Abstract
Glutamate decarboxylase (GAD) activity was studied rat brain parts in a pilocarpine model of epileptic seizures. An increased enzyme activity was found in hippocampus a cerebellum during the acute phase of seizures, while the cortex and cerebellum showed increased GAD activity in the chronic phase of the process. Systematic administration of pilocarpine to rats induces status epilepticus. The aim of this research was to find out if seizures induced by pilocarpine are connected changes in glutamate decarboxylase activity, the enzyme that catalyzes synthesis of inhibitory neurotransmitter GABA. GAD was assayed by means of radiometric method using {sup 14}C-carboxyl-labelled glutamate and measurement of {sup 14}CO{sub 2} radioactivity. Obtained results suggest that pilocarpine seizures are connected with changes of GAD activity in individual parts of rat brain. (authors)
Netopilova, M;
Drsata, J;
[1]
Haugvicova, R;
Kubova, H;
Mares, P
[2]
- Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, 50005 Hradec Kralove (Czech Republic)
- Institute of Physiology, Czech Academy of Sciences, 14220 Prague (Czech Republic)
Citation Formats
Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P.
Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine.
Slovakia: N. p.,
1998.
Web.
Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, & Mares, P.
Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine.
Slovakia.
Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P.
1998.
"Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine."
Slovakia.
@misc{etde_20122016,
title = {Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine}
author = {Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P}
abstractNote = {Glutamate decarboxylase (GAD) activity was studied rat brain parts in a pilocarpine model of epileptic seizures. An increased enzyme activity was found in hippocampus a cerebellum during the acute phase of seizures, while the cortex and cerebellum showed increased GAD activity in the chronic phase of the process. Systematic administration of pilocarpine to rats induces status epilepticus. The aim of this research was to find out if seizures induced by pilocarpine are connected changes in glutamate decarboxylase activity, the enzyme that catalyzes synthesis of inhibitory neurotransmitter GABA. GAD was assayed by means of radiometric method using {sup 14}C-carboxyl-labelled glutamate and measurement of {sup 14}CO{sub 2} radioactivity. Obtained results suggest that pilocarpine seizures are connected with changes of GAD activity in individual parts of rat brain. (authors)}
journal = []
issue = {Focus Issue}
volume = {52}
journal type = {AC}
place = {Slovakia}
year = {1998}
month = {Jul}
}
title = {Glutamate decarboxylase activity in rat brain during experimental epileptic seizures induced by pilocarpine}
author = {Netopilova, M, Drsata, J, Haugvicova, R, Kubova, H, and Mares, P}
abstractNote = {Glutamate decarboxylase (GAD) activity was studied rat brain parts in a pilocarpine model of epileptic seizures. An increased enzyme activity was found in hippocampus a cerebellum during the acute phase of seizures, while the cortex and cerebellum showed increased GAD activity in the chronic phase of the process. Systematic administration of pilocarpine to rats induces status epilepticus. The aim of this research was to find out if seizures induced by pilocarpine are connected changes in glutamate decarboxylase activity, the enzyme that catalyzes synthesis of inhibitory neurotransmitter GABA. GAD was assayed by means of radiometric method using {sup 14}C-carboxyl-labelled glutamate and measurement of {sup 14}CO{sub 2} radioactivity. Obtained results suggest that pilocarpine seizures are connected with changes of GAD activity in individual parts of rat brain. (authors)}
journal = []
issue = {Focus Issue}
volume = {52}
journal type = {AC}
place = {Slovakia}
year = {1998}
month = {Jul}
}