Abstract
Flow cytometry allows the rapid measurement of DNA content of single chromosomes or cell nuclei at very high resolution on statistically significant sample. The DNA measurements are usually displayed in form of frequency distribution histograms. Gaussian curves result from the analysis of an homogeneous cell population. Conventionally this dispersion is expressed in terms of relative standard deviation or coefficient of variation (CV). This parameter is constant for an given cellular population at fixed experimental and instrumental conditions. Variation of CV has been widely used to monitor mutagenic effects of physical and chemical treatments; in fact, treatments inducing chromosomal aberrations and disfunctions of mitotic apparatus lead to an unequal distribution of DNA in the daughter cells and, consequently, to an increased variation of the cellular DNA content in the whole cell population. Briefly, evaluation of in situ DNA resistance to denaturation can be evaluated by flow cytometric analysis of different staining pattern of single versus.
Citation Formats
Spano`, M, Leonardi, M, and Cordelli, E.
Use of flow cytometry for possible identification of radio-induced changes in DNA of animal cells.
Italy: N. p.,
1990.
Web.
Spano`, M, Leonardi, M, & Cordelli, E.
Use of flow cytometry for possible identification of radio-induced changes in DNA of animal cells.
Italy.
Spano`, M, Leonardi, M, and Cordelli, E.
1990.
"Use of flow cytometry for possible identification of radio-induced changes in DNA of animal cells."
Italy.
@misc{etde_10150579,
title = {Use of flow cytometry for possible identification of radio-induced changes in DNA of animal cells}
author = {Spano`, M, Leonardi, M, and Cordelli, E}
abstractNote = {Flow cytometry allows the rapid measurement of DNA content of single chromosomes or cell nuclei at very high resolution on statistically significant sample. The DNA measurements are usually displayed in form of frequency distribution histograms. Gaussian curves result from the analysis of an homogeneous cell population. Conventionally this dispersion is expressed in terms of relative standard deviation or coefficient of variation (CV). This parameter is constant for an given cellular population at fixed experimental and instrumental conditions. Variation of CV has been widely used to monitor mutagenic effects of physical and chemical treatments; in fact, treatments inducing chromosomal aberrations and disfunctions of mitotic apparatus lead to an unequal distribution of DNA in the daughter cells and, consequently, to an increased variation of the cellular DNA content in the whole cell population. Briefly, evaluation of in situ DNA resistance to denaturation can be evaluated by flow cytometric analysis of different staining pattern of single versus.}
place = {Italy}
year = {1990}
month = {Dec}
}
title = {Use of flow cytometry for possible identification of radio-induced changes in DNA of animal cells}
author = {Spano`, M, Leonardi, M, and Cordelli, E}
abstractNote = {Flow cytometry allows the rapid measurement of DNA content of single chromosomes or cell nuclei at very high resolution on statistically significant sample. The DNA measurements are usually displayed in form of frequency distribution histograms. Gaussian curves result from the analysis of an homogeneous cell population. Conventionally this dispersion is expressed in terms of relative standard deviation or coefficient of variation (CV). This parameter is constant for an given cellular population at fixed experimental and instrumental conditions. Variation of CV has been widely used to monitor mutagenic effects of physical and chemical treatments; in fact, treatments inducing chromosomal aberrations and disfunctions of mitotic apparatus lead to an unequal distribution of DNA in the daughter cells and, consequently, to an increased variation of the cellular DNA content in the whole cell population. Briefly, evaluation of in situ DNA resistance to denaturation can be evaluated by flow cytometric analysis of different staining pattern of single versus.}
place = {Italy}
year = {1990}
month = {Dec}
}