde_20134322,
title = {Molecular diagnostic of the philadelphia chromosome; Diagnostico molecular del cromosoma filadelfia}
author = {Campos Rudin, M, Cuenca Berger, P, Gutierrez Espeleta, G, Jimenez Cruz, G, Montero Umana, C, Vazquez Castillo, L, and Ramon Ortiz, M}
abstractNote = {The importance that has to confirm the presence or absence of the chromosome Philadelphia in the diagnostic and follow up of the patient affected with chronic myeloid leukemia and other leukemia. It is considered necessary to implement the molecular diagnostic in Costa Rica. They studied 32 patient affected by Chronic Myeloid Leukemia, 7 by other Myeloproliferative Chronic Disorders and 2 by Myelodysplastic Syndrome. It utilized the sound Trans probe-1 (Oncogene Science, Inc), which was marked with radioactivity ({sup 32}P) or chemiluminescence (digoxigenin). Of the 32 cases affected by L mc, in 28 it was possible to carry out the molecular analysis detecting the characteristic translocation of the chromosome Philadelphia among the Mbcr/c-ABL genes in 21 (75%) of the patients, in 7 (25%) the rearrangement was not found. In seven of the nine affected by other sufferings it was possible to obtain results, 3 that turned out to be positive for the rearrangement among Mbcr/c-ABL and 4 normal. In all the cases, they obtained results marking the sound with radioactivity. However, they tested the marks with digoxigenin in seven of the patients, as an methodological alternative for the laboratories that lacks the requirements to work with radiation. The results obtained were identical. (S. Grainger) [Spanish] La relevancia que tiene confirmar la presencia o ausencia del cromosoma Filadelfia en el diagnostico y seguimiento de los pacientes afectados con leucemia mieloide cronica y otras leucemias, se considero necesario implementar el diagnostico molecular en Costa Rica. Se estudiaron 32 pacientes afectados por Leucemia Mieloide Cronica, 7 por otros Desordenes Mieloproleferativos Cronicos y 2 por Sindromes Mielodisplasicos. Se utilizo la sonda Transprobe-1 (Oncogene Science, Inc), la cual fue marcada con radioactividad ({sup 32}P) o quimioluminiscencia (digoxigenina). De los 32 casos afectados por LMC, en 28 se logro realizar el analisis molecular detectandose la translocacion caracteristica del cromosoma Filadelfia entre los genes Mbcr/c-ABL en 21 (75%) de los pacientes, en 7 (25%) no se encontro el rearreglo. En siete de los nueve afectados por otros padecimientos fue posible obtener resultados, 3 que resultaron ser positivos para el rearreglo entre Mbcr/c-ABL y 4 normales. En todos los casos se obtuvo resultados marcando la sonda con radioactividad, sin embargo se probo el marcaje con digoxigenina en siete de los pacientes, como una alternativa metodologica para los laboratorios que carecen de los requerimientos para trabajar con radiacion. Los resultados obtenidos fueron identicos. (author)}
journal = []
issue = {3}
volume = {40}
journal type = {AC}
place = {Costa Rica}
year = {1998}
month = {Jul}
}
title = {Molecular diagnostic of the philadelphia chromosome; Diagnostico molecular del cromosoma filadelfia}
author = {Campos Rudin, M, Cuenca Berger, P, Gutierrez Espeleta, G, Jimenez Cruz, G, Montero Umana, C, Vazquez Castillo, L, and Ramon Ortiz, M}
abstractNote = {The importance that has to confirm the presence or absence of the chromosome Philadelphia in the diagnostic and follow up of the patient affected with chronic myeloid leukemia and other leukemia. It is considered necessary to implement the molecular diagnostic in Costa Rica. They studied 32 patient affected by Chronic Myeloid Leukemia, 7 by other Myeloproliferative Chronic Disorders and 2 by Myelodysplastic Syndrome. It utilized the sound Trans probe-1 (Oncogene Science, Inc), which was marked with radioactivity ({sup 32}P) or chemiluminescence (digoxigenin). Of the 32 cases affected by L mc, in 28 it was possible to carry out the molecular analysis detecting the characteristic translocation of the chromosome Philadelphia among the Mbcr/c-ABL genes in 21 (75%) of the patients, in 7 (25%) the rearrangement was not found. In seven of the nine affected by other sufferings it was possible to obtain results, 3 that turned out to be positive for the rearrangement among Mbcr/c-ABL and 4 normal. In all the cases, they obtained results marking the sound with radioactivity. However, they tested the marks with digoxigenin in seven of the patients, as an methodological alternative for the laboratories that lacks the requirements to work with radiation. The results obtained were identical. (S. Grainger) [Spanish] La relevancia que tiene confirmar la presencia o ausencia del cromosoma Filadelfia en el diagnostico y seguimiento de los pacientes afectados con leucemia mieloide cronica y otras leucemias, se considero necesario implementar el diagnostico molecular en Costa Rica. Se estudiaron 32 pacientes afectados por Leucemia Mieloide Cronica, 7 por otros Desordenes Mieloproleferativos Cronicos y 2 por Sindromes Mielodisplasicos. Se utilizo la sonda Transprobe-1 (Oncogene Science, Inc), la cual fue marcada con radioactividad ({sup 32}P) o quimioluminiscencia (digoxigenina). De los 32 casos afectados por LMC, en 28 se logro realizar el analisis molecular detectandose la translocacion caracteristica del cromosoma Filadelfia entre los genes Mbcr/c-ABL en 21 (75%) de los pacientes, en 7 (25%) no se encontro el rearreglo. En siete de los nueve afectados por otros padecimientos fue posible obtener resultados, 3 que resultaron ser positivos para el rearreglo entre Mbcr/c-ABL y 4 normales. En todos los casos se obtuvo resultados marcando la sonda con radioactividad, sin embargo se probo el marcaje con digoxigenina en siete de los pacientes, como una alternativa metodologica para los laboratorios que carecen de los requerimientos para trabajar con radiacion. Los resultados obtenidos fueron identicos. (author)}
journal = []
issue = {3}
volume = {40}
journal type = {AC}
place = {Costa Rica}
year = {1998}
month = {Jul}
}