You need JavaScript to view this

Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen

Miscellaneous:

Abstract

By means of isolated hepatocytes from rainbow trout (Oncorhyuchus myskiss), the reference substances 4-chloro-aniline, 2,4-dichlorophenol, dinitro-o-cresol and 4-nitrophenol, the pesticides atrazine and disulfotone, the ichthyotherapeutic malachite green, and landfill leachates were studied for their toxic effect. As new endpoints, ultrastructural and enzyme-biochemical parameters were established, which are suitable as a sensitive test system for acute, subacute, and sublethal pollutant effects. In comparison with conventional toxicity tests, ultrastructural and biochemical studies represent the currently most sensitive methods for demonstrating the (eco-) toxicological potential of pollutants. In all experiments, clear time-effect and dose-effect relationships could be made out. Isolated hepatocytes from the liver of rainbow trout reacted more sensitively than the permanent cell lines R1 and RTG-2 investigated in parallel. In cytotoxicity tests with the fibrocytic fish cell lines R1 and RTG-2 from the liver or gonad of rainbow trout, cytotoxicity data for various organic pollutants and organic and inorganic heavy metal compounds were acquired. The results form the basis of a concept for assessing the ecotoxicological potential of environmental pollutants by means of multi-stage cell tests, which is described in the paper. (orig./VHE) [Deutsch] Mit isolierten Hepatocyten aus Regenbogenforellen (oncorhyuchus myskiss) wurden die Referenzsubstanzen 4-Chloranilin, 2,4-Dichlorphenol, Dinitro-o-kresol und 4-Nitrophenol, die Pestizide  More>>
Authors:
Braunbeck, T [1] 
  1. Heidelberg Univ. (Germany). Zoologisches Inst. 1
Publication Date:
Jun 01, 1994
Product Type:
Miscellaneous
Report Number:
CONF-9403248-
Reference Number:
SCA: 560300; 540320; PA: DE-95:0GN548; EDB-96:021630; SN: 96001521000
Resource Relation:
Conference: 2. status colloquium of the project applied ecology, 2. Statuskolloquium des Projektes Angewandte Oekologie, Ettlingen (Germany), 22-23 Mar 1994; Other Information: PBD: Jun 1994; Related Information: Is Part Of 2. status colloquium of the project `Applied ecology` (PAOe). Proceedings; Breitenstein, A. [comp.]; Scholz, W. [comp.]; Waitzmann, M. [comp.]; Zelesny, H. [comp.]; PB: 655 p.; 2. Statuskolloquium des Projektes ``Angewandte Oekologie`` (PAOe). Referate und Beitraege; Veroeffentlichungen Projekt ``Angewandte Oekologie``, v. 8
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 54 ENVIRONMENTAL SCIENCES; FISHES; LIVER CELLS; POLLUTANTS; TOXICITY; BIOLOGICAL INDICATORS; CELL CULTURES; TROUT; ORGANIC COMPOUNDS; METALS
OSTI ID:
164514
Research Organizations:
Landesanstalt fuer Umweltschutz Baden-Wuerttemberg, Karlsruhe (Germany)
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: ISSN 0943-0660; Other: ISBN 3-88251-199-0; TRN: DE95GN548
Availability:
Available from FIZ Karlsruhe
Submitting Site:
DE
Size:
pp. 533-558
Announcement Date:
Jan 17, 2004

Miscellaneous:

Citation Formats

Braunbeck, T. Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen. Germany: N. p., 1994. Web.
Braunbeck, T. Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen. Germany.
Braunbeck, T. 1994. "Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen." Germany.
@misc{etde_164514,
title = {Development of biotest assays using cell cultures from fish for the demonstration of lethal and sublethal damage to organisms due to environmental pollutants in water. Cellular biomarkers in fish cell cultures; Entwicklung von Biotestverfahren mit Zellkulturen aus Fischen zum Nachweis letaler und subletaler Schaeden von Organismen durch Umweltschadstoffe im Wasser. Zellulaere Biomarker in Fischzellkulturen}
author = {Braunbeck, T}
abstractNote = {By means of isolated hepatocytes from rainbow trout (Oncorhyuchus myskiss), the reference substances 4-chloro-aniline, 2,4-dichlorophenol, dinitro-o-cresol and 4-nitrophenol, the pesticides atrazine and disulfotone, the ichthyotherapeutic malachite green, and landfill leachates were studied for their toxic effect. As new endpoints, ultrastructural and enzyme-biochemical parameters were established, which are suitable as a sensitive test system for acute, subacute, and sublethal pollutant effects. In comparison with conventional toxicity tests, ultrastructural and biochemical studies represent the currently most sensitive methods for demonstrating the (eco-) toxicological potential of pollutants. In all experiments, clear time-effect and dose-effect relationships could be made out. Isolated hepatocytes from the liver of rainbow trout reacted more sensitively than the permanent cell lines R1 and RTG-2 investigated in parallel. In cytotoxicity tests with the fibrocytic fish cell lines R1 and RTG-2 from the liver or gonad of rainbow trout, cytotoxicity data for various organic pollutants and organic and inorganic heavy metal compounds were acquired. The results form the basis of a concept for assessing the ecotoxicological potential of environmental pollutants by means of multi-stage cell tests, which is described in the paper. (orig./VHE) [Deutsch] Mit isolierten Hepatocyten aus Regenbogenforellen (oncorhyuchus myskiss) wurden die Referenzsubstanzen 4-Chloranilin, 2,4-Dichlorphenol, Dinitro-o-kresol und 4-Nitrophenol, die Pestizide Atrazin und Disulfoton, das Fischtherapeutikum Malachitgruen sowie Deponiesickerwaesser auf toxische Wirkungen untersucht. Als neuer Endpunkt wurden ultrastrukturelle und enzymbiochemische Parameter ermittelt, die sich als empfindliches Nachweissystem fuer akute, subakute und subletale Schadstoffwirkungen eignen. Im Vergleich mit konventionellen Toxizitaetstests stellen ultrastrukturelle und biochemischen Studien die derzeit empfindlichsten Methoden zum Nachweis des (oeko-)toxikologischen Potential von Schadstoffen dar. In allen Experimenten konnten klare Zeit-Wirkungs- und Dosis-Wirkungs-Beziehungen aufgestellt werden. Isolierte Hepatocyten aus der Leber der Regebogenforelle reagieren empfindlicher als die parallel untersuchten permanenten Zellinien R1 und RTG-2. In Cytotoxizitaetstests mit den bibrocytischen Fischzellinien R1 und RTG-2 aus der Leber bzw. der Gonade der Regenbogenforelle wurden fuer verschiedene organische Schadstoffe und organische und anorganische Schwermetallverbindungen Cytotoxizitaetsdaten erhoben. Diese Erkenntnisse wurden einem Konzept fuer den Einsatz von mehrstugigen Zelltests zur Abschaetzung des oekotoxikologischen Potentials von Umweltschadstoffen zugrunde gelegt, das hier vorgestellt wird. (orig./VHE)}
place = {Germany}
year = {1994}
month = {Jun}
}