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Prenatal irradiation: radioinduced apoptosis in developing central nervous system; Irradiacion prenatal: apoptosis radioinducida en el sistema nervioso central en desarrollo

Abstract

Severe mental retardation (SMR) is the most significant effect of prenatal irradiation. The high radiosensitivity of developing brain is related with the chronology of morpho genetic phenomena regarding neuroblast proliferation, neuronal differentiation and migration, synaptogenesis and dendritic arborization. Programmed cell death (apoptosis) normally occurs during development in central nervous system (CNS). Apoptosis is a direct result of the expression of specific genes with a final common pathway leading to a characteristic DNA fragmentation pattern. A wide variety of situations and toxic agents have been reported to result in apoptotic death in developing CNS. The aim of this work was the characterization and quantification of apoptosis using an in vitro model of prenatal irradiation. Primary cell cultures from rat brain cortex of 17 days g.a. were irradiated with a gamma source, with doses between 0.2 Gy to 2 Gy. Apoptosis was evaluated 4 hours and 20 hours after irradiation by hematoxylin/eosin, fluorescent microscopy, flow cytometry and DNA electrophoresis. It was also evaluated the neuro protective effect of L-NAME, SOD and glutathion. A dose-dependent increase in apoptotic cell fraction was observed. A protector effect related with the presence of glutathion was observed. (author) [Spanish] Las exposiciones prenatales son motivo frecuente de consulta  More>>
Authors:
Gisone, P; Dubner, D; Michelin, S; Perez, M R; [1]  Barboza, M [2] 
  1. Autoridad Regulatoria Nuclear, Buenos Aires (Argentina)
  2. Hospital de Clinicas Jose de San Martin, Buenos Aires (Argentina)
Publication Date:
Jul 01, 1998
Product Type:
Technical Report
Report Number:
ARN-PI-26/98
Reference Number:
EDB-00:100847
Resource Relation:
Conference: 4. Regional congress on radiological and nuclear safety; Regional Congress IRPA, 4. Congreso regional en seguridad radiologica y nuclear, La Habana (Cuba), 19-23 Oct 1998; Other Information: 15 refs., 3 figs; PBD: 1998
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BRAIN; CELL CULTURES; CELL KILLING; GAMMA SOURCES; IN VITRO; PREGNANCY; PRENATAL IRRADIATION; RADIOSENSITIVITY; RATS
OSTI ID:
20019874
Research Organizations:
Autoridad Regulatoria Nuclear, Buenos Aires (Argentina)
Country of Origin:
Argentina
Language:
Spanish
Other Identifying Numbers:
TRN: AR9900026057643
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
7 pages
Announcement Date:

Citation Formats

Gisone, P, Dubner, D, Michelin, S, Perez, M R, and Barboza, M. Prenatal irradiation: radioinduced apoptosis in developing central nervous system; Irradiacion prenatal: apoptosis radioinducida en el sistema nervioso central en desarrollo. Argentina: N. p., 1998. Web.
Gisone, P, Dubner, D, Michelin, S, Perez, M R, & Barboza, M. Prenatal irradiation: radioinduced apoptosis in developing central nervous system; Irradiacion prenatal: apoptosis radioinducida en el sistema nervioso central en desarrollo. Argentina.
Gisone, P, Dubner, D, Michelin, S, Perez, M R, and Barboza, M. 1998. "Prenatal irradiation: radioinduced apoptosis in developing central nervous system; Irradiacion prenatal: apoptosis radioinducida en el sistema nervioso central en desarrollo." Argentina.
@misc{etde_20019874,
title = {Prenatal irradiation: radioinduced apoptosis in developing central nervous system; Irradiacion prenatal: apoptosis radioinducida en el sistema nervioso central en desarrollo}
author = {Gisone, P, Dubner, D, Michelin, S, Perez, M R, and Barboza, M}
abstractNote = {Severe mental retardation (SMR) is the most significant effect of prenatal irradiation. The high radiosensitivity of developing brain is related with the chronology of morpho genetic phenomena regarding neuroblast proliferation, neuronal differentiation and migration, synaptogenesis and dendritic arborization. Programmed cell death (apoptosis) normally occurs during development in central nervous system (CNS). Apoptosis is a direct result of the expression of specific genes with a final common pathway leading to a characteristic DNA fragmentation pattern. A wide variety of situations and toxic agents have been reported to result in apoptotic death in developing CNS. The aim of this work was the characterization and quantification of apoptosis using an in vitro model of prenatal irradiation. Primary cell cultures from rat brain cortex of 17 days g.a. were irradiated with a gamma source, with doses between 0.2 Gy to 2 Gy. Apoptosis was evaluated 4 hours and 20 hours after irradiation by hematoxylin/eosin, fluorescent microscopy, flow cytometry and DNA electrophoresis. It was also evaluated the neuro protective effect of L-NAME, SOD and glutathion. A dose-dependent increase in apoptotic cell fraction was observed. A protector effect related with the presence of glutathion was observed. (author) [Spanish] Las exposiciones prenatales son motivo frecuente de consulta en el ambito de la radioproteccion. El sistema nervioso es particularmente vulnerable a la accion de la radiacion durante la vida prenatal, con un momento de maxima radiosensibilidad entre las semanas 8 y 15 de edad gestacional (e.g.), durante la cual la frecuencia de retraso mental severo es del orden del 40% por Sievert. La apoptosis, forma activa de muerte celular programada, constituye un proceso fisiologico esencial durante el desarrollo. Las alteraciones de su regulacion podrian jugar un rol en diversas patologias tales como cancer, inmunodeficiencias y enfermedades neurodegenerativas. Se estudio la produccion de apoptosis en un modelo experimental de irradiacion prenatal. Se irradiaron cultivos primarios de cerebro (rata Wistar, dia 15 de e.g.), en un rango de dosis hasta 2 Gy (Cobalto 60). Se evaluo la respuesta a la incubacion con distintos neuroprotectores. Se estudiaron los siguientes parametros: alteraciones de la morfologia celular, fragmentacion de cromatina y fraccion de celulas apoptoticas. Las celulas irradiadas mostraron condensacion nuclear con presencia de cuerpos apoptoticos. Las corridas electroforeticas evidenciaron un patron de fragmentacion caracteristico ('laddering') con aumento dosis-dependiente en la fraccion de celulas apoptoticas analizadas por citometria de flujo. La incubacion con neuroprotectores no indujo cambios significativos en las concentraciones y los tiempos ensayados. Se analizan los resultados obtenidos. Se discute acerca del rol de la apoptosis, su relacion con la injuria oxidativa y los fenomenos de excitotoxicidad como mecanismo de danio radioinducido en el sistema nervioso central en desarrollo. (autor)}
place = {Argentina}
year = {1998}
month = {Jul}
}