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Biochemical and hematological indicators in model of total body irradiation; Indicadores bioquimicos y hematologicos en un modelo de irradiacion corporal total

Abstract

With the purpose of evaluating the applicability of several biological indicators in accidental overexposures a study was carried out in 20 patients undergoing therapeutical total body irradiation (TBI). The following parameters were evaluated: a) Oxidative stress indicators: erythrocyte superoxide dismutase (SOD) and catalase activity (CAT), lipo peroxyde levels (TBARS) and total plasma antioxidant activity (TAA). b) Haematological indicators: reticulocyte maturity index (RMI) and charges in lymphocyte subpopulations. Non significant changes in SOD and CAT activity were observed. Significant higher TBARS levels were found in patients with unfavorable post-BTM course without any significant correlation with TAA. RMI decreased early and dropped to zero in most of the patients and rose several days prior to reticulocyte, neutrophils and platelets counts. A significant decrease in absolute counts of all lymphocyte subpopulations was observed during TBI, particularly for B lymphocytes. A subpopulation of natural killer (NK) cells (CD16+/ CD 56 +) showed a relative higher radioresistance. Cytotoxic activity was significantly decreased after TBI. These data suggest that TBARS could provide an useful evolutive indicator in accidental over exposure d patients and RMI is an early indicator of bone marrow recovery after radioinduced aplasia. The implications of the different radiosensitivities within the NK subsets remains  More>>
Authors:
Dubner, D; Gisone, P; Perez, M R; [1]  Barboza, M; [2]  Luchetta, P; Longoni, H; Sorrentino, M; Robison, A [3] 
  1. Autoridad Regulatoria Nuclear, Buenos Aires (Argentina)
  2. Hospital de Clinicas Jose de San Martin, Buenos Aires (Argentina)
  3. Hospital Naval Pedro Mallo, Buenos Aires (Argentina)
Publication Date:
Jul 01, 1998
Product Type:
Technical Report
Report Number:
ARN-PI-27/98
Reference Number:
EDB-00:101775
Resource Relation:
Conference: 4. Regional congress on radiological and nuclear safety; Regional Congress IRPA, 4. Congreso regional en seguridad radiologica y nuclear, La Habana (Cuba), 19-23 Oct 1998; Other Information: 6 refs., 2 figs., 1 tab; PBD: 1998
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOCHEMISTRY; BIOLOGICAL INDICATORS; BIOLOGICAL RADIATION EFFECTS; BONE MARROW; HEMATOLOGY; LIPOPROTEINS; NATURAL KILLER CELLS; PATIENTS; PEROXY RADICALS; RADIATION ACCIDENTS; RETICULOCYTES; WHOLE-BODY IRRADIATION
OSTI ID:
20024839
Research Organizations:
Autoridad Regulatoria Nuclear, Buenos Aires (Argentina)
Country of Origin:
Argentina
Language:
Spanish
Other Identifying Numbers:
TRN: AR9900027060047
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
7 pages
Announcement Date:

Citation Formats

Dubner, D, Gisone, P, Perez, M R, Barboza, M, Luchetta, P, Longoni, H, Sorrentino, M, and Robison, A. Biochemical and hematological indicators in model of total body irradiation; Indicadores bioquimicos y hematologicos en un modelo de irradiacion corporal total. Argentina: N. p., 1998. Web.
Dubner, D, Gisone, P, Perez, M R, Barboza, M, Luchetta, P, Longoni, H, Sorrentino, M, & Robison, A. Biochemical and hematological indicators in model of total body irradiation; Indicadores bioquimicos y hematologicos en un modelo de irradiacion corporal total. Argentina.
Dubner, D, Gisone, P, Perez, M R, Barboza, M, Luchetta, P, Longoni, H, Sorrentino, M, and Robison, A. 1998. "Biochemical and hematological indicators in model of total body irradiation; Indicadores bioquimicos y hematologicos en un modelo de irradiacion corporal total." Argentina.
@misc{etde_20024839,
title = {Biochemical and hematological indicators in model of total body irradiation; Indicadores bioquimicos y hematologicos en un modelo de irradiacion corporal total}
author = {Dubner, D, Gisone, P, Perez, M R, Barboza, M, Luchetta, P, Longoni, H, Sorrentino, M, and Robison, A}
abstractNote = {With the purpose of evaluating the applicability of several biological indicators in accidental overexposures a study was carried out in 20 patients undergoing therapeutical total body irradiation (TBI). The following parameters were evaluated: a) Oxidative stress indicators: erythrocyte superoxide dismutase (SOD) and catalase activity (CAT), lipo peroxyde levels (TBARS) and total plasma antioxidant activity (TAA). b) Haematological indicators: reticulocyte maturity index (RMI) and charges in lymphocyte subpopulations. Non significant changes in SOD and CAT activity were observed. Significant higher TBARS levels were found in patients with unfavorable post-BTM course without any significant correlation with TAA. RMI decreased early and dropped to zero in most of the patients and rose several days prior to reticulocyte, neutrophils and platelets counts. A significant decrease in absolute counts of all lymphocyte subpopulations was observed during TBI, particularly for B lymphocytes. A subpopulation of natural killer (NK) cells (CD16+/ CD 56 +) showed a relative higher radioresistance. Cytotoxic activity was significantly decreased after TBI. These data suggest that TBARS could provide an useful evolutive indicator in accidental over exposure d patients and RMI is an early indicator of bone marrow recovery after radioinduced aplasia. The implications of the different radiosensitivities within the NK subsets remains unanswered. (author) [Spanish] Se presentan los resultados de un estudio multiparametrico realizado en pacientes sometidos a irradiacion corporal total (ICT) a fin de evaluar al aplicabilidad de ciertos indicadores bioquimicos y hematologicos en personas accidentalmente sobreexpuestas a radiaciones ionizantes. El trabajo se realizo en pacientes incluidos en un protocolo de irradiacion terapeutica (12 Gy / 4 dias) previo a un transplante de medula osea (TMO). Se tomaron muestras secuenciales de sangre periferica hasta el alta del paciente. Se evaluaron: a) Stress oxidativo: actividad intraeritrocitaria de superoxido dismutasa (SOD) y catalasa (CAT), lipoperoxidos (TBARS) y capacidad antioxidante total del plasma (TRAP). b) Indicadores hematologicos: analisis de reticulocitos por citometria de flujo y determinacion del indice de madurez reticulocitaria (IMR). Abundancia relativa de subpoblaciones linfocitarias y estudio de la actividad funcional citotoxica. Se correlacionaron los resultados obtenidos con la evolucion clinica de los pacientes. No se observaron variaciones significativas en la actividad SOD y CAT. Luego de un incremento inicial, el nivel de TBARS descendio en los pacientes con evolucion favorable. Los valores de TRAP disminuyeron durante el condicionamiento con recuperacion posterior. Los reticulocitos disminuyeron precozmente. La reaparicion de formas inmaduras (corrimiento del IMR) resulto el indicador mas temprano de recuperacion medular. Durante el condicionamiento radiante, todas las subpoblaciones de linfocitos mostraron una caida en los valores absolutos, particularmente los linfocitos B. Un subtipo de linfocitos NK mostraron una relativa radiorresistencia. Se observo una disminucion de la actividad citotoxica. Se discute la aplicabilidad de estos indicadores en el marco de una irradiacion accidental. (autor)}
place = {Argentina}
year = {1998}
month = {Jul}
}