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Development of enhanced radioprotectors

Abstract

Biological model systems such as TSH mutations, animals and their reproductive organs, and cultured cell lines has been set up for screening radioprotective or radiosensitizing activities of natural products. About 100 natural plants were in vivo and in vitro tested. Among them, Salvia miltiorrhiza, Areca catechu, Cornus officinalis, and Portulaca oleracea showed 20% of radioprotective activities. On the other hand, Acanthopanax sessiliflous, Melia japonica, and Agastache rugosa revealed radiosensitizing activities. Experimental studies on the farnesyltransferase inhibitors showed the results that some of the inhibits lead to higher levels of cell death after {gamma} -irradiation and increased radiosensitivity in H-ras-transformed NIH3T3 cells and MCF-10A human tumor cells. Molecular biochemical studies on the mechanism of radioprotection was carried out using cultured cells. The treatment of DTT increased cell survival after gamma-irradiation, decreased in the frequencies of micronucleus, and reduction in DNA fragmentation and apoptotic cells. Induction of apoptosis after irradiation was revealed by the changes in the relative cell death, increase in the relative amount of apoptotic cells, and the induction of DNA fragmentation. Samples shown excellent radioprotective or radiosensitizing activities will be subjected to purification to develop radioprotectants and radiosensitizers applicable to human beings. The result of mechanism studies will be  More>>
Authors:
Kim, Jin Kyu; Park, K B; Lee, Y I [1] 
  1. and others
Publication Date:
Apr 01, 1999
Product Type:
Technical Report
Report Number:
KAERI/RR-1903/98
Reference Number:
EDB-00:107354
Resource Relation:
Other Information: PBD: Apr 1999
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL MODELS; CELL CULTURES; ENZYME INHIBITORS; INDICATORS; RADIATION INJURIES; RADIATION PROTECTION; SENSITIZERS
OSTI ID:
20048157
Research Organizations:
Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)
Country of Origin:
Korea, Republic of
Language:
Korean
Other Identifying Numbers:
TRN: KR0000032011709
Availability:
Available from INIS in electronic form
Submitting Site:
KRN
Size:
140 pages
Announcement Date:
Dec 18, 2000

Citation Formats

Kim, Jin Kyu, Park, K B, and Lee, Y I. Development of enhanced radioprotectors. Korea, Republic of: N. p., 1999. Web.
Kim, Jin Kyu, Park, K B, & Lee, Y I. Development of enhanced radioprotectors. Korea, Republic of.
Kim, Jin Kyu, Park, K B, and Lee, Y I. 1999. "Development of enhanced radioprotectors." Korea, Republic of.
@misc{etde_20048157,
title = {Development of enhanced radioprotectors}
author = {Kim, Jin Kyu, Park, K B, and Lee, Y I}
abstractNote = {Biological model systems such as TSH mutations, animals and their reproductive organs, and cultured cell lines has been set up for screening radioprotective or radiosensitizing activities of natural products. About 100 natural plants were in vivo and in vitro tested. Among them, Salvia miltiorrhiza, Areca catechu, Cornus officinalis, and Portulaca oleracea showed 20% of radioprotective activities. On the other hand, Acanthopanax sessiliflous, Melia japonica, and Agastache rugosa revealed radiosensitizing activities. Experimental studies on the farnesyltransferase inhibitors showed the results that some of the inhibits lead to higher levels of cell death after {gamma} -irradiation and increased radiosensitivity in H-ras-transformed NIH3T3 cells and MCF-10A human tumor cells. Molecular biochemical studies on the mechanism of radioprotection was carried out using cultured cells. The treatment of DTT increased cell survival after gamma-irradiation, decreased in the frequencies of micronucleus, and reduction in DNA fragmentation and apoptotic cells. Induction of apoptosis after irradiation was revealed by the changes in the relative cell death, increase in the relative amount of apoptotic cells, and the induction of DNA fragmentation. Samples shown excellent radioprotective or radiosensitizing activities will be subjected to purification to develop radioprotectants and radiosensitizers applicable to human beings. The result of mechanism studies will be applied to elucidate the radioprotection mechanism and the develop enhanced radioprotectors. (author). 145 refs., 21 tabs., 29 figs.}
place = {Korea, Republic of}
year = {1999}
month = {Apr}
}