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Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine

Journal Article:

Abstract

Glutamate decarboxylase (GAD) activity was studied rat brain parts in a pilocarpine model of epileptic seizures. An increased enzyme activity was found in hippocampus a cerebellum during the acute phase of seizures, while the cortex and cerebellum showed increased GAD activity in the chronic phase of the process. Systematic administration of pilocarpine to rats induces status epilepticus. The aim of this research was to find out if seizures induced by pilocarpine are connected changes in glutamate decarboxylase activity, the enzyme that catalyzes synthesis of inhibitory neurotransmitter GABA. GAD was assayed by means of radiometric method using {sup 14}C-carboxyl-labelled glutamate and measurement of {sup 14}CO{sub 2} radioactivity. Obtained results suggest that pilocarpine seizures are connected with changes of GAD activity in individual parts of rat brain. (authors)
Authors:
Netopilova, M; Drsata, J; [1]  Haugvicova, R; Kubova, H; Mares, P [2] 
  1. Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, 50005 Hradec Kralove (Czech Republic)
  2. Institute of Physiology, Czech Academy of Sciences, 14220 Prague (Czech Republic)
Publication Date:
Jul 01, 1998
Product Type:
Journal Article
Reference Number:
EDB-01:004479
Resource Relation:
Journal Name: Chemicke Zvesti; Journal Volume: 52; Journal Issue: Focus Issue; Other Information: 1 tabs., 2 refs.; Proceedings of the sixteenth Biochemical Congress, October 12-15, 1998, Stara Lesna, Slovakia; Previous name of the journal Chemicke Zvesti was Chemicke Zvesti (used in present time, too); PBD: 1998
Subject:
60 APPLIED LIFE SCIENCES; BIOCHEMICAL REACTION KINETICS; BIOCHEMISTRY; BIOSYNTHESIS; BRAIN; CARBON 14 COMPOUNDS; CARBON DIOXIDE; DECARBOXYLASES; EPILEPSY; EXPERIMENTAL DATA; GLUTAMIC ACID; LABELLED COMPOUNDS; LABELLED POOL TECHNIQUES; LABELLING; METABOLISM; MOLECULAR BIOLOGY; PILOCARPINE; RADIOENZYMATIC ASSAY; RADIONUCLIDE KINETICS; RATS
OSTI ID:
20122016
Country of Origin:
Slovakia
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0366-6352; CHZVAN; Other: Grant GACR--309/97/0552; TRN: SK00K0284062188
Availability:
Available via Internet at URL http://www.savba.sk/sav/inst/chem/chempap.html;INIS
Submitting Site:
INIS
Size:
page(s) 455
Announcement Date:

Journal Article:

Citation Formats

Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P. Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine. Slovakia: N. p., 1998. Web.
Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, & Mares, P. Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine. Slovakia.
Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P. 1998. "Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine." Slovakia.
@misc{etde_20122016,
title = {Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine}
author = {Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P}
abstractNote = {Glutamate decarboxylase (GAD) activity was studied rat brain parts in a pilocarpine model of epileptic seizures. An increased enzyme activity was found in hippocampus a cerebellum during the acute phase of seizures, while the cortex and cerebellum showed increased GAD activity in the chronic phase of the process. Systematic administration of pilocarpine to rats induces status epilepticus. The aim of this research was to find out if seizures induced by pilocarpine are connected changes in glutamate decarboxylase activity, the enzyme that catalyzes synthesis of inhibitory neurotransmitter GABA. GAD was assayed by means of radiometric method using {sup 14}C-carboxyl-labelled glutamate and measurement of {sup 14}CO{sub 2} radioactivity. Obtained results suggest that pilocarpine seizures are connected with changes of GAD activity in individual parts of rat brain. (authors)}
journal = {Chemicke Zvesti}
issue = {Focus Issue}
volume = {52}
journal type = {AC}
place = {Slovakia}
year = {1998}
month = {Jul}
}